Adenovirus Universal
Khoom npe
HWTS-RT017A Adenovirus Universal Nucleic Acid kuaj tau khoom (fluorescence pcr)
Kev kawm
Tib neeg Adenovirus (Hadv) belongs rau tus genus niam txiv adenovirus, uas yog ob-stranded DNA tus kab mob tsis muaj hnab ntawv. Adenoviruses uas tau pom kom deb li deb tau muaj 7 pawg neeg (AG) thiab 67 hom, ntawm uas 55 serotypes yog pathogenic rau tib neeg. Among them, could leading to the respiratory tract infections are mainly group B (Types 3, 7, 11, 14, 16, 21, 50, 55), Group C (Types 1, 2, 5, 6, 57) and Group E (Hom 4), thiab yuav ua rau mob plab hnyuv yog pab pawg f (ntau hom 40 thiab 41) [1-8]. Cov hom sib txawv muaj cov tsos mob sib txawv, tab sis feem ntau yog feem ntau ua pa tawm rau cov kab mob. Cov kab mob ua pa los ntawm kev ua pa rau tib neeg lub cev rau 5% ~ 15% ntawm cov kab mob thoob ntiaj teb kev ua pa [9]. Adenovirus muaj kev chim ntau nyob hauv ntau lub cheeb tsam thiab tuaj yeem kis tau txhua xyoo puag ncig, uas yog nquag rau cov kab mob hauv zos, feem ntau hauv cov tsev kawm ntawv thiab cov tub rog pw.
Tshooj
| Tshaib plab | Adenovirus Universalnucleic acid |
| Nas | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | NASOPSYGRE SMAB,Caj pas ci |
| Ct | ≤38 |
| CV | APR5.0% |
| Lub lab | 300copies / ml |
| Yam tshwj xeeb | A) Kuaj cov tuam txhab cov qauv kev ua haujlwm tsis zoo los ntawm cov khoom siv, thiab kev xeem ua kom muaj raws li qhov yuav tsum tau ua. b) Siv cov khoom siv no kom pom thiab tsis muaj tus kab mob hla, xws li tus kab mob ua npaws, thiab lwm yam) lossis cov kab mob (Streptococcus pneumoniae, Klassiella pneumoniae, pseudomonas aeruginosa, Acinetobacterberannii, Staphylococcus aureus, thiab lwm yam). |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler ®480 REO-SIJHAWM PCR system LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEVs (FQD-96A, HangzhouBioer Tshuab) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad CFX96 RIR TSHUAB RIR TSHUAB, Biorad Cfx Opus 96 Real-Lub Sij Hawm PRR cov tshuab |
Ua Haujlwm Ntws
(1) Pom zoo rho tawm reagent:Macro & Micro-xeem piv qhia REGENT (HWTS-3005-8). Cov kev rho tawm yuav tsum tau ua raws li cov lus qhia. Cov qauv rho tawm yog cov neeg mob'Nasopharyngeal rab ntaj lossis caj pas pob zom cov qauv sau rau ntawm qhov chaw. Ntxiv cov qauv rau hauv cov piv txwv tso tawm reagent los ntawm jiangsu macro & Micro-Test Med-tech Co., Ltd. Sib tov thiab tom qab ntawd invert thiab rov ua kom zoo kom tau txais cov DNA ntawm Txhua qhov hnoos qeev.
(2) Pom zoo rho tawm cov lus qhia:Macro & Micro-xeem Tus vacal DNA / RNA KIT(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) Thiab macro & micro-xeem tsis siv neeg nucleic acid extract (HWTS-3006C, HWTS-3006B).Lub lag luam yuav tsum tau ua nyob rau hauv nruj ua raws li cov lus qhia. Cov qauv coj mus coj ua hluav taws xob ntim yog 200μl, thiab tusPom zoo kom muaj kuabis80μl.
(3) Pom zoo rho tawm reagent: nucleic acid extraction lossis purification reagent (YDP315los ntawm Tiangen Biotech (Beijing) Co., Ltd.Cov., tusKev ua haujlwm yuav tsum tau ua nyob rau hauv nruj ua raws li cov lus qhia. Cov qauv coj mus coj ua hluav taws xob ntim yog 200μl, thiab tusPom zoo kom muaj kuabis80μl.
