Adenovirus Universal
Lub npe khoom
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob adenovirus tib neeg (HAdV) yog ib hom kab mob uas yog los ntawm cov tsiaj txhu hu ua Mammalian adenovirus, uas yog ib hom kab mob DNA uas muaj ob txoj hlua khi tsis muaj lub hau npog. Cov kab mob adenovirus uas tau pom muaj txog tam sim no muaj 7 pawg me (AG) thiab 67 hom, uas muaj 55 hom kab mob uas ua rau tib neeg mob. Ntawm lawv, feem ntau yog pawg B (Hom 3, 7, 11, 14, 16, 21, 50, 55), Pawg C (Hom 1, 2, 5, 6, 57) thiab Pawg E (Hom 4), thiab feem ntau yog pawg F (Hom 40 thiab 41) uas ua rau raws plab. Cov kab mob sib txawv muaj cov tsos mob sib txawv, tab sis feem ntau yog cov kab mob ua pa. Cov kab mob ua pa uas ua rau muaj kab mob ua pa ntawm tib neeg lub cev suav txog 5% ~ 15% ntawm cov kab mob ua pa thoob ntiaj teb, thiab 5% -7% ntawm cov kab mob ua pa thaum yau thoob ntiaj teb [9]. Tus kab mob Adenovirus muaj nyob rau ntau thaj chaw thiab tuaj yeem kis tau thoob plaws hauv ib xyoos, tshwj xeeb tshaj yog nyob rau hauv cov chaw muaj neeg coob coob, uas yooj yim kis tus kab mob hauv zos, feem ntau yog nyob rau hauv tsev kawm ntawv thiab cov chaw pw hav zoov.
Channel
| FAM | adenovirus thoob ntiaj tebnucleic acid |
| ROX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | Nasopharyngeal swab,Daim ntaub so caj pas |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Daim/mL |
| Kev Tshwj Xeeb | a) Sim cov ntaub ntawv tsis zoo ntawm lub tuam txhab los ntawm cov khoom siv, thiab cov txiaj ntsig ntawm kev xeem ua tau raws li qhov yuav tsum tau ua. b) Siv cov khoom siv no los nrhiav thiab tsis muaj kev sib kis nrog lwm cov kab mob ua pa (xws li tus kab mob Influenza A, tus kab mob Influenza B, tus kab mob Respiratory syncytial, tus kab mob Parainfluenza, Rhinovirus, Human metapneumovirus, thiab lwm yam) lossis cov kab mob (Streptococcus pneumoniae, Klebsiella pneumoea, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, thiab lwm yam). |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev Cov Txheej Txheem PCR QuantStudio®5 Real-Time SLAN-96P Cov Txheej Txheem PCR Real-Time (Hongshi Medical Technology Co., Ltd.) Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, HangzhouKev siv tshuab Bioer) MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag (Suzhou Molarray Co., Ltd.) BioRad CFX96 Cov Txheej Txheem PCR Tiag-Lub Sijhawm, BioRad CFX Opus 96 Cov Txheej Txheem PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
(1) Cov tshuaj reagent extraction uas pom zoo:Macro & Micro-Test Sample Release Release Release Reagent (HWTS-3005-8). Kev rho tawm yuav tsum ua raws li cov lus qhia. Cov qauv rho tawm yog cov neeg mob'cov qauv ntawm lub qhov ntswg lossis lub caj pas uas tau sau rau ntawm qhov chaw. Muab cov qauv ntxiv rau hauv cov tshuaj reagent tso tawm qauv los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kom sib tov zoo, muab tso rau hauv chav sov li 5 feeb, tshem tawm thiab tom qab ntawd tig rov qab thiab sib tov zoo kom tau txais DNA ntawm txhua tus qauv.
(2) Cov tshuaj reagent extraction uas pom zoo:Kev Ntsuas Macro & Micro Kab mob sib kis Cov Khoom Siv DNA/RNA(HWM-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).qhov kev ua haujlwm yuav tsum tau ua raws li cov lus qhia nruj me ntsis. Cov qauv uas tau rho tawm yog 200μL, thiab covqhov ntim elution pom zoois80μL.
(3) Cov tshuaj reagent uas pom zoo kom rho tawm: Nucleic Acid Extraction lossis Purification Reagent (YDP)315) los ntawm Tiangen Biotech (Beijing) Co., Ltd., tuskev ua haujlwm yuav tsum ua raws li cov lus qhia nruj me ntsis. Cov qauv uas tau rho tawm yog 200μL, thiab covqhov ntim elution pom zoois80μL.
