Adenovirus Universal
Khoom npe
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Tib neeg adenovirus (HAdV) belongs rau genus Mammalian adenovirus, uas yog ob-kab DNA kab mob tsis muaj lub hnab ntawv. Adenoviruses uas tau pom txog tam sim no suav nrog 7 pawg (AG) thiab 67 hom, ntawm 55 serotypes yog kab mob rau tib neeg. Ntawm lawv, tuaj yeem ua rau muaj kab mob ua pa yog feem ntau yog pab pawg B (Tom 3, 7, 11, 14, 16, 21, 50, 55), Pawg C (Types 1, 2, 5, 6, 57) thiab Pawg E (Type 4), thiab tuaj yeem ua rau mob plab hnyuv thiab kab mob F0. 41) [1-8] ib. Ntau hom muaj cov tsos mob sib txawv, tab sis feem ntau yog kab mob ua pa. Cov kab mob ua pa los ntawm cov kab mob ua pa ntawm tib neeg lub cev suav txog 5% ~ 15% ntawm cov kab mob ua pa thoob ntiaj teb, thiab 5% -7% ntawm cov kab mob ua pa thoob ntiaj teb[9]. Adenovirus muaj nyob rau ntau thaj chaw thiab tuaj yeem kis tau txhua xyoo puag ncig, tshwj xeeb tshaj yog nyob rau hauv cov chaw muaj neeg coob coob, uas ua rau muaj kev sib kis hauv zos, feem ntau hauv tsev kawm ntawv thiab cov tub rog camp.
Channel
| FAM | adenovirus universalnucleic acid |
| ROX | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 12 hli |
| Hom Qauv | Nasopharyngeal swb,caj pas swab |
| Ct | ≤38 |
| CV | ≤ 5.0% |
| LoD | 300 daim / ml |
| Qhov tshwj xeeb | a) Ntsuam xyuas lub tuam txhab tus qauv tsis zoo los ntawm cov khoom siv, thiab cov txiaj ntsig kev xeem ua tau raws li qhov yuav tsum tau ua. b) Siv cov khoom siv no txhawm rau kuaj thiab tsis muaj qhov cuam tshuam nrog lwm cov kab mob ua pa (xws li Influenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) los yog kab mob (Streptococcus pneumoniae, K. aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, thiab lwm yam). |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, HangzhouBioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR Systems, BioRad CFX Opus 96 Real-Time PCR Systems |
Ua hauj lwm Flow
(1) Pom zoo extraction reagent:Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kev rho tawm yuav tsum tau ua raws li cov lus qhia. Cov qauv muab rho tawm yog cov neeg mob'nasopharyngeal swab los yog caj pas swab kuaj sau rau ntawm qhov chaw. Ntxiv cov qauv rau hauv cov qauv tso tawm reagent los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex kom sib tov zoo, tso rau hauv chav sov li 5 feeb, tshem tawm thiab rov qab thiab sib tov zoo kom tau txais DNA ntawm txhua tus qauv.
(2) Pom zoo extraction reagent:Macro & Micro-Test Viral DNA/RNA Kit(HWM-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).kev khiav hauj lwm yuav tsum tau ua raws li nruj raws li cov lus qhia. Qhov ntim tau muab rho tawm yog 200μL, thiab covpom zoo elution ntimis80l ua.
(3) Pom zoo extraction reagent: Nucleic Acid Extraction lossis Purification Reagent (YDP315) los ntawm Tiangen Biotech (Beijing) Co., Ltd., covkev khiav hauj lwm yuav tsum tau ua raws li nruj raws li cov lus qhia. Qhov ntim tau muab rho tawm yog 200μL, thiab covpom zoo elution ntimis80l ua.
