ALDH Genetic Polymorphism
Lub npe khoom
HWTS-GE015Cov Khoom Siv Tshawb Nrhiav Kev Hloov Pauv ntawm ALDH Genetic (ARMS -PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
ALDH2 gene (acetaldehyde dehydrogenase 2), nyob rau ntawm tib neeg lub chromosome 12. ALDH2 muaj esterase, dehydrogenase thiab reductase ua haujlwm tib lub sijhawm. Kev tshawb fawb tau qhia tias ALDH2 yog ib qho enzyme metabolic ntawm nitroglycerin, uas hloov nitroglycerin mus rau hauv nitric oxide, yog li ua rau cov hlab ntsha so thiab txhim kho cov ntshav ntws tsis zoo. Txawm li cas los xij, muaj polymorphisms hauv ALDH2 gene, uas feem ntau yog concentrated nyob rau sab hnub tuaj Asia. Hom tsiaj qus ALDH2 * 1 / * 1 GG muaj peev xwm metabolic muaj zog, thaum hom heterozygous tsuas muaj 6% ntawm cov enzyme qus, thiab hom homozygous mutant muaj yuav luag xoom enzyme ua haujlwm, nrog rau metabolism tsis muaj zog heev thiab tsis tuaj yeem ua tiav qhov xav tau, yog li ua rau muaj kev puas tsuaj rau tib neeg lub cev.
Channel
| FAM | ALDH2 |
| ROX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | EDTA tshuaj tiv thaiv cov ntshav txhaws |
| CV | <5.0% |
| LoD | 103Cov ntawv theej/mL |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Lub Tshuab PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm SLAN-96P Cov Txheej Txheem PCR Real-Time Lub Teeb Cicler®480 Lub Sijhawm PCR tiag tiag LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagents uas pom zoo kom rho tawm: siv Blood Genome DNA Extraction Kit (DP318) los ntawm Tiangen Biotech (Beijing) Co.,Ltd. lossis Blood Genome Extraction Kit (A1120) los ntawm Promega los rho tawm EDTA anticoagulated blood Genomic DNA.
Cov tshuaj reagents uas pom zoo kom rho tawm: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (uas siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kev rho tawm yuav tsum tau ua raws li cov lus qhia nruj me ntsis. Qhov ntim elution pom zoo yog100μL.
