Carbapenem Kuj yog noob (KPC / NDM / Oxa 48 / Oxa 23 / Vim li cas / Ox)
Khoom npe
HWTS-OT045 carbapenem Kuj noob (KPC / NDM / NDM / Oxa 48 / Vim li cas 38 / Oxa 23 / Vim li cas 3. Imp) Nrhiav cov khoom siv (Fluorescence pcr)
Kev kawm
Cov tshuaj tua kab mob carbapenem yog atypical β-lactam tshuaj tua kab mob nrog cov tshuaj tua kab mob dav dav thiab muaj zog tshaj tawm tsam. Vim tias nws txoj kev ruaj khov rau β-lactamase thiab tsawg toxicity, nws tau dhau los ua cov tshuaj tiv thaiv kab mob tseem ceeb tshaj plaws ntawm cov kab mob kab mob. Carbapeenems tau ruaj khov rau plasmid-mediated ncua-spectrum β-lloms (Eslosomes, thiab plasmid-deliated cephaporinases (ampc enzymes (ampc enzymes (ampc enzymes (ampc enzymes (ampc enzymes).
Tshooj
| PCR-sib xyaw 1 | PCR-sib tov 2 | |
| Tshaib plab | Tus uas haum | Vim |
| Vic / hex | Sab hauv tswj | Sab hauv tswj |
| Cy5 | Ndm | KPC |
| Nas | Oxa48
| Oxa23 |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | SPUTUM, NTSIAB KHWV, SECTAL SWAB |
| Ct | I≤36 |
| CV | APR5.0% |
| Lub lab | 103CFU / ML |
| Yam tshwj xeeb | A) cov khoom kuaj pom cov qauv tuam txhab tsis zoo, thiab cov txiaj ntsig tau ua tiav ntawm cov ntawv sau ua ke. b) Cov txiaj ntsig ntawm tus ntoo khaub lig-reactivity xeem pom tias cov khoom siv no tsis muaj lwm yam kev tawm tsam, kab mob pob zeb, KlasTocella Oxytoca, Haemophilus Cov neeg ua npaws, Acinetobacter Haemolericus, tus neeg mob siab tawv, tshuaj tiv thaiv kab mob fluorirus, Meca, SME, She, Shr, Shv, Tawv, thiab lwm yam. c) Kev cuam tshuam los tiv thaiv: Mucin, Minocycline, cov lus qhia, Meropofling Seem, thiab cov txiaj ntsig qhia tau hais tias cov khoom cuam tshuam tau sib cuam tshuam tau tsis muaj kev cuam tshuam Txhawm rau kom pom kev ntawm carbapenem tsis kam GENES KPC, NDM, OXA48, OXA23, vim, thiab IM. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler ®480 REO-SIJHAWM PCR system Linegene 9600 Ntxiv rau cov sijhawm tshawb xyuas lub sijhawm tiag tiag (FQD-96A,HangzhouBioer Tshuab) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Qhov Xaiv 1.
Pom zoo rho tawm reagent: macro & micro-kev ntsuas dav dav DNA / RNA (HWTS-3019-50, HWTS-3019-32, hwts-3019-48, hwts-3019-96) (Uas tuaj yeem siv nrog Macro & Micro-Test Acid Extraction (HWTS-3006B, LWTS-3006B)) los ntawm Jiangsu Mor-Tech Co., Ltd .. ntxiv 200μl Tus tso cai ua ntej. Cov kauj ruam tom ntej yuav tsum ua raws cov lus qhia rau kev rho tawm, thiab cov lus pom zoo kom pom zoo kom pom zoo yog100μl.
Qhov Xaiv 2.
Pom zoo rho tawm reagent: nucleic acid extraction lossis purification reagent (ydp32) los ntawm kev qhia rau kev siv (YAULPENT) Co., ltd.hming) Co., Ltd. , thiab co kom txog rau thaum lub thallus tau tshem tawm tag nrho). Siv RNEase / DNase haus dej dawb rau kev txaus siab, thiab cov tsiaj vuag volution yog 100μL.
Qhov Xaiv 3.
Pom zoo rho tawm reagent: macro & micro-ntsuas piv txwv tso tawm reagent. Cov hnoos qeev tau yuav tsum tau ntxuav los ntawm kev ntxiv 1mL ntawm cov saline ib txwm rau cov lus nug sab saud-min rau 5 feeb Rau tus ntshiab colony thiab lub qhov muag sib xyaw, ntxiv 50μl ntawm cov piv txwv tso tawm cov khoom siv uas tau hais ncaj qha, thiab cov kauj ruam tom ntej yuav tsum tau muab rho tawm raws li kev qhia rau kev siv.
