Carbapenem Resistance Gene (KPC / NDM / OXA 48 / OXA 23 / VIM / IMP)
Khoom npe
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Detection Kit (Fluorescence PCR)
Kab mob sib kis
Carbapenem tshuaj tua kab mob yog atypical β-lactam cov tshuaj tua kab mob nrog cov kab mob dav tshaj plaws thiab cov tshuaj tua kab mob muaj zog tshaj plaws. Vim tias nws ruaj khov rau β-lactamases thiab tsis muaj tshuaj lom, nws tau dhau los ua ib qho tseem ceeb tshaj plaws tshuaj tua kab mob rau kev kho mob ntawm cov kab mob loj. Carbapenems ruaj khov heev rau plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, thiab plasmid-mediated cephalosporinases (AmpC enzymes).
Channel
| PCR-Mix 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Internal Control | Internal Control |
| CY 5 | NDM | KPC |
| ROX | OXA 48
| OXA 23 |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 12 hli |
| Hom Qauv | Sputum, pure colonies, rectal swab |
| Ct | ≤36 |
| CV | ≤ 5.0% |
| LoD | 103CFU / ml |
| Qhov tshwj xeeb | a) Cov khoom siv kuaj xyuas lub tuam txhab tus qauv tsis zoo cov ntaub ntawv pov thawj, thiab cov txiaj ntsig tau raws li qhov yuav tsum tau muaj ntawm cov ntaub ntawv sib raug. b) Cov txiaj ntsig ntawm kev ntsuas kev sib tw ua rau pom tau tias cov khoom siv no tsis muaj qhov cuam tshuam nrog lwm cov kab mob ua pa, xws li Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcusaureemella, influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, los yog cov qauv uas muaj lwm yam tshuaj SH, SH-resistant, TEM, thiab lwm yam. c) Los tiv thaiv kev cuam tshuam: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin raug xaiv rau kev cuam tshuam kev kuaj, thiab cov txiaj ntsig tau pom tias cov lus hais saum toj no cuam tshuam cov tshuaj tiv thaiv kab mob ntawm cov tshuaj tiv thaiv kab mob, tsis muaj kev cuam tshuam rau PC. NDM, OXA48, OXA23, VIM, and IMP. |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A,HangzhouBioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
Pom zoo extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-301)9-50, HWM-3019-32, HWM-3019-48, HWM-3019-96) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ntxiv 200μL ntawm ib txwm saline rau thallus precipitate. Cov kauj ruam tom ntej yuav tsum ua raws li cov lus qhia rau kev rho tawm, thiab qhov pom zoo elution ntim yog100l miv.
Kev xaiv 2.
Pom zoo extraction reagent: Nucleic Acid Extraction los yog Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Cov extraction yuav tsum tau pib ua nruj me ntsis raws li cov kauj ruam 2 ntawm cov lus qhia rau kev siv (ntxiv 200μL ntawm buffer GA rau thallus precipitate, thiab co kom txog thaum lub thallus precipitate). Siv RNase/DNase dawb dej rau elution, thiab ecommended elution ntim yog 100μL.
Kev xaiv 3.
Pom zoo extraction reagent: Macro & Micro-Test Sample Tso Reagent. Cov hnoos qeev yuav tsum tau ntxuav los ntawm kev ntxiv 1mL ntawm ib txwm saline rau saum toj no-hais kho thallus precipitate, centrifuged ntawm 13000r / min rau 5 feeb, thiab lub supernatant yog muab pov tseg ( khaws 10-20µL ntawm supernatant). Rau cov colony dawb huv thiab qhov quav swab, ntxiv 50μL ntawm cov qauv tso tawm reagent ncaj qha rau saum toj no-hais kho thallus precipitate, thiab cov kauj ruam tom ntej yuav tsum tau muab rho tawm raws li cov lus qhia rau kev siv.
