Cov noob caj noob ces tiv taus Carbapenem (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Lub npe khoom
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Kev Tshawb Fawb Kit (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Cov tshuaj tua kab mob Carbapenem yog cov tshuaj tua kab mob β-lactam uas tsis zoo li lwm cov tshuaj tua kab mob uas muaj cov tshuaj tua kab mob dav dav thiab muaj zog tshaj plaws. Vim nws ruaj khov rau β-lactamase thiab tsis muaj tshuaj lom, nws tau dhau los ua ib qho tshuaj tua kab mob tseem ceeb tshaj plaws rau kev kho cov kab mob loj heev. Carbapenems ruaj khov heev rau plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, thiab plasmid-mediated cephalosporinases (AmpC enzymes).
Channel
| PCR-Sib xyaw 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Kev Tswj Xyuas Sab Hauv | Kev Tswj Xyuas Sab Hauv |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | Cov hnoos qeev, cov kab mob ntshiab, cov ntaub so quav ntawm qhov quav |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Kev Tshwj Xeeb | a) Cov khoom siv ntes tau cov ntaub ntawv tsis zoo ntawm lub tuam txhab, thiab cov txiaj ntsig ua tau raws li qhov yuav tsum tau ua ntawm cov ntaub ntawv sib xws. b) Cov txiaj ntsig ntawm kev sim tshuaj tiv thaiv kab mob qhia tau tias cov khoom siv no tsis muaj kev sib xyaw nrog lwm cov kab mob ua pa, xws li Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, lossis cov qauv uas muaj lwm cov noob tiv thaiv tshuaj CTX, mecA, SME, SHV, TEM, thiab lwm yam. c) Kev Tiv Thaiv Kev Cuam Tshuam: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin raug xaiv rau kev kuaj kev cuam tshuam, thiab cov txiaj ntsig qhia tau tias cov tshuaj cuam tshuam saum toj no tsis muaj kev cuam tshuam rau kev kuaj pom cov noob tiv thaiv carbapenem KPC, NDM, OXA48, OXA23, VIM, thiab IMP. |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Cov Txheej Txheem PCR QuantStudio®5 Real-Time SLAN-96P Cov Txheej Txheem PCR Real-Time (Hongshi Medical Technology Co., Ltd.) Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A,HangzhouKev siv tshuab Bioer) MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag (Suzhou Molarray Co., Ltd.) BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Kev Xaiv 1.
Cov tshuaj reagent rho tawm uas pom zoo: Macro & Micro-Test General DNA/RNA Kit (HWTS-301)9-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (uas siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Ntxiv 200μL ntawm cov dej ntsev rau hauv cov dej thallus. Cov kauj ruam tom ntej yuav tsum ua raws li cov lus qhia rau kev rho tawm, thiab qhov ntim elution pom zoo yog100μL.
Kev Xaiv 2.
Cov tshuaj reagent uas pom zoo kom rho tawm: Nucleic Acid Extraction or Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Yuav tsum pib rho tawm raws li kauj ruam 2 ntawm cov lus qhia siv (ntxiv 200μL ntawm buffer GA rau hauv thallus precipitate, thiab co kom txog thaum thallus raug tshem tawm tag). Siv dej RNase/DNase dawb rau kev elution, thiab qhov ntim elution uas pom zoo yog 100μL.
Kev Xaiv 3.
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Sample Release Reagent. Yuav tsum ntxuav cov hnoos qeev los ntawm kev ntxiv 1mL ntawm cov dej ntsev rau hauv cov dej thallus uas tau kho lawm, thiab centrifuged ntawm 13000r/min rau 5 feeb, thiab muab cov supernatant pov tseg (khaws 10-20µL ntawm cov supernatant). Yog xav tau cov ntaub so ntswg thiab cov ntaub so quav huv si, ntxiv 50μL ntawm cov tshuaj reagent tso tawm ncaj qha rau hauv cov dej thallus uas tau kho lawm, thiab cov kauj ruam tom ntej yuav tsum tau rho tawm raws li cov lus qhia rau kev siv.
