Dengue Virus, Zika Virus thiab Chikungunya Virus Multiplex
Khoom npe
HWTS-FE040 Dengue Virus, Zika Virus thiab Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Dengue fever (DF), uas yog tshwm sim los ntawm tus kab mob dengue (DENV) kab mob, yog ib qho ntawm cov kab mob sib kis ntau tshaj plaws ntawm arbovirus.Nws kis nruab nrab suav nrog Aedes aegypti thiab Aedes albopictus.DF feem ntau muaj nyob rau hauv thaj chaw sov thiab subtropical.DENV belongs rau flavivirus nyob rau hauv flaviviridae, thiab muaj peev xwm muab faib ua 4 serotypes raws li nto antigen.Cov tsos mob ntawm tus kab mob DENV feem ntau muaj xws li mob taub hau, kub taub hau, tsis muaj zog, mob qog nqaij hlav, leukopenia thiab lwm yam, thiab los ntshav, poob siab, mob siab lossis tuag rau qhov mob hnyav.Nyob rau hauv xyoo tas los no, kev hloov pauv huab cua, kev loj hlob hauv nroog, kev loj hlob sai ntawm kev ncig xyuas thiab lwm yam tau muab cov xwm txheej ceev thiab yooj yim dua rau kev sib kis thiab kev sib kis ntawm DF, ua rau muaj kev nthuav dav ntawm thaj tsam ntawm DF.
Channel
| FAM | MP nucleic acid |
| ROX | Internal Control |
Technical Parameters
| Cia | -18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | Fresh serum |
| Ct | ≤38 |
| CV | ≤ 5.0% |
| LoD | 500 daim / ml |
| Qhov tshwj xeeb | Cov txiaj ntsig kev cuam tshuam tau pom tias thaum cov concentration ntawm bilirubin hauv cov ntshav tsis ntau tshaj 168.2μmol / ml, hemoglobin concentration tsim los ntawm hemolysis tsis ntau tshaj 130g / L, cov ntshav lipid concentration tsis ntau tshaj 65 mmol / ml, tag nrho IgG. concentration hauv cov ntshav tsis ntau tshaj 5mg/mL, tsis muaj kev cuam tshuam rau tus kab mob dengue, Zika virus lossis chikungunya tus kab mob kuaj.Tus kab mob siab A, kab mob siab B, kab mob siab C, kab mob Herpes, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile tus kab mob thiab tib neeg genomic serum kuaj tau raug xaiv rau kev kuaj cross-reactivity, thiab cov txiaj ntsig tau pom tias tsis muaj hla cov tshuaj tiv thaiv ntawm cov khoom siv no thiab cov kab mob hais saum toj no. |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR System Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
TIANamp Virus DNA / RNA Cov Khoom Siv (YDP315-R), thiab kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv.Cov qauv muab rho tawm yog 140μL, thiab qhov pom zoo elution ntim yog 60μL.
Kev xaiv 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., thiab kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv.Cov qauv muab rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 80μL.
