Dengue Virus, Tus kab mob Zika thiab Chikungunya Tus Kab Ntxwv Ntau
Khoom npe
HWTS-FE040 DEZA tus kab mob, tus kab mob Zikunge thiab Chikungunya tus kab mob multipx nucleic acid nrhiav tau khoom siv (fluorescence pcr)
Kev kawm
Dengue kub taub hau (DF), uas yog tsim los ntawm Dengue Virus (DENV), yog ib qho ntawm cov kabmob sib kis feem ntau arbovirus sib kis cov kab mob. Nws cov kis tau nruab nrab nrog ededes aegypti thiab edes albopictus. DF feem ntau muaj kev sib kis hauv thaj chaw kub thiab muaj cov huab cua. Denv belongs rau Flavivirus hauv Flaviviridae, thiab tuaj yeem faib ua 4 serotypes raws li etigen. Cov kev kho mob tshwm sim ntawm Denv tus kab mob yog suav nrog mob taub hau, ua npaws, ua kom ntshav, poob siab, mob hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic, hepatic raug mob lossis tuag nyob rau hauv cov neeg mob hnyav. Nyob rau hauv xyoo tas los, kev nyab xeeb hloov pauv, kev nyab xeeb, kev kawm sai sai rau kev xa khoom thiab kis tau kom muaj kev nthuav dav ntawm thaj chaw ntawm DF.
Tshooj
| Tshaib plab | Denv nucleic acid |
| Nas | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | -18 ℃ |
| Txee-Lub Neej | 9 lub hlis |
| Zauv Hom | Fresh Serum |
| Ct | ≤38 |
| CV | <5% |
| Lub lab | 500 daim / ml |
| Yam tshwj xeeb | Kev Ntsuas Cov Ntsiab Lus Qhia tias thaum lub concentration ntawm bilirubin hauv ntshav ntswg tsis ntau tshaj li 65mmol / ml, tag nrho IGG kev pom zoo hauv cov ntshav dej tsis ntau tshaj 5mg / ml, tsis muaj qhov cuam tshuam ntawm tus kab mob Dengue, tus kab mob khaub thuas lossis Chikungunya tus kab mob virus nrhiav. Kab mob siab hom mob tus kab mob Hepatitis B, tus kab mob Hantaver, sab hnub tuaj yeem xaiv rau Cross-Reactivity Xeem Ntawv, thiab cov txiaj ntsig qhia tau tias tsis muaj Hla kev sib txawv ntawm cov khoom siv no thiab cov kab ke hais los saum toj no. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRRICE Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 REF-LUB SIJ HAWM PRRY Lightcycler®480 REO-SIJ HAWM PRRICE LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEV Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Qhov Xaiv 1.
Tianamp Tus Kab Dna / RNA KIT (YDP315-R), thiab rho tawm yuav tsum tau ua nyob rau hauv nruj ua raws li kev qhia rau kev siv. Cov qauv rho tawm ntim yog 140μl, thiab cov lus pom zoo kom pom zoo yog 60μL.
Qhov Xaiv 2.
Macro & micro-xeem dav DNA / RNA cov khoom (HWTS-3017-96) (uas tuaj yeem siv nrog macro & micro-xeem acid extract quav (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-xeem me Co., Ltd., thiab extraction yuav tsum tau ua raws li kev qhia rau kev siv. Cov qauv rho tawm ntim yog 200μL, thiab cov lus pom zoo kom pom zoo yog 80μL.
