Enterovirus Universal, EV71 thiab CoxA16
Khoom npe
HWTS-EV026B-Enterovirus Universal, EV71 thiab CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Txias-dried Enterovirus Universal, EV71 thiab CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE/MDA (HWTS-EV026)
Kab mob sib kis
Tus kab mob Hand-foot-mouth (HFMD) yog ib hom kab mob kis tau zoo heev hauv cov menyuam yaus. Nws feem ntau tshwm sim hauv cov menyuam yaus hnub nyoog qis dua 5 xyoos, thiab tuaj yeem ua rau mob herpes ntawm tes, taw, qhov ncauj thiab lwm qhov chaw, thiab cov menyuam yaus me tuaj yeem ua rau muaj teeb meem xws li myocarditis, pulmonary edema, aseptic meningoencephalitis, thiab lwm yam. Cov menyuam yaus uas muaj mob hnyav hnyav sai sai thiab pheej hmoo tuag yog tsis kho sai.
Tam sim no, 108 serotypes ntawm enteroviruses tau pom, uas tau muab faib ua plaub pawg: A, B, C thiab D. Enteroviruses uas ua rau HFMD muaj ntau yam, tab sis enterovirus 71 (EV71) thiab coxsackievirus A16 (CoxA16) yog qhov tshwm sim tshaj plaws thiab ntxiv rau HFMD, tuaj yeem ua rau mob hnyav hauv nruab nrab paj hlwb, xws li kab mob plawv thiab kab mob. flaccid tuag tes tuag taw.
Channel
| FAM | Enterovirus |
| VIC (HEX) | CoxA 16 |
| ROX | EV 71 |
| CY 5 | Internal tswj |
Technical Parameters
| Cia | Ua kua: ≤-18 ℃ Hauv tsausLyophilization: ≤30 ℃ |
| Txee lub neej | Kua: 9 lub hlisLyophilization: 12 lub hlis |
| Hom Qauv | Cov kab mob caj pas, cov kua dej Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 daim / ml |
| Cov cuab yeej siv tau | Nws tuaj yeem phim cov khoom siv hluav taws xob fluorescent PCR hauv khw.ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems SLAN-96P Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler® 480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Total PCR Solution
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Cov qauv muab rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 80μL.
Pom zoo extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kev rho tawm yuav tsum tau ua raws li phau ntawv qhia. Cov qauv muab rho tawm yog cov tshuaj oropharyngeal swabs lossis cov kua dej herpes ntawm cov neeg mob uas tau sau rau ntawm qhov chaw. Ntxiv cov swabs sau ncaj qha mus rau Macro & Micro-Test Sample Release Reagent, vortex thiab sib tov zoo, tso rau ntawm chav tsev kub li 5 feeb, tshem tawm thiab rov qab thiab sib tov zoo kom tau txais RNA ntawm txhua tus qauv.
Pom zoo extraction reagent: QIAamp Viral RNA Mini Kit (52904) los ntawm QIAGEN los yog Nucleic Acid Extraction lossis Purification Reagent (YDP315-R). Kev rho tawm yuav tsum tau ua raws li nruj raws li phau ntawv qhia.
