Enterovirus Universal, EV71 thiab CoxA16
Lub Npe Khoom
HWTS-EV026B-Enterovirus Universal, EV71 thiab CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Khoom Siv Kuaj Enterovirus Universal, EV71 thiab CoxA16 Nucleic Acid uas tau muab ziab kom qhuav (Fluorescence PCR)
Daim Ntawv Pov Thawj
CE/MDA (HWTS-EV026)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob tes-taw-qhov ncauj (HFMD) yog ib hom kab mob sib kis sai heev rau cov menyuam yaus. Feem ntau nws tshwm sim rau cov menyuam yaus hnub nyoog qis dua 5 xyoos, thiab tuaj yeem ua rau muaj kab mob herpes rau ntawm tes, taw, qhov ncauj thiab lwm qhov chaw, thiab muaj ob peb tug menyuam yaus tuaj yeem ua rau muaj teeb meem xws li mob myocarditis, mob ntsws o, mob meningoencephalitis, thiab lwm yam. Cov menyuam yaus uas muaj mob hnyav yuav mob hnyav sai thiab yuav tuag yog tias tsis kho sai.
Tam sim no, muaj 108 hom kab mob enteroviruses, uas muab faib ua plaub pawg: A, B, C thiab D. Enteroviruses uas ua rau mob HFMD muaj ntau yam, tab sis enterovirus 71 (EV71) thiab coxsackievirus A16 (CoxA16) yog cov kab mob feem ntau thiab ntxiv rau HFMD, tuaj yeem ua rau muaj teeb meem loj ntawm lub paj hlwb xws li mob meningitis, mob encephalitis, thiab mob tuag tes tuag taw.
Channel
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Kev tswj hwm sab hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | Kua: ≤-18 ℃ Hauv qhov tsaus ntujKev ua kom qhuav: ≤30 ℃ |
| Lub sijhawm khaws cia | Kua: 9 lub hlisKev Lyophilization: 12 lub hlis |
| Hom Qauv | Cov qauv ntawm lub qhov ncauj, cov kua dej ntawm tus kab mob Herpes |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Daim/mL |
| Cov Cuab Yeej Siv Tau | Nws tuaj yeem phim cov cuab yeej siv fluorescent PCR tseem ceeb hauv khw.ABI 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm ABI 7500 Cov Txheej Txheem PCR Ceev Ceev SLAN-96P Cov Txheej Txheem PCR Real-Time Cov Txheej Txheem PCR QuantStudio®5 Real-Time LightCycler®480 Cov Txheej Txheem PCR Tiag-Lub Sijhawm LineGene 9600 Plus Cov Tshuab Tshawb Nrhiav PCR Lub Sijhawm Tiag Tiag MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev daws teeb meem PCR tag nrho
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (uas siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kev rho tawm yuav tsum tau ua raws li phau ntawv qhia. Qhov ntim ntawm cov qauv uas tau rho tawm yog 200μL, thiab qhov ntim ntawm cov kua dej uas pom zoo kom rho tawm yog 80μL.
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kev rho tawm yuav tsum tau ua raws li phau ntawv qhia. Cov qauv rho tawm yog cov swabs oropharyngeal lossis cov kua dej herpes ntawm cov neeg mob uas tau sau rau ntawm qhov chaw. Ntxiv cov swabs uas tau sau ncaj qha rau Macro & Micro-Test Sample Release Reagent, vortex thiab sib tov zoo, muab tso rau hauv chav sov li 5 feeb, tshem tawm thiab tom qab ntawd tig thiab sib tov zoo kom tau txais RNA ntawm txhua tus qauv.
Cov tshuaj reagent uas pom zoo kom rho tawm: QIAamp Viral RNA Mini Kit (52904) los ntawm QIAGEN lossis Nucleic Acid Extraction lossis Purification Reagent (YDP315-R). Kev rho tawm yuav tsum tau ua raws li phau ntawv qhia.
