Kab mob Forest Encephalitis
Lub npe khoom
HWTS-FE006 Cov Khoom Siv Tshawb Nrhiav Kab Mob Ntsws Hlav Hav Zoov (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob encephalitis hauv hav zoov (FE), tseem hu ua kab mob encephalitis uas kis los ntawm kab mob tick-borne (Tick-borne encephalitis, TBE), yog ib qho kab mob sib kis sai sai ntawm lub paj hlwb hauv nruab nrab uas tshwm sim los ntawm tus kab mob encephalitis hauv hav zoov. Tus kab mob encephalitis hauv hav zoov yog los ntawm Flavivirus ntawm tsev neeg Flaviviridae. Cov kab mob me me yog puag ncig nrog txoj kab uas hla ntawm 40-50 nm. Qhov hnyav molecular yog li 4 × 106Yog, thiab tus kab mob genome yog ib qho RNA zoo-kev nkag siab, ib txoj hlua[1]. Hauv kev kho mob, nws yog tus yam ntxwv los ntawm kub taub hau siab, mob taub hau, tsis nco qab, pib sai ntawm kev khaus meningeal, thiab cov leeg nqaij tuag tes tuag taw ntawm lub caj dab thiab ceg, thiab muaj tus nqi tuag siab. Kev kuaj mob thaum ntxov thiab sai ntawm tus kab mob encephalitis hav zoov yog qhov tseem ceeb rau kev kho mob ntawm hav zoov encephalitis, thiab kev tsim kom muaj txoj kev kuaj mob yooj yim, tshwj xeeb thiab sai yog qhov tseem ceeb heev hauv kev kuaj mob ntawm hav zoov encephalitis.[1,2].
Channel
| FAM | tus kab mob encephalitis hauv hav zoov nucleic acid |
| ROX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | cov tshuaj serum tshiab |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Daim/mL |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm SLAN-96P Cov Txheej Txheem PCR Real-Time Lub Teeb Cicler®480 Lub Sijhawm PCR tiag tiag LineGene 9600 Plus Cov Tshuab Tshawb Nrhiav PCR Lub Sijhawm Tiag Tiag MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagent pom zoo kom rho tawm: Nucleic Acid Extraction or Purification Kit (YDP315-R) los ntawm Tiangen Biotech (Beijing) Co., Ltd., kev rho tawm yuav tsum tau ua raws li cov lus qhia nruj me ntsis. Qhov ntim ntawm cov qauv rho tawm pom zoo yog 140μL thiab qhov ntim ntawm elution pom zoo yog 60μL.
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006, HWTS-3006C, HWTS-3006B). kev rho tawm yuav tsum tau ua raws li cov lus qhia nruj me ntsis. Qhov ntim ntawm cov qauv rho tawm uas pom zoo yog 200μL thiab qhov ntim ntawm cov kua dej uas pom zoo yog 80μL.
