Forest Encephalitis Virus
Khoom npe
HWTS-FE006 Forest Encephalitis Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Forest encephalitis (FE), tseem hu ua zuam-borne encephalitis (Tick-borne encephalitis, TBE), yog ib qho kab mob sib kis ntawm lub hauv nruab nrab lub paj hlwb uas tshwm sim los ntawm tus kab mob hav zoov encephalitis. Forest encephalitis virus belongs rau genus Flavivirus ntawm Flaviviridae tsev neeg. Cov kab mob no yog spherical nrog ib txoj kab uas hla ntawm 40-50nm. Qhov hnyav molecular yog li 4 × 106Da, thiab tus kab mob genome yog qhov zoo-sensitive, ib leeg-stranded RNA[1]. Clinically, nws yog tus cwj pwm los ntawm kub taub hau, mob taub hau, coma, pib sai ntawm meningeal irritation, thiab cov leeg nqaij tuag tes tuag taw ntawm caj dab thiab caj dab, thiab muaj kev tuag ntau. Kev kuaj mob sai thiab sai ntawm tus kab mob hav zoov encephalitis yog tus yuam sij rau kev kho mob ntawm hav zoov encephalitis, thiab tsim kom muaj ib qho yooj yim, tshwj xeeb thiab ceev etiological kev kuaj mob yog qhov tseem ceeb hauv kev kuaj mob ntawm hav zoov encephalitis.[1,2].
Channel
| FAM | hav zoov encephalitis virus nucleic acid |
| ROX | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | tshiab serum |
| Tt | ≤38 |
| CV | ≤ 5.0% |
| LoD | 500 daim / ml |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Pom zoo extraction reagent: Nucleic Acid Extraction los yog Purification Kit (YDP315-R) los ntawm Tiangen Biotech (Beijing) Co., Ltd., cov extraction yuav tsum tau ua raws li cov lus qhia nruj me ntsis. Qhov pom zoo muab cov qauv ntim yog 140μL thiab qhov pom zoo elution ntim yog 60μL.
Pom zoo extraction reagent: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-30, 60, HWTS-300 HWM-3006B). kev rho tawm yuav tsum tau ua raws li cov lus qhia nruj me ntsis. Qhov pom zoo muab cov qauv ntim yog 200μL thiab qhov pom zoo elution ntim yog 80μL.
