Khov-qhuav Zaire thiab Sudan Ebolavirus Nucleic Acid
Khoom npe
HWTS-FE035-Freeze-dried Zaire thiab Sudan Ebolavirus Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Ebolavirus belongs rau Filoviridae, uas yog ib qho tsis muaj kab mob ib leeg-stranded negative-strand RNA virus. Cov kab mob yog cov kab mob ntev nrog qhov nruab nrab virion ntev ntawm 1000nm thiab txoj kab uas hla ntawm 100nm. Ebolavirus genome yog ib qho tsis zoo-strand RNA nrog qhov loj ntawm 18.9kb, encoding 7 cov qauv proteins thiab 1 cov protein uas tsis yog qauv. Ebolavirus tuaj yeem muab faib ua hom xws li Zaire, Sudan, Bundibugyo, Tai Forest thiab Reston. Ntawm lawv, hom Zaire thiab Sudan hom tau tshaj tawm tias ua rau ntau tus neeg tuag los ntawm kev kis kab mob. EHF (Ebola Hemorrhagic Fever) yog ib hom kab mob hemorrhagic kis los ntawm Ebolavirus. Tib neeg feem ntau kis tau los ntawm kev sib cuag nrog cov kua hauv lub cev, tso tawm thiab tso tawm ntawm cov neeg mob lossis cov tsiaj muaj kab mob, thiab cov tsos mob tshwm sim feem ntau yog ua npaws, los ntshav thiab ntau yam kabmob. EHF muaj kev tuag siab ntawm 50% -90%. Tam sim no, cov txheej txheem ntawm kev kuaj mob Ebolavirus feem ntau yog kuaj hauv chav kuaj, suav nrog ob yam: kev kuaj pom etiological thiab kuaj pom serological. Etiological kuaj pom muaj xws li kuaj cov kab mob antigens hauv cov ntshav kuaj los ntawm ELISA, kuaj cov nucleic acids los ntawm kev nthuav dav xws li RT-PCR, thiab lwm yam, thiab siv cov cell Vero, Hela, thiab lwm yam rau kev cais tus kab mob thiab kab lis kev cai. Kev kuaj xyuas serological suav nrog kev kuaj xyuas cov tshuaj tiv thaiv IgM tshwj xeeb los ntawm kev ntes ELISA, thiab kuaj xyuas cov tshuaj tiv thaiv IgG los ntawm ELISA, immunofluorescence, thiab lwm yam.
Technical Parameters
Cia | ≤30 ℃ |
Txee lub neej | 12 hli |
Hom Qauv | serum, plasma ua |
CV | ≤ 5.0% |
LoD | 500 Daim / μL |
Cov cuab yeej siv tau | Muaj feem xyuam rau hom I detection reagent: Siv Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer thev naus laus zis), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Muaj feem xyuam rau hom II nrhiav pom reagent: EudemonTMAIO800 (HWTS-EQ007) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Ua hauj lwm Flow
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kev rho tawm yuav tsum tau ua raws li cov lus qhia, thiab cov qauv rho tawm yog 200μL thiab qhov pom zoo elution ntim yog 80μL.