Human PML-RARA Fusion Gene Mutation
Khoom npe
HWM-TM017ATib neeg PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Kab mob sib kis
Mob promyelocytic leukemia (APL) yog hom tshwj xeeb ntawm mob myeloid leukemia (AML). Kwv yees li 95% ntawm cov neeg mob APL tau nrog kev hloov pauv cytogenetic tshwj xeeb, xws li t(15; 17) (q22; q21), uas ua rau PML noob ntawm chromosome 15 thiab retinoic acid receptor α gene (RARA) ntawm chromosome 17 fused los tsim PML-gene.RARA. Vim muaj qhov sib txawv ntawm PML gene, PML-RARA fusion noob tuaj yeem muab faib ua hom ntev (L hom), hom luv (S hom) thiab hom sib txawv (V hom), suav txog kwv yees li 55%, 40% thiab 5% feem.
Channel
| FAM | PML-RARA fusion gene |
| ROX | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ Hauv qhov tsaus ntuj |
| Txee lub neej | 9 muaj |
| Hom Qauv | pob txha |
| CV | <5.0% |
| LoD | 1000 Daim / ml. |
| Qhov tshwj xeeb | Tsis muaj kev sib cuam tshuam nrog lwm cov noob fusion BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, thiab TEL-AML1 fusion noob |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR System Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer thev naus laus zis) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Pom zoo extraction reagent: RNAprep Ntshiab Ntshav Tag Nrho RNA Extraction Kit (DP433). Kev rho tawm yuav tsum tau ua raws li IFU.
