Kev Hloov Pauv ntawm Tib Neeg PML-RARA Fusion Gene
Lub npe khoom
HWTS-TM017ACov Khoom Siv Tshawb Nrhiav Kev Hloov Pauv ntawm Cov noob caj noob ces PML-RARA rau Tib Neeg (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob leukemia promyelocytic (APL) yog ib hom kab mob leukemia myeloid (AML) tshwj xeeb. Kwv yees li 95% ntawm cov neeg mob APL muaj kev hloov pauv tshwj xeeb hauv cytogenetic, uas yog t(15;17)(q22;q21), uas ua rau cov noob caj noob ces PML ntawm chromosome 15 thiab cov noob caj noob ces retinoic acid receptor α (RARA) ntawm chromosome 17 sib xyaw ua ke los ua cov noob caj noob ces PML-RARA fusion. Vim muaj ntau qhov chaw sib txawv ntawm cov noob caj noob ces PML, cov noob caj noob ces PML-RARA fusion tuaj yeem faib ua hom ntev (L hom), hom luv (S hom) thiab hom sib txawv (V hom), suav txog kwv yees li 55%, 40% thiab 5% feem.
Channel
| FAM | PML-RARA fusion gene |
| ROX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ Hauv qhov tsaus ntuj |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | pob txha |
| CV | <5.0% |
| LoD | 1000 Daim Ntawv Luam / mL. |
| Kev Tshwj Xeeb | Tsis muaj kev sib tshuam nrog lwm cov noob sib xyaw BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, thiab TEL-AML1 fusion genes. |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Lub Tshuab PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm SLAN-96P Cov Txheej Txheem PCR Real-Time (Hongshi Medical Technology Co., Ltd.) Lub Teeb Cicler®480 Lub Sijhawm PCR tiag tiag LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag (Suzhou Molarray Co., Ltd.) BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagent uas pom zoo kom rho tawm: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Kev rho tawm yuav tsum tau ua raws li IFU.
