Tib neeg PML-Rara Fusion Gene Mutation
Khoom npe
HWTS-TM017ATib neeg PML-RAAH FUUS KAB KWV QAIB MENYUAM nrhiav cov khoom siv (fluorescence pccr)
Kev kawm
Raug Predyelocytic cov lus hais (APL) yog hom tshwj xeeb ntawm Myeleloid leukemia (AML). Txog 95% ntawm cov neeg mob Apl uas muaj kev hloov pauv tshwj xeeb, uas yog qhov ua rau PML rece ntawm chromosome 17 fused rau ua lub pml-rara fusion gene. Vim tias cov noob sib txawv ntawm PML Gene, PML-Rara fusion gene tuaj yeem muab faib ua hom ntev (L Hom) thiab variant rau kwv yees li 55%, 40% thiab 5 % feem.
Tshooj
| Tshaib plab | PML-Rara Fusion Gene |
| Nas | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ hauv kev tsaus ntuj |
| Txee-Lub Neej | 9 lub hlis |
| Zauv Hom | hlwb pob txha |
| CV | <0% |
| Lub lab | 1000 daim ntawv luam / ml. |
| Yam tshwj xeeb | Tsis muaj tus ntoo khaub lig-reactivity nrog lwm cov kev fusion noob bcr-abl, e2a-pbx1, mll-af4, AML1-ETO, thiab TEL-AML1 fusion noob |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRRICE Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 REF-LUB SIJ HAWM PRRY Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler®480 REO-SIJ HAWM PRRICE Linegene 9600 Ntxiv rau Kev Ntsuas Phaj Phaj Real-Time (FQD-96A, Hangzhou Biorer Technology) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Pom zoo rho tawm reagent: rnaprep ntshiab ntshav siab tag nrho RNA Extraction cov khoom (DP433). Cov kev rho tawm yuav tsum tau ua raws li IFU.
