Kabmob tus kab mob tagnrho University University / H1 / H3
Khoom npe
HWTS-RT012 Tus kab mob npaws Tus kabmob virusal / h1 / h1 / h3 nucleic acid modlex nrhiav khoom siv (fluorescence pcr)
Kev kawm
Tus kab mob khaub thuas yog ib hom neeg sawv cev ntawm Orthotomyxoviridae. Nws yog ib kab kev uas muaj kev hem thawj tib neeg kev noj qab haus huv. Nws tuaj yeem sib kis tus tswv tsev dav dav. Cov kabmob raws caij nyoog cuam tshuam txog 600 lab tus tib neeg thoob ntiaj teb thiab ua rau 250,000 tus mob khaub thuas yog qhov ua rau tus mob khaub thuas yog qhov tseem ceeb ntawm kev kis mob thiab tuag. Tus kab mob khaub thuas Tus kab mob no yog ib-tso tseg tsis zoo-Stranded RNA. Raws li nws nto Hemagglutinin (HA) thiab neuraminidase (nA), ha tuaj yeem muab faib ua 16 subtepes, thiab muab faib ua 9 subtypes. Ntawm cov kab mob npaws cov kab mob, cov kab mob khaub thuas subtenza uas tuaj yeem ua rau tib neeg muaj: H3N2, H7N2, H7N2, H7N9, H7N9, H10N2. Ntawm lawv, H1 thiab H3 subtypes yog pathogenic heev, thiab yog tshwj xeeb yog tsim nyog tau txais kev mloog.
Tshooj
| Tshaib plab | Mob khaub thuas ib tug virus virus nucleic acid |
| Vic / hex | Kab mob ua npaws H1 hom kab mob kua qaub |
| Nas | Kab mob khaub thuas H3 hom kab mob nucleic acid |
| Cy5 | sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ |
| Txee-Lub Neej | 9 lub hlis |
| Zauv Hom | NASOPSYGRE SMAB |
| Ct | ≤38 |
| CV | APR5.0% |
| Lub lab | 500 daim / μl |
| Yam tshwj xeeb | Tsis muaj tus ntoo khaub lig-reactivity nrog lwm cov pa ua kom zoo li tus kab mob npaws, B1 / B2, Ua pa Cov kab mob EN / B, Coronavirus 229e / nl63 / hku1 / oc43, rhinovirus A / C2/4, CRAMYDIA Tach, Adlamydia Trachatis, etc. thiab tib neeg genomic DNA. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRRICE Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler ®480 REO-SIJHAWM PCR system Linegene 9600 Ntxiv rau Kev Ntsuas Phaj Phaj Real-Time (FQD-96A, Hangzhou Biorer Technology) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Nucleic acid extraction lossis purification reagent (YDP315-r) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Cov kev rho tawm yuav tsum tau nqa tawm nruj me ntsis raws li cov lus qhia rau kev siv. Cov qauv rho tawm ntim yog 140μl, thiab cov lus pom zoo kom pom zoo yog 60μL.
Qhov Xaiv 2.
Macro & micro-xeem kis DNA / RNA Cov Khoom Siv (HWTS-3004-48, HWTS-3004-96) thiab Macro thiab Macro thiab Macro & Micrours-3006C, HWTS-3006B). Cov kev rho tawm yuav tsum tau nqa tawm nruj me ntsis raws li cov lus qhia rau kev siv. Cov qauv rho tawm ntim yog 200μL, thiab cov lus pom zoo kom pom zoo yog 80μL.
