Klebsiella Pneumoniae, Acinetobacter Baumannii thiab Pseudomonas Aeruginosa thiab Cov noob tiv thaiv tshuaj (KPC, NDM, OXA48 thiab IMP) Multiplex
Lub npe khoom
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii thiab Pseudomonas Aeruginosa thiab Cov noob tiv thaiv tshuaj (KPC, NDM, OXA48 thiab IMP) Multiplex Detection Kit (Fluorescence PCR)
Daim Ntawv Pov Thawj
CE
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Klebsiella pneumoniae yog ib hom kab mob uas feem ntau kis tau los ntawm kev sib kis hauv tsev kho mob thiab yog ib hom kab mob tseem ceeb uas ua rau muaj kev kis kab mob hauv tsev kho mob. Thaum lub cev tsis muaj zog tiv thaiv kab mob lawm, cov kab mob yuav nkag mus rau hauv lub ntsws los ntawm txoj hlab pa, ua rau muaj kev kis kab mob rau ntau qhov chaw ntawm lub cev, thiab kev siv tshuaj tua kab mob thaum ntxov yog qhov tseem ceeb rau kev kho mob.[1].
Qhov chaw uas muaj tus kab mob Acinetobacter baumannii ntau tshaj plaws yog lub ntsws, uas yog ib qho tseem ceeb rau kev mob ntsws hauv tsev kho mob (HAP), tshwj xeeb tshaj yog mob ntsws los ntawm lub tshuab ua pa (VAP). Feem ntau nws muaj lwm yam kab mob bacterial thiab fungal, nrog rau cov yam ntxwv ntawm kev mob nkeeg thiab kev tuag ntau.
Pseudomonas aeruginosa yog cov kab mob gram-negative bacilli uas tsis yog fermentative feem ntau hauv kev kho mob, thiab yog ib qho tseem ceeb rau kev kis tus kab mob hauv tsev kho mob, nrog rau cov yam ntxwv ntawm kev yooj yim colonization, yooj yim variation thiab ntau yam tshuaj tsis kam.
Channel
| Lub Npe | PCR-Sib xyaw 1 | PCR-Mix 2 |
| FAM Channel | Aba | IMP |
| VIC/HEX Channel | Kev Tswj Xyuas Sab Hauv | KPC |
| CY5 Channel | PA | NDM |
| ROX Channel | KPN | OXA48 |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | Cov hnoos qeev |
| Ct | ≤36 |
| CV | ≤10.0% |
| LoD | 1000 CFU/mL |
| Kev Tshwj Xeeb | a) Qhov kev sim tshuaj tiv thaiv kab mob qhia tau hais tias cov khoom siv no tsis muaj kev cuam tshuam nrog lwm cov kab mob ua pa, xws li Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus thiab cov qauv hnoos qeev tsis muaj lub hom phiaj, thiab lwm yam. b) Muaj peev xwm tiv thaiv kev cuam tshuam: Xaiv mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, thiab roxithromycin, thiab lwm yam rau qhov kev xeem cuam tshuam, thiab cov txiaj ntsig qhia tau tias cov tshuaj cuam tshuam uas tau hais los saum toj no tsis cuam tshuam rau kev kuaj pom Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa thiab carbapenem tiv thaiv noob KPC, NDM, OXA48 thiab IMP. |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm Lub Teeb Cicler®480 Lub Sijhawm PCR tiag tiag LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Bioer technology) MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag (Suzhou Molarray Co., Ltd.) BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Kev daws teeb meem PCR tag nrho
