Monkeyppox virus thiab ntaus nucleic acid

Cov lus piav qhia luv luv:

Cov khoom siv no yog siv rau hauv venro txaus paub tab ntawm monkypox virus clade i, clade II thiab monkypox virus univers dej, orophyngeal so thiab mob ntshav.

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Khoom Qhia Qhia

Khoom Ntawv

Khoom npe

HWTS-OT202-MiKypox Virus thiab ntaus nucleic acid nrhiav tau khoom siv (fluorescence pcr)

Daim ntawv muaj cai

Kev kawm

Monkypox (MPOX) yog ib qho kev mob zoonotic kis los ntawm monkypox virus (mpxv). Mpxv yog puag ncig-cib lossis oval nyob rau hauv cov duab, thiab yog ob-stranded DNA tus kab mob nrog lub ntev ntawm kwv yees li 197kb^[1]Cov. Tus kab mob no tsuas yog kis tau los ntawm cov tsiaj txhu los ntawm kev raug kis los ntawm cov tsiaj los yog los ntawm kev sib chwv ntshav, ntawm lub cev thiab tawm ntawm cov tsiaj uas muaj tus kab mob. Tus kab mob kis tau los ntawm cov neeg, feem ntau yog los ntawm kev ua pa lossis los ntawm tus neeg mob lub cev lossis cov khoom tsis huv^[2-3]Cov. Cov kev tshawb fawb tau pom tias MPXV cov ntaub ntawv ob qho sib txawv: Clade I (TUPTLious BASIN TRADE) thiab CONCEAMS tau hu ua West African clade). MPOX ntawm Congo Basin Clade tau raug hloov tau ntawm tib neeg thiab tuaj yeem ua rau tuag taus ua rau cov tsos mob me thiab muaj tus nqi qis dua ntawm tib neeg-rau-tib neeg kis^[4].

Cov khoom siv xeem ntawm cov khoom siv no tsis yog qhov ntsuas pom ntawm Mpxv kis tau rau cov neeg mob mpxv rau cov neeg mob hauv cov kab mob uas yog cov kab mob uas kho tau tus mob Npaj kom ntseeg tau kev nyab xeeb thiab kev kho zoo.

Tshooj

Tshaib plab	Mpxv clade II
Nas	Mpxv Universal nucleic acid
Vic / hex	Mpxv clade i
Cy5	sab hauv tswj

Kev tsis muaj

Chaw ntim khoom	≤-18 ℃
Txee-Lub Neej	12 lub hlis
Zauv Hom	Tib neeg pob taws, orophyngeal so thiab ntshav
Ct	≤38 (Nam, Vic / Hex, Rox), ≤35 (IC)
Lub lab	200 daim ntawv luam / ml
Muaj cov cuab yeej siv tau	Hom Kuv nrhiav tau reagent: Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio^®5 REF-LUB SIJ HAWM PRRY Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler^®480 REO-SIJ HAWM PRRICE Linegene 9600 Ntxiv rau cov sijhawm pr Kev Nrhiav Lus Nrhiav (FQD-96A, Hangzhou Bioer Technology) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System Ntaus ii nrhiav tau reagent: Eudemon^TMAio800 (HWTS-EQ007)) los ntawm Jiangsu Macro & Micro-xeem me Co., Ltd.