Monkeypox Virus thiab Typing Nucleic Acid

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Cov khoom siv no yog siv rau hauv vitro qualitative detection of monkeypox virus clade I, clade II thiab monkeypox virus universal nucleic acids in human rash fluid, oropharyngeal swabs and serum samples.

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Product Detail

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HWTS-OT202-Monkeypox Virus thiab Typing Nucleic Acid Detection Kit (Fluorescence PCR)

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Kab mob sib kis

Monkeypox (Mpox) yog tus kab mob zoonotic kis tau los ntawm Monkeypox Virus (MPXV). MPXV yog puag ncig-bricked los yog oval nyob rau hauv cov duab, thiab yog ib tug ob-stranded DNA kab mob nrog ib tug ntev ntawm txog 197Kb^[1]. Tus kab mob kis tau los ntawm cov tsiaj, thiab tib neeg tuaj yeem kis tau los ntawm kev raug tsiaj los ntawm cov kab mob los ntawm kev sib cuag ncaj qha nrog cov ntshav, kua dej hauv lub cev thiab cov pob liab liab ntawm cov tsiaj txhu. Tus kab mob no tseem tuaj yeem kis tau ntawm tib neeg, feem ntau los ntawm cov pa ua pa thaum lub sij hawm ntev, kev sib cuag nrog lub ntsej muag lossis los ntawm kev sib cuag ncaj qha nrog tus neeg mob cov kua hauv lub cev lossis cov khoom tsis huv.^[2-3]. Cov kev tshawb fawb tau pom tias MPXV tsim ob qhov sib txawv: clade I (yav tas los hu ua Central African clade lossis Congo Basin clade) thiab clade II (yav dhau los hu ua West African clade). mpox ntawm Congo Basin clade tau pom meej meej tias kis tau ntawm tib neeg thiab tuaj yeem ua rau tuag taus, thaum mpox ntawm West African clade ua rau cov tsos mob me me thiab muaj qis dua ntawm tib neeg mus rau tib neeg.^[4].

Cov txiaj ntsig ntawm cov khoom siv no tsis yog tsim los ua ib qho kev taw qhia rau kev kuaj mob MPXV kab mob hauv cov neeg mob, uas yuav tsum tau ua ke nrog tus neeg mob cov yam ntxwv kho mob thiab lwm cov ntaub ntawv kuaj xyuas kom raug txiav txim rau tus kab mob thiab tsim cov kev kho mob tsim nyog kom muaj kev nyab xeeb thiab kev kho mob zoo.

Channel

FAM	MPXV clade II
ROX	MPXV universal nucleic acid
VIC/HEX	MPXV clade I
CY 5	tswj sab hauv

Technical Parameters

Cia	≤-18 ℃
Txee lub neej	12 hli
Hom Qauv	tib neeg cov kua dej tawm pob, oropharyngeal swabs thiab serum
Ct	≤38 (FAM, VIC/HEX, ROX), ≤35 (IC)
LoD	200 daim / ml
Cov cuab yeej siv tau	Hom I detection reagent: Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio^®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler^®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer thev naus laus zis) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System Hom II nrhiav reagent: Eudemon^TMAIO800 (HWTS-EQ007)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.