MTHFR Gene Polymorphic Nucleic Acid
Lub npe khoom
HWTS-GE004-MTHFR Cov Khoom Siv Tshawb Nrhiav Gene Polymorphic Nucleic Acid (ARMS-PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Folic acid yog ib yam vitamin uas yaj tau hauv dej uas yog ib qho tseem ceeb hauv lub cev txoj kev zom zaub mov. Nyob rau xyoo tas los no, muaj ntau txoj kev tshawb fawb tau lees paub tias, kev hloov pauv ntawm cov enzyme folate metabolizing gene MTHFR yuav ua rau lub cev tsis muaj folic acid, thiab kev puas tsuaj ntawm folic acid deficiency rau cov neeg laus yuav ua rau megaloblastic anemia, vascular endothelial puas tsuaj, thiab lwm yam. Folic acid deficiency rau cov poj niam cev xeeb tub tsis tuaj yeem ua tau raws li qhov xav tau ntawm lawv tus kheej thiab tus menyuam hauv plab, uas tuaj yeem ua rau neural tube defects, anencephaly, tuag menyuam, thiab nchuav menyuam. Cov folate hauv cov ntshav raug cuam tshuam los ntawm 5,10-methylenetetrahydrofolate reductase (MTHFR) polymorphisms. Cov kev hloov pauv 677C>T thiab 1298A>C hauv MTHFR gene ua rau kev hloov pauv ntawm alanine mus rau valine thiab glutamic acid, ua rau MTHFR kev ua haujlwm tsawg dua thiab ua rau kev siv folic acid tsawg dua.
Channel
| FAM | MTHFR C677T |
| ROX | MTHFR A1298C |
| VIC (HEX) | Kev tswj hwm sab hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | Cov ntshav EDTA uas tau khaws cia tshiab |
| CV | ≤5.0% |
| Ct | ≤38 |
| LoD | 1.0ng/μL |
| Cov Cuab Yeej Siv Tau: | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev SLAN ®-96P Cov Txheej Txheem PCR Lub Sijhawm Tiag QuantStudio™ 5 Cov Txheej Txheem PCR Lub Sijhawm Tiag Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Kev Xaiv 1
Cov tshuaj reagents uas pom zoo kom rho tawm: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
Kev Xaiv 2
Cov tshuaj reagents uas pom zoo kom rho tawm: Cov Khoom Siv Rho Tawm DNA Genomic Ntshav (YDP348, JCXB20210062) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Cov Khoom Siv Rho Tawm Genome Ntshav (A1120) los ntawm Promega.
