Txij li thaum lub Tsib Hlis 2022, cov neeg mob mpox tau raug tshaj tawm hauv ntau lub tebchaws uas tsis muaj kab mob sib kis thoob plaws ntiaj teb uas muaj kev sib kis hauv zej zog.
Thaum Lub Yim Hli 26, Lub Koom Haum Kev Noj Qab Haus Huv Ntiaj Teb (WHO) tau tshaj tawm thoob ntiaj tebKev Npaj Txhij thiab Kev Npaj Teb rau Txoj Kev Npajkom tsis txhob muaj kev sib kis ntawm tib neeg mus rau tib neeg ntawm mpox los ntawm kev sib koom tes thoob ntiaj teb, cheeb tsam, thiab lub tebchaws. Qhov no ua raws li kev tshaj tawm txog kev kub ntxhov ntawm kev noj qab haus huv pej xeem uas muaj kev txhawj xeeb thoob ntiaj teb los ntawm WHO Director-General rau hnub tim 14 Lub Yim Hli.
Yuav tsum nco ntsoov tias qhov kev sib kis ntawm mpox lub sijhawm no txawv ntawm xyoo 2022, uas feem ntau kis ntawm cov txiv neej uas sib deev nrog txiv neej, thiab tus nqi tuag ntawm cov neeg mob tsawg dua 1%.
Tus kab mob tshiab "Clade Ib", uas yog ib hom kab mob ntawm Clade I, muaj feem pua ntawm kev tuag ntau dua. Tus kab mob tshiab no tau pib kis mus rau DRC thaum lub Cuaj Hli tas los, thaum xub thawj yog cov neeg ua haujlwm sib deev, thiab tam sim no tau kis mus rau lwm pab pawg, nrog rau cov menyuam yaus tshwj xeeb tshaj yog cov uas muaj feem yuav kis tau tus kab mob no.
Lub koom haum Africa CDC tau hais hauv daim ntawv tshaj tawm lub hli tas los tias muaj cov kab mob mpox nyob hauv 10 lub tebchaws African xyoo no, suav nrog DRC, uas tau tshaj tawm txog 96.3% ntawm tag nrho cov neeg mob hauv Africa xyoo no thiab 97% ntawm cov neeg tuag. Nws tsim nyog sau cia tias yuav luag 70% ntawm cov neeg mob hauv DRC yog cov menyuam yaus hnub nyoog qis dua 15 xyoo, thiab pawg no suav txog 85% ntawm cov neeg tuag hauv lub tebchaws.
Kab mob Mpox yog ib hom kab mob sib kis los ntawm tus kab mob mpox uas yuav siv sijhawm li 5 txog 21 hnub, feem ntau yog 6 txog 13 hnub. Tus neeg mob yuav muaj cov tsos mob xws li kub taub hau, mob taub hau thiab o ntawm cov qog ntshav, ua raws li pob khaus ntawm lub ntsej muag thiab lwm qhov ntawm lub cev, uas maj mam loj hlob mus ua pob khaus thiab kav ntev li ib lub lim tiam ua ntej yuav tawm pob khaus. Tus kab mob no kis tau los ntawm qhov pib muaj cov tsos mob mus txog thaum cov pob khaus poob tawm ntawm nws tus kheej.
Macro & Micro-Test muab kev kuaj mob sai, cov khoom siv molecular thiab cov kev daws teeb meem sequencing rau kev kuaj mob mpox tus kab mob, pab kuaj mob mpox tus kab mob raws sijhawm, saib xyuas nws keeb kwm, caj ces, kev sib kis thiab kev hloov pauv genomic:
Cov tshuaj tua kab mob MonkeypoxCov Khoom Siv Tshawb Nrhiav (Kev kuaj mob immunochromatography)
Kev kuaj yooj yim (kuaj kua qaub ncaug/qhwv caj pas) thiab cov txiaj ntsig sai hauv 10-15 feeb;
Muaj kev rhiab heev nrog LoD ntawm 20pg / mL npog Clade I & II;
Muaj kev tshwj xeeb siab uas tsis muaj kev cuam tshuam nrog tus kab mob me me, tus kab mob varicella zoster, tus kab mob rubella, tus kab mob herpes simplex, thiab lwm yam.
OPA ntawm 96.4% piv rau NAATs;
Daim ntawv thov dav xws li kev lis kev cai, CDCs, tsev muag tshuaj, chaw kho mob, tsev kho mob lossis hauv tsev.
Cov Khoom Siv Kuaj Mob IgM/IgG Antibody rau Kab Mob Monkeypox(Immunochromatographyhy)
Kev ua haujlwm yooj yim tsis muaj cuab yeej thiab ua tiav sai hauv 10 feeb;
Kev rhiab heev thiab kev tshwj xeeb uas npog Clade I & II;
Txheeb xyuas IgM thiab IgG los txiav txim siab txog theem ntawm kev kis kab mob mpox;
Daim ntawv thov dav xws li kev lis kev cai, CDCs, tsev muag tshuaj, chaw kho mob, tsev kho mob lossis hauv tsev;
Tsim nyog rau kev tshuaj ntsuam loj ntawm cov kab mob mpox uas xav tias muaj.
Muaj kev rhiab heev nrog LoD ntawm 200 Copies / mL nrog IC, sib npaug rau fluorescence PCR;
Kev ua haujlwm yooj yim: Cov qauv lysed ntxiv rau hauv lub raj mis lyophilized reagent rau kev ua kom nrov ncaj qha los ntawm cov modules ywj pheej ntawm Easy Amp System;
Muaj kev tshwj xeeb siab yam tsis muaj kev cuam tshuam nrog tus kab mob me me, tus kab mob vaccinia, tus kab mob cowpox, tus kab mob mousepox, tus kab mob herpes simplex, tus kab mob varicella-zoster, thiab tib neeg genome, thiab lwm yam.
Kev kuaj yooj yim (cov kua dej tawm pob khaus/siv daim ntaub so qhov ncauj) thiab qhov tshwm sim zoo sai tshaj plaws hauv 5 feeb;
Kev ua tau zoo heev hauv kev kho mob uas npog Clade I & II nrog PPA ntawm 100%, NPA ntawm 100%, OPA ntawm 100% thiab Kappa Value ntawm 1.000 piv nrog Fluorescence PCR kit;
Lyophilized version tsuas yog xav tau kev thauj mus los thiab cia khoom hauv chav sov xwb ua rau nkag tau yooj yim rau txhua thaj chaw;
Cov xwm txheej hloov pauv tau yooj yim hauv cov chaw kho mob, chaw kho mob, nrog rau Easy Amp rau kev kuaj pom thaum xav tau;
Cov Khoom Siv Kuaj Mob Monkeypox Virus Nucleic Acid (Fluorescence PCR)
Ob lub noob caj noob ces tsom mus rau qhov muaj kev rhiab heev nrog LoD ntawm 200 daim ntawv theej / mL;
Kev kuaj mob yooj yim ntawm cov kua qaub ncaug, qhov ntswg caj pas thiab cov ntshav;
Muaj kev tshwj xeeb siab yam tsis muaj kev cuam tshuam nrog tus kab mob me me, tus kab mob vaccinia, tus kab mob cowpox, tus kab mob mousepox, tus kab mob herpes simplex, tus kab mob varicella-zoster, thiab tib neeg genome, thiab lwm yam.
Kev ua haujlwm yooj yim: kev lysis qauv sai los ntawm cov qauv tso tawm reagent kom ntxiv rau hauv lub raj tshuaj tiv thaiv;
Kev kuaj pom sai: tshwm sim hauv 40 feeb;
Kev tseeb raug xyuas los ntawm kev tswj hwm sab hauv uas saib xyuas tag nrho cov txheej txheem kuaj pom;
Kev ua tau zoo heev hauv kev kho mob uas npog Clade I & II nrog PPA ntawm 100%, NPA ntawm 99.40%, OPA ntawm 99.64% thiab Kappa Value ntawm 0.9923 piv nrog kev txheeb xyuas sequencing;
Lyophilized version tsuas yog xav tau kev thauj mus los thiab cia khoom hauv chav sov xwb ua rau nkag tau yooj yim rau txhua thaj chaw;
Tau tshaj nrog cov tshuab Fluorescence PCR tseem ceeb;
Cov xwm txheej hloov pauv tau yooj yim rau cov tsev kho mob, CDCs thiab cov chaw kuaj mob;
Cov Khoom Siv Kuaj Xyuas Kab Mob Orthopox Hom Thoob Plaws / Kab Mob Monkeypox Nucleic Acid (Fluorescence PCR)
Kev them tag nrho: kuaj tag nrho 4 tus kab mob orthopox uas tuaj yeem kis rau tib neeg thiab cov kab mob mpox uas kis tau ( suav nrog Clade I & II) hauv ib qho kev kuaj kom tsis txhob pom tsis tau;
Muaj kev rhiab heev nrog LoD ntawm 200 daim / mL;
Muaj kev tshwj xeeb siab yam tsis muaj kev cuam tshuam nrog lwm cov kab mob ua rau muaj pob khaus xws li tus kab mob herpes simplex, tus kab mob varicella-zoster, thiab tib neeg genome, thiab lwm yam;
Kev ua haujlwm yooj yim: kev lysis qauv sai los ntawm cov qauv tso tawm reagent kom ntxiv rau hauv ib lub raj tshuaj tiv thaiv buffer;
Kev kuaj pom sai: kev nthuav dav sai nrog cov txiaj ntsig hauv 40 feeb;
Kev tseeb raug xyuas los ntawm kev tswj hwm sab hauv uas saib xyuas tag nrho cov txheej txheem kuaj pom;
Tau tshaj nrog cov tshuab Fluorescence PCR tseem ceeb;
Cov xwm txheej hloov pauv tau yooj yim rau cov tsev kho mob, CDCs thiab cov chaw kuaj mob;
Kab mob pob liabVirus TypingNucleicAcidDkev tshawb nrhiavKnws (FPCR kev ntsuas luminescence)
Txheeb xyuas Clade I thiab Clade II tib lub sijhawm, uas yog qhov tseem ceeb rau kev nkag siab txog cov yam ntxwv ntawm tus kab mob, taug qab nws txoj kev sib kis, thiab tsim cov kev tiv thaiv thiab tswj hwm.
Muaj kev rhiab heev nrog LoD ntawm 200 daim / mL;
Kev kuaj cov kua dej ua pob khaus, qhov swab oropharyngeal thiab serum;
Muaj qhov tshwj xeeb siab yam tsis muaj kev cuam tshuam ntawm Clade I thiab II, lwm yam kab mob ua rau muaj pob khaus xws li tus kab mob herpes simplex, tus kab mob varicella-zoster, thiab tib neeg genome, thiab lwm yam;
Kev ua haujlwm yooj yim: kev lysis qauv sai los ntawm cov qauv tso tawm reagent kom ntxiv rau hauv ib lub raj tshuaj tiv thaiv buffer;
Kev kuaj pom sai: tshwm sim hauv 40 feeb;
Kev tseeb raug xyuas los ntawm kev tswj hwm sab hauv uas saib xyuas tag nrho cov txheej txheem kuaj pom;
Lyophilized version tsuas yog xav tau kev thauj mus los thiab cia khoom hauv chav sov xwb ua rau nkag tau yooj yim rau txhua thaj chaw;
Tau tshaj nrog cov tshuab Fluorescence PCR tseem ceeb;
Cov xwm txheej hloov pauv tau yooj yim rau cov tsev kho mob, CDCs thiab cov chaw kuaj mob;
Kab Mob Liab Thoob Plaws Tag Nrho GenomeKev Tshawb PomKhoom siv (Ntau-PCR NGS)
Cov Khoom Siv Tshawb Nrhiav Kab Mob Monkeypox Tag Nrho Genome tshiab tsim los ntawm Macro & Micro-Test rau ntau qhov xwm txheej sib txawv, ua ke nrog ONT nanopore sequencer, tuaj yeem tau txais MPXV tag nrho cov kab ke genome nrog kev npog tsawg kawg 98% hauv 8 teev.
Yooj yim rau kev khiav lag luam: patented ib kauj ruam amplification technology, tag nrho cov genome sequence ntawm mpox tus kab mob tuaj yeem tau txais los ntawm ib-round amplification;
Rhiab thiab raug: ntes cov qauv qis rau 32CT, thiab 600bp amplicon nanopore sequencing tuaj yeem ua tau raws li kev sib dhos genome zoo dua;
Ceev heev: ONT tuaj yeem ua tiav kev sib sau ua ke ntawm genome hauv 6-8 teev;
Kev sib raug zoo dav dav: nrog ONT, Qi Carbon, SALUS Pro, lllumina, MGI thiab lwm yam khoom siv tseem ceeb 2ndthiab 3rdcov cim qhia txog tiam neeg.
rhiab heevKab mob liab tag nrho GenomeKev Tshawb PomKhoom siv-Illumina/MGI(Ntau-PCR NGS)
Txog cov lej loj ntawm 2 uas twb muaj lawmndcov cuab yeej tsim cov kab mob thoob ntiaj teb, Macro & Micro-Test kuj tau tsim cov khoom siv ultra-sensitive uas hloov kho rau cov cuab yeej sequencers tseem ceeb kom ua tiav cov qauv qis-concentration viral genome sequencing;
Kev ua kom nrov zoo: 1448 khub ntawm 200bp amplicon ultra-dense primer tsim rau kev ua kom nrov zoo thiab kev npog sib xws;
Kev ua haujlwm yooj yim: Mpox virus llumina/MGI tsev qiv ntawv tuaj yeem tau txais los ntawm kev ua kom loj dua ob zaug hauv 4 teev, zam cov kauj ruam tsim tsev qiv ntawv nyuaj thiab cov nqi reagent;
Kev rhiab heev: ntes cov qauv qis rau 35CT, zoo zam cov txiaj ntsig tsis zoo los ntawm kev puas tsuaj ntawm cov khoom tawg lossis tus lej theej qis;
Kev sib raug zoo dav nrog cov qauv tseem ceeb 2ndcov cuab yeej ua sequencers xws li lllumina, Salus Pro lossis MGI;Txog rau tam sim no, muaj ntau tshaj 400 rooj plaub kho mob tau ua tiav lawm.
Lub sijhawm tshaj tawm: Lub Yim Hli-28-2024