Cov Khoom Siv Tshawb Nrhiav Carbapenemases Uas Tshiab Tshaj Plaws

CRE, muaj nyob rau hauvkev pheej hmoo kis kab mob siab, kev tuag siab, tus nqi siab thiab nyuajhauv kev kho mob, hu rau ib qhokev tshawb nrhiav sai, ua haujlwm tau zoo thiab raugcov hau kev los pab kuaj mob thiab tswj hwm.

Raws li Kev Tshawb Fawb ntawm cov tsev kawm ntawv thiab cov tsev kho mob saum toj kawg nkaus, Cov Khoom Siv Tshawb Nrhiav Sai Sai Carbapenemases (Colloidal Gold) los ntawm Macro & Micro -Test, sib npaug rau ntau-qPCR assay, xa qhov tseeb 100% hauv kev txheeb xyuas carbapenemases hauv cov kab mob bacterial isolates. Qhov kev ua tau zoo tshaj qhov kev siv phenotypic ib txwm muaj xws li mCIM / eCIM thiab CDT. Qhov tseem ceeb, colloidal gold assays qhia txog 100% rhiab heev, qhov tshwj xeeb, qhov txiaj ntsig zoo, thiab qhov txiaj ntsig tsis zoo rau txhua qhov carbapenemase sim, qhia txog lawv qhov kev ua tau zoo thiab sib xws hauv kev txheeb xyuas cov kab mob carbapenemase tsim.

Kev Xaiv 1:

Sai saiCov Khoom Siv Tshawb Nrhiav Carbapenemases (Colloidal Gold), kev tshawb pom tshiab los daws qhov teeb meem ceev ceev ntawm CRE uas hem kev noj qab haus huv thoob ntiaj teb, 1-2 hnub ua ntej txoj kev siv tshuaj yeeb;

 15 feebtsuas yog txheeb xyuas NDM, KPC, OXA-48, IMP thiab VIM hauv ib qho kev sim xwb;

 Kev ua haujlwm yooj yim los ntawm cov kua ntshav tsis muaj phaj kab lis kev cai, 10 feeb tsuas yog rau lysis thiab ntxuav;

 Muaj kev rhiab heev & thiab tsis muaj kev cuam tshuam nrog cov kab mob pathogenic xws li Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa lossis lwm yam qauv β-lactamase;

 Kev Siv Tau Dav: Ntau yam xwm txheej suav nrog tsev kho mob, chaw kho mob, thiab chaw kho mob hauv zej zog.

Kev Xaiv 2:

Cov Khoom Siv Tshawb Nrhiav Gene Carbapenem Tiv Thaiv (Fluorescence PCR), Kev xeem 6-hauv-1nrog rau qhov tshwm sim hauv40 feeb, txheeb xyuas kom meejNDM, KPC, OXA23, OXA-48, IMP thiab VIMhauv ib qho kev xeem;

 Kev Kuaj Yooj Yim: Cov Hnoos, daim ntaub so quav lossis cov kab mob huv si;

 Txo Tus Nqi: 6 lub hom phiaj uas pom los ntawm 1 qhov kev sim ib zaug zam kev sim ntau dhau;

 Kev rhiab heev thiab kev tshwj xeeb: 1000CFU / mL rau kev rhiab heev thiab tsis muaj kev cuam tshuam nrog lwm cov kab mob ua pa lossis cov qauv uas muaj lwm cov noob tiv taus tshuaj CTX, mecA, SME, SHV, TEM, thiab lwm yam.

 Kev Sib Tw Dav Dav: Nrog cov cuab yeej PCR tseem ceeb;