Ib Lub Riam Tshiab rau Kev Tshawb Fawb Tuberculosis thiab Kev Tshawb Nrhiav Tshuaj Tiv Thaiv: Ib Yam Tshiab Lub Hom Phiaj Sequencing (tNGS) Ua ke nrog Tshuab Kawm rau Tuberculosis Hypersensitivity Diagnosis
Daim ntawv ceeb toom: CCa: tus qauv kuaj mob raws li tNGS thiab kev kawm tshuab, uas tsim nyog rau cov neeg uas muaj kab mob me me thiab kab mob ntsws ntsws.
Thesis title: Tuberculous-targeted next-generation sequencing and machine learning: ib tug ultra-sensitive diagnostic zoo tswv yim rau paucific pulmonary tubulars thiab tubular meningitis.
Periodical: 'Clinica Chimica Acta'
Ib: 6.5
Hnub luam tawm: Lub Ib Hlis 2024
Ua ke nrog University of Suav Academy ntawm Sciences thiab Beijing Chest Tsev Kho Mob ntawm Capital Medical University, Macro & Micro-Test tsim ib tug qauv kuaj tuberculosis raws li cov tiam tshiab ntawm lub hom phiaj sequencing (tNGS) technology thiab tshuab kawm txoj kev, uas muab ultra-siab rhiab heev rau tuberculosis nrog ob peb cov kab mob thiab tuberculous meningitis, muab ib tug tshiab hypersensitivity diagnosis txoj kev. ntawm tuberculosis, thiab pab qhov kev kuaj mob kom raug, kev tshawb nrhiav tshuaj tiv thaiv thiab kho tuberculosis. Nyob rau tib lub sijhawm, nws pom tau tias tus neeg mob lub plasma cfDNA tuaj yeem siv los ua ib hom qauv tsim nyog rau kev kuaj mob hauv kev kuaj mob TBM.
Nyob rau hauv txoj kev tshawb no, 227 cov qauv ntshav thiab cov kua cerebrospinal kuaj tau siv los tsim ob qhov chaw kho mob, uas lub chaw kuaj mob kuaj mob tau siv los tsim cov qauv kev kawm tshuab ntawm kev kuaj mob tuberculosis, thiab cov qauv kuaj mob kuaj mob tau siv los txheeb xyuas cov qauv tsim. Tag nrho cov qauv yog thawj zaug tsom los ntawm kev tsim tshwj xeeb uas tsim los soj ntsuam lub pas dej rau Mycobacterium tuberculosis. Tom qab ntawd, raws li cov ntaub ntawv TB-tNGS sequencing, cov qauv txiav txim siab yog siv los ua 5-fold cross-validation ntawm cov kev cob qhia thiab kev siv tau ntawm cov chav kuaj kuaj kab mob, thiab kev kuaj ntshav kuaj ntshav thiab kuaj ntshav cerebrospinal. Cov theem pib tau txais yog coj mus rau hauv ob qhov kev sim ntawm cov kab mob kuaj mob rau kev kuaj pom, thiab kev kuaj mob ntawm tus neeg kawm tau raug ntsuas los ntawm ROC nkhaus. Thaum kawg, tus qauv kuaj mob ntawm tuberculosis tau txais.
Daim duab 1 schematic daim duab ntawm kev tshawb fawb tsim
Cov txiaj ntsig: Raws li qhov tshwj xeeb ntawm CSF DNA qauv (AUC = 0.974) thiab plasma cfDNA qauv (AUC = 0.908) tau txiav txim siab hauv txoj kev tshawb no, ntawm 227 tus qauv, qhov rhiab heev ntawm CSF tus qauv yog 97.01%, qhov tshwj xeeb yog 95.65%, thiab qhov tshwj xeeb plasma 1.8% thiab rhiab heev. 86.36%. Hauv kev tshuaj xyuas ntawm 44 cov qauv ua ke ntawm plasma cfDNA thiab cerebrospinal kua DNA los ntawm cov neeg mob TBM, lub tswv yim kev kuaj mob ntawm txoj kev tshawb no muaj qhov sib xws ntawm 90.91% (40/44) hauv cov ntshav cfDNA thiab cerebrospinal kua DNA, thiab qhov rhiab heev yog 95.45% (95.45%). Hauv cov menyuam yaus uas muaj tus kab mob ntsws ntsws, qhov kev kuaj mob ntawm txoj kev tshawb no muaj kev nkag siab ntau dua rau cov qauv ntshav plasma ntau dua li Xpert kuaj pom cov kua dej hauv plab los ntawm tib cov neeg mob (28.57% VS 15.38%).
Fig. 2 Kev soj ntsuam kev ua tau zoo ntawm kev kuaj mob tuberculosis qauv rau cov qauv pej xeem
Fig. 3 Diagnostic results of paired samples
Xaus: Ib txoj kev kuaj mob siab rau tuberculosis tau tsim nyob rau hauv txoj kev tshawb no, uas tuaj yeem muab cov cuab yeej kuaj mob nrog kev tshawb pom siab tshaj plaws rau cov neeg mob uas muaj oligobacillary tuberculosis (kev coj noj coj ua tsis zoo). Kev kuaj pom tus kab mob siab ntsws raws li plasma cfDNA tej zaum yuav yog ib hom qauv tsim nyog rau kev kuaj mob ntawm cov kab mob ntsws thiab mob ntsws ntsws (plasma kuaj tau yooj yim sau dua li cov kua dej cerebrospinal rau cov neeg mob uas xav tias mob ntsws ntsws).
Original link: https://www.sciencedirect.com/science/article/pii/s0009898123004990? ntawm %3Dihub
Kev taw qhia luv luv ntawm Macro & Micro-Test tuberculosis series nrhiav cov khoom
Nyob rau hauv qhov kev pom ntawm cov qauv nyuaj ntawm cov neeg mob tuberculosis thiab ntau yam kev xav tau, Macro & Micro-Test muab cov txheej txheem tiav ntawm NGS cov kev daws teeb meem rau liquefaction extraction los ntawm cov hnoos qeev, Qualcomm tsev qiv ntawv kev tsim kho thiab kev sib txuas, thiab kev tshuaj xyuas cov ntaub ntawv. Cov khoom lag luam suav nrog kev kuaj mob sai sai ntawm cov neeg mob tuberculosis, tshuaj tiv thaiv kev kuaj mob ntawm tuberculosis, ntaus ntawv ntawm mycobacterium tuberculosis thiab NTM, kev kuaj pom tsis zoo ntawm cov kab mob-tsis zoo tuberculosis thiab cov neeg tuberculous, thiab lwm yam.
Cov khoom siv kuaj kab mob rau tuberculosis thiab mycobacteria:
| Yam khoom No | npe khoom | cov khoom kuaj cov ntsiab lus | hom qauv | siv tau tus qauv |
| HWM-3012 | Qauv tso tawm tus neeg sawv cev | Siv rau hauv kev kho liquefaction ntawm cov hnoos qeev, tau txais cov ntaub ntawv thawj zaug, Sutong Machinery Equipment 20230047. | hnoos qeev | |
| HWTS-NGS-P00021 | Qualcomm cov khoom siv nrhiav kom muaj nuj nqis rau cov kab mob siab ntsws (kev sojntsuam ntes) | Non-invasive (kua biopsy) hypersensitivity detection rau cov kab mob-tsis zoo pulmonary tuberculosis thiab hlwb nodules; Cov qauv ntawm cov neeg uas xav tias yuav kis tau tus kab mob tuberculosis los yog tsis-tuberculosis mycobacteria tau soj ntsuam los ntawm qhov tob sib sib zog nqus metaagenomics, thiab cov ntaub ntawv tshawb nrhiav seb puas yog tuberculosis lossis non-tuberculosis mycobacteria tau kis thiab cov ntaub ntawv tseem ceeb thawj kab tshuaj tiv thaiv ntawm mycobacterium tuberculosis tau muab. | Peripheral ntshav, alveolar lavage kua, hydrothorax thiab ascites, tsom puncture qauv, cerebrospinal kua. | tiam thib ob |
| HWTS-NGS-T001 | Mycobacterium typing thiab drug resistance detection kit (multiplex amplification sequencing method) | Mycobacterium typing test, suav nrog MTBC thiab 187 NTM.Kev tshawb nrhiav tshuaj tiv thaiv ntawm Mycobacterium tuberculosis npog 13 cov tshuaj thiab 16 qhov chaw hloov pauv ntawm cov tshuaj tiv thaiv cov noob. | Sputum, alveolar lavage kua, hydrothorax thiab ascites, tsom puncture qauv, cerebrospinal kua. | Thib ob / thib peb tiam dual platform |
Cov ntsiab lus tseem ceeb: HWTS-NGS-T001 Mycobacterium typing and drug resistance detection kit (multiplex amplification method)
Khoom taw qhia
Cov khoom lag luam yog raws li cov tshuaj tseem ceeb thawj thiab thib ob uas tau piav qhia hauv WHO cov lus qhia kho TB, macrolides thiab aminoglycosides feem ntau siv hauv NTM cov txheej txheem kho mob, thiab cov chaw tshuaj tiv thaiv npog tag nrho ib pawg ntawm cov tshuaj tiv thaiv qhov chaw nyob hauv WHO Mycobacterium tuberculosis complex mutation catalogue, nrog rau lwm cov ntaub ntawv qhia txog cov tshuaj tiv thaiv kab mob thiab cov tshuaj tiv thaiv kab mob raws li kev txheeb cais. cov ntaub ntawv hauv tsev thiab txawv teb chaws.
Kev txheeb xyuas kev ntaus ntawv yog ua raws li NTM cov kab mob uas tau sau tseg hauv NTM cov lus qhia luam tawm los ntawm Suav Phau Ntawv Xov Xwm ntawm Tuberculosis thiab Kab Mob Ua Pa thiab kev pom zoo ntawm cov kws tshaj lij. Tus tsim cov ntawv ntaus ntawv tuaj yeem nthuav dav, ua ntu zus thiab sau ntau tshaj 190 NTM hom.
Los ntawm lub hom phiaj multiplex PCR amplification technology, genotyping genes thiab tshuaj-resistant genes ntawm Mycobacterium tau amplified los ntawm multiplex PCR, thiab amplicon ua ke ntawm lub hom phiaj noob tau kuaj tau. Cov khoom lag luam amplified tuaj yeem tsim tau rau hauv tiam thib ob lossis thib peb-tiam high-throughput sequencing qiv, thiab tag nrho cov tiam thib ob thiab thib peb-tiam sequencing platforms yuav raug rau high-depth sequencing kom tau txais cov ntaub ntawv sib lawv liag ntawm cov hom phiaj. Los ntawm kev sib piv nrog cov kev hloov pauv uas paub muaj nyob rau hauv cov ntaub ntawv siv hauv built-in (xws li WHO Mycobacterium tuberculosis complex mutation catalog thiab nws txoj kev sib raug zoo nrog cov tshuaj tiv thaiv), cov kev hloov pauv cuam tshuam txog tshuaj tiv thaiv lossis kev cuam tshuam ntawm cov tshuaj tiv thaiv kab mob tau txiav txim siab. Ua ke nrog tus kheej qhib cov hnoos qeev cov qauv kev kho mob ntawm Macro & Micro-Test, qhov teeb meem ntawm cov nucleic acid amplification efficiency ntawm cov hnoos qeev qis (tsawg zaus siab dua li cov txheej txheem ib txwm siv) tau daws, kom cov tshuaj tiv thaiv sequencing nrhiav tau ncaj qha rau kev kuaj hnoos qeev.
Cov khoom nrhiav pom ntau yam
34tshuaj tiv thaiv kab mob ntsig txog cov noob ntawm18tshuaj tiv thaiv tuberculosis thiab6NTM tshuaj raug kuaj pom, npog297 ibcov chaw tiv thaiv tshuaj; Kaum hom Mycobacterium tuberculosis thiab ntau dua190tau kuaj pom muaj hom NTM.
Table 1: Cov ntaub ntawv ntawm 18+6 Tshuaj +190+NTM
Khoom kom zoo dua
Kev kho kom haum xeeb muaj zog: cov hnoos qeev tuaj yeem kuaj pom ncaj qha nrog tus neeg sawv cev liquefaction yam tsis muaj kab lis kev cai.
Kev sim ua haujlwm yog yooj yim: thawj zaug ntawm kev ua haujlwm amplification yog qhov yooj yim, thiab kev tsim kho tsev qiv ntawv tau ua tiav hauv 3 teev, uas txhim kho kev ua haujlwm zoo.
Kev ntaus ntawv thiab kev siv tshuaj tiv thaiv: npog qhov chaw ntaus ntawv thiab tshuaj tiv thaiv ntawm MTB thiab NTM, uas yog cov ntsiab lus tseem ceeb ntawm kev txhawj xeeb hauv kev kho mob, kev ntaus ntawv raug thiab tshuaj tiv thaiv tshuaj tiv thaiv, txhawb kev ywj pheej tshawb xyuas software, thiab tsim cov ntaub ntawv txheeb xyuas nrog ib nias.
Compatibility: khoom compatibility, adapting rau mainstream ILM thiab MGI/ONT platforms.
Khoom specification
| Khoom code | npe khoom | Tshawb nrhiav platform | specifications |
| HWTS-NGS-T001 | Mycobacterium typing thiab drug resistance detection kit (multiplex amplification method) | ONT, Illumina, MGI, Salus pro | 16/96 r ib |
Post lub sij hawm: Jan-23-2024