Ib Lub Riam Tshiab rau Kev Tshawb Fawb Tuberculosis thiab Kev Tshawb Nrhiav Tshuaj Tiv Thaiv: Ib Tug Tshiab Lub Hom Phiaj Sequencing (tNGS) Ua ke nrog Tshuab Kawm rau Tuberculosis Hypersensitivity Diagnosis
Daim ntawv ceeb toom: CCa: tus qauv kuaj mob raws li tNGS thiab kev kawm tshuab, uas tsim nyog rau cov neeg uas muaj kab mob me me thiab kab mob ntsws ntsws.
Thesis title: Tuberculous-targeted next-generation sequencing and machine learning: ib tug ultra-sensitive diagnostic zoo tswv yim rau paucific pulmonary tubulars thiab tubular meningitis.
Periodical: 'Clinica Chimica Acta'
Ib: 6.5
Hnub luam tawm: Lub Ib Hlis 2024
Ua ke nrog University of Suav Academy ntawm Sciences thiab Beijing Chest Tsev Kho Mob ntawm Capital Medical University, Macro & Micro-Test tsim ib tug qauv kuaj tuberculosis raws li cov tiam tshiab ntawm tsom sequencing (tNGS) technology thiab tshuab kev kawm txujci, uas muab ultra-siab. Kev tshawb nrhiav rhiab heev rau tuberculosis nrog ob peb kab mob thiab tuberculous meningitis, muab txoj kev kuaj mob ntshav siab tshiab rau kev kuaj mob ntawm ob hom tuberculosis, thiab pab kev kuaj mob kom raug, kev tshawb nrhiav tshuaj tiv thaiv thiab kho tuberculosis.Nyob rau tib lub sijhawm, nws pom tau tias tus neeg mob lub plasma cfDNA tuaj yeem siv los ua cov qauv tsim nyog rau kev kuaj mob hauv kev kuaj mob TBM.
Nyob rau hauv txoj kev tshawb no, 227 cov qauv ntshav thiab cov kua cerebrospinal kuaj tau siv los tsim ob qhov chaw kho mob, uas cov qauv kuaj kuaj mob tau siv los tsim cov qauv kev kawm tshuab ntawm kev kuaj mob tuberculosis, thiab cov qauv kuaj mob kuaj mob tau siv los txheeb xyuas cov qauv tsim. kuaj qauv.Tag nrho cov qauv yog thawj zaug tsom los ntawm kev tsim tshwj xeeb uas tsim los soj ntsuam lub pas dej rau Mycobacterium tuberculosis.Tom qab ntawd, raws li cov ntaub ntawv TB-tNGS sequencing, cov qauv txiav txim siab yog siv los ua 5-fold cross-validation ntawm cov kev cob qhia thiab kev siv tau ntawm cov chav kuaj kuaj kab mob, thiab kev kuaj ntshav kuaj ntshav thiab kuaj ntshav cerebrospinal.Cov theem pib tau txais yog coj mus rau hauv ob qhov kev sim ntawm cov kab mob kuaj mob rau kev kuaj pom, thiab kev kuaj mob ntawm tus neeg kawm tau raug ntsuas los ntawm ROC nkhaus.Thaum kawg, tus qauv kuaj mob ntawm tuberculosis tau txais.
Daim duab 1 schematic daim duab ntawm kev tshawb fawb tsim
Cov txiaj ntsig: Raws li qhov tshwj xeeb ntawm CSF DNA qauv (AUC = 0.974) thiab plasma cfDNA qauv (AUC = 0.908) tau txiav txim siab hauv txoj kev tshawb no, ntawm 227 tus qauv, qhov rhiab heev ntawm CSF qauv yog 97.01%, qhov tshwj xeeb yog 95.65%, thiab 82.61% thiab 86.36%.Hauv kev tshuaj xyuas ntawm 44 cov qauv ua ke ntawm plasma cfDNA thiab cerebrospinal kua DNA los ntawm cov neeg mob TBM, lub tswv yim kuaj mob ntawm txoj kev tshawb no muaj qhov sib xws ntawm 90.91% (40/44) hauv plasma cfDNA thiab cerebrospinal kua DNA, thiab qhov rhiab heev yog 95.45%. (42/44).Hauv cov menyuam yaus uas muaj tus kab mob ntsws ntsws, qhov kev kuaj mob ntawm txoj kev tshawb no muaj kev nkag siab ntau dua rau cov qauv ntshav plasma ntau dua li Xpert kuaj pom cov kua dej hauv plab los ntawm tib cov neeg mob (28.57% VS 15.38%).
Fig. 2 Kev soj ntsuam kev ua tau zoo ntawm kev kuaj mob tuberculosis qauv rau cov qauv pej xeem
Fig. 3 Diagnostic results of paired samples
Xaus: Ib txoj kev kuaj mob siab rau tuberculosis tau tsim nyob rau hauv txoj kev tshawb no, uas tuaj yeem muab cov cuab yeej kuaj mob nrog kev kuaj pom siab tshaj plaws rau cov neeg mob uas muaj oligobacillary tuberculosis (kev coj noj coj ua tsis zoo).Kev kuaj pom tus kab mob hypersensitive tuberculosis raws li plasma cfDNA tej zaum yuav yog ib hom qauv tsim nyog rau kev kuaj mob ntawm tus kab mob ntsws thiab mob ntsws ntsws (plasma kuaj tau yooj yim los sau dua li cov kua dej hauv cerebrospinal rau cov neeg mob uas xav tias mob ntsws ntsws).
Original link: https://www.sciencedirect.com/science/article/pii/s0009898123004990?ntawm %3Dihub
Kev taw qhia luv luv ntawm Macro & Micro-Test tuberculosis series nrhiav cov khoom
Nyob rau hauv qhov kev pom ntawm cov qauv nyuaj ntawm cov neeg mob tuberculosis thiab ntau yam kev xav tau, Macro & Micro-Test muab cov txheej txheem tiav ntawm NGS cov kev daws teeb meem rau liquefaction extraction los ntawm cov hnoos qeev, Qualcomm tsev qiv ntawv kev tsim kho thiab kev sib txuas, thiab kev tshuaj xyuas cov ntaub ntawv.Cov khoom lag luam suav nrog kev kuaj mob sai sai ntawm cov neeg mob tuberculosis, tshuaj tiv thaiv kev kuaj mob ntawm tuberculosis, ntaus ntawv ntawm mycobacterium tuberculosis thiab NTM, kev kuaj pom tsis zoo ntawm cov kab mob-tsis zoo tuberculosis thiab cov neeg tuberculous, thiab lwm yam.
Cov khoom siv kuaj kab mob rau tuberculosis thiab mycobacteria:
| Yam khoom No | npe khoom | cov khoom kuaj cov ntsiab lus | hom qauv | siv tau tus qauv |
| HWM-3012 | Qauv tso tawm tus neeg sawv cev | Siv rau hauv kev kho liquefaction ntawm cov hnoos qeev, tau txais cov ntaub ntawv thawj zaug, Sutong Machinery Equipment 20230047. | hnoos qeev | |
| HWTS-NGS-P00021 | Qualcomm cov khoom siv nrhiav kom muaj nuj nqis rau cov kab mob siab ntsws (kev sojntsuam ntes) | Non-invasive (kua biopsy) hypersensitivity detection rau cov kab mob-tsis zoo pulmonary tuberculosis thiab hlwb nodules;Cov qauv ntawm cov neeg uas xav tias yuav kis tau tus kab mob los yog tsis-tuberculosis mycobacteria tau soj ntsuam los ntawm qhov tob sib sib zog nqus metagenomics, thiab cov ntaub ntawv tshawb nrhiav seb puas yog tuberculosis lossis non-tuberculosis mycobacteria tau kis thiab cov ntaub ntawv tseem ceeb thawj kab tshuaj tiv thaiv ntawm mycobacterium tuberculosis. tau muab. | Peripheral ntshav, alveolar lavage kua, hydrothorax thiab ascites, tsom puncture qauv, cerebrospinal kua. | tiam thib ob |
| HWTS-NGS-T001 | Mycobacterium typing thiab drug resistance detection kit (multiplex amplification sequencing method) | Mycobacterium typing test, suav nrog MTBC thiab 187 NTM.Kev tshawb nrhiav tshuaj tiv thaiv ntawm Mycobacterium tuberculosis npog 13 cov tshuaj thiab 16 qhov chaw hloov pauv ntawm cov tshuaj tiv thaiv cov noob. | Sputum, alveolar lavage kua, hydrothorax thiab ascites, tsom puncture qauv, cerebrospinal kua. | Thib ob / thib peb tiam dual platform |
Cov ntsiab lus tseem ceeb: HWTS-NGS-T001 Mycobacterium typing and drug resistance detection kit (multiplex amplification method)
Khoom taw qhia
Cov khoom lag luam yog ua raws li cov tshuaj tseem ceeb thawj thiab thib ob uas tau piav qhia hauv WHO cov lus qhia txog kev kho mob TB, macrolides thiab aminoglycosides feem ntau siv hauv NTM cov txheej txheem kho mob, thiab cov chaw tshuaj tiv thaiv npog tag nrho ib pawg ntawm cov tshuaj tiv thaiv cov chaw nyob hauv WHO Mycobacterium tuberculosis complex mutation catalogue, nrog rau lwm cov tshuaj tiv thaiv kab mob thiab cov chaw hloov pauv raws li kev tshawb nrhiav thiab kev txheeb cais ntawm cov ntaub ntawv tau qhab nia siab hauv tsev thiab txawv teb chaws.
Kev txheeb xyuas kev ntaus ntawv yog ua raws li NTM cov kab mob uas tau sau tseg hauv NTM cov lus qhia luam tawm los ntawm Suav Phau Ntawv Xov Xwm ntawm Tuberculosis thiab Kab Mob Ua Pa thiab kev pom zoo ntawm cov kws tshaj lij.Tus tsim cov ntawv ntaus ntawv tuaj yeem nthuav dav, ua ntu zus thiab sau ntau tshaj 190 NTM hom.
Los ntawm lub hom phiaj multiplex PCR amplification technology, genotyping genes thiab tshuaj-resistant genes ntawm Mycobacterium tau amplified los ntawm multiplex PCR, thiab amplicon ua ke ntawm lub hom phiaj noob tau kuaj tau.Cov khoom lag luam amplified tuaj yeem tsim tau rau hauv tiam thib ob lossis thib peb tiam high-throughput sequencing qiv, thiab tag nrho cov tiam thib ob thiab thib peb-tiam sequencing platforms tuaj yeem ua rau qhov sib sib zog nqus tob kom tau txais cov ntaub ntawv sib txuas ntawm cov hom phiaj.Los ntawm kev sib piv nrog cov kev hloov pauv uas paub muaj nyob rau hauv cov ntaub ntawv siv hauv built-in (xws li WHO Mycobacterium tuberculosis complex mutation catalog thiab nws txoj kev sib raug zoo nrog cov tshuaj tiv thaiv), cov kev hloov pauv cuam tshuam txog tshuaj tiv thaiv lossis kev cuam tshuam ntawm cov tshuaj tiv thaiv kab mob tau txiav txim siab.Ua ke nrog tus kheej qhib cov hnoos qeev cov qauv kev kho mob ntawm Macro & Micro-Test, qhov teeb meem ntawm qhov tsis tshua muaj nucleic acid amplification efficiency ntawm cov tshuaj hnoos qeev ( kaum npaug siab dua li ntawm cov txheej txheem ib txwm siv) tau daws, kom cov tshuaj tiv thaiv sequencing nrhiav tau. ncaj qha mus rau kev kuaj mob hnoos qeev.
Cov khoom nrhiav pom ntau yam
34tshuaj tiv thaiv kab mob ntsig txog cov noob ntawm18tshuaj tiv thaiv tuberculosis thiab6NTM tshuaj raug kuaj pom, npog297 ibcov chaw tiv thaiv tshuaj;Kaum hom Mycobacterium tuberculosis thiab ntau dua190nrhiav tau hom NTM.
Table 1: Cov ntaub ntawv ntawm 18+6 Tshuaj +190+NTM
Khoom kom zoo dua
Kev kho kom haum xeeb muaj zog: cov hnoos qeev tuaj yeem kuaj pom ncaj qha nrog tus kheej liquefaction tus neeg saib xyuas yam tsis muaj kab lis kev cai.
Kev sim ua haujlwm yog qhov yooj yim: thawj zaug ntawm kev ua haujlwm amplification yog qhov yooj yim, thiab kev tsim kho tsev qiv ntawv tiav hauv 3 teev, uas txhim kho kev ua haujlwm zoo.
Kev ntaus ntawv thiab kev siv tshuaj tiv thaiv: npog qhov chaw ntaus ntawv thiab tshuaj tiv thaiv ntawm MTB thiab NTM, uas yog cov ntsiab lus tseem ceeb ntawm kev txhawj xeeb hauv kev kho mob, kev ntaus ntawv raug thiab tshuaj tiv thaiv tshuaj tiv thaiv, txhawb kev ywj pheej tshawb xyuas software, thiab tsim cov ntaub ntawv txheeb xyuas nrog ib nias.
Compatibility: khoom compatibility, adapting rau mainstream ILM thiab MGI/ONT platforms.
Khoom specification
| Khoom code | npe khoom | Tshawb nrhiav platform | specifications |
| HWTS-NGS-T001 | Mycobacterium typing thiab drug resistance detection kit (multiplex amplification method) | ONT, Illumina, MGI, Salus pro | 16/96 r ib |
Post lub sij hawm: Jan-23-2024