Thaum kev kuaj nucleic acid dhau los ua qhov tsim nyog niaj hnub, koj puas tau ntsib cov teeb meem no: cov tshuaj reagents uas muaj kev pheej hmoo ntawm kev puas tsuaj thaum thauj mus los, cov txheej txheem qhib uas yooj yim ua rau muaj kuab paug, lossis kev poob haujlwm los ntawm kev rov ua dua ntawm cov voj voog khov-thaw?
Ib lub tshuab thev naus laus zis "ua siab tawv" uas muaj hnub nyoog ntau pua xyoo -kev ziab kom qhuav hauv lub tshuab nqus tsev—tam sim no tab tom tsav tsheb hloov pauv tshiab hauv kev lag luam kuaj mob molecular.
Niaj hnub no, cia peb tshawb nrhiav seb yuav ua li casMacroMicroTech (MMT)tab tom daws plaub qhov teeb meem loj nrog nwssiv ib zaug xwb, ua tiav cov khoom khov-qhuavlub microspherethev naus laus zis.
I. Kev Rov Qab Los Zoo Kawg Nkaus ntawm Kev Siv Tshuab Ib Xyoos Dhau Los: Keeb Kwm Luv Luv ntawm Kev Khov-Qhuav
Xyoo 1909, cov kws tshawb fawb thawj zaug siv kev ziab kom qhuav los khaws cov serum; los ntawm xyoo 1919, nws ua rau khaws cov kab mob viral tau ntev. Yav tas los hu ua "tus saib xyuas bioactivity," kev ziab kom qhuav tam sim no tau rov ua dua tshiab thiab siv dav hauv kev kuaj mob molecular niaj hnub no.
1. Kev Mus Ncig Ua Si Nyuaj Siab ntawm Cov Tshuaj Reagents Ib Txwm Muaj
Txij ntawm cov chaw tsim khoom mus rau cov neeg siv kawg, cov kua tshuaj reagents yuav tsum tau khaws cia rau hauv qhov chaw txias nruj thoob plaws hauv kev khaws cia thiab kev thauj mus los. Kev siv tsis raug feem ntau ua rau cov enzyme puas tsuaj, tsis ruaj khov ntawm lub probe, thiab ua tsis tiav cov tshuaj tiv thaiv - qhov teeb meem rov tshwm sim tau sau tseg hauv cov kev cob qhia thiab cov ntaub ntawv tshawb fawb.
2. Lub Neej Muaj Kev Pheej Hmoo Siab ntawm Cov Tshuaj Reagents Ua Kua
Thaum qhib lawm, ib lub thawv uas muaj cov tshuaj reagents khov feem ntau yuav siv sijhawm ntau lub voj voog ntawm kev yaj, siv, thiab rov khov dua—kom txog thaum noj tag lossis pov tseg—ua rau poob haujlwm thiab pov tseg.
Kev ziab khov daws cov teeb meem no ntawm cov hauv paus. Los ntawm kev tshem tawm cov dej hauv qab lub tshuab nqus tsev ntawm qhov kub qis, cov enzymes thiab cov probes raug "xauv" rau hauv qhov xwm txheej ruaj khov - qhov tseem ceeb yog nias ib ntus thaum lub sijhawm lwj mus txog thaum siv.
II. Plaub Qhov Zoo Tseem Ceeb: Vim Li Cas Kev Khov Kom Qhuav Yog Qhov Kev daws teeb meem zoo tshaj plaws rau Kev Tshuaj Xyuas Molecular
MMT cov microspheres uas siv ib zaug xwb, uas tau tsim los kom qhuav tag nrho yog tsim los tshem tawm cov teeb meem tseem ceeb ntawm kev lag luam:
III. Kev Tshawb Fawb Txog Tshuab: Vim Li Cas MMT Thiaj Xaiv Freeze-Dried Microlub voj voogs?
Ntawm ntau hom xws li kev siv tshuaj lyophilization hauv lub raj mis lossis PCR-tube, MMT tau tsim tsa ib qhomicro-qhuav qhuavlub voj vooghom ntawvuas ua tiav peb qhov kev tshawb pom tseem ceeb:
koj"Ib tugLub microsphere= Ib Qho Kev Tshwm Sim Tag Nrho
Txhua 2 hli Microsphere muaj tag nrho cov khoom
—cov tshuaj primers, cov enzymes, dNTPs—ua kom muaj qhov tseeb thiab tshem tawm cov yuam kev aliquoting.
kojKev Kho Dua Tshiab 1-Feeb
Cov khoom siv tshwj xeeb tso cai rau cov microspheres yaj sai dua 50% dua li cov qauv khov-qhuav ib txwm muaj.
kojKev Sib Txuas Lus Qhib-Hom Ntawv
Siv tau nrog ntau yam khoom siv thiab cov khoom siv (piv txwv li, 8-strip PCR raj, hom ntawv siv ib zaug), kom ntseeg tau tias muaj kev ywj pheej ntawm kev siv.
IV. Yav Tom Ntej Yog Tam Sim No: Yuav Ua Li Cas Freeze-Drying Yuav Hloov Kho Kev Kuaj Mob Molecular
Thaum kev kuaj mob molecular txav mus ze rau qhov chaw saib xyuas neeg mob, kev kuaj mob hauv tsev, thiab kev kho mob thawj zaug, MMT tau npaj txhij nrog nws lub platform khov-qhuav uas muaj zog.
Piv txwv li, hauv kev sim vaj huam sib luag ua pa, cov microspheres uas khov-qhuav muab cov txiaj ntsig txhim khu kev qha nrog kev yooj yim, tsis muaj saw hlau txias lossis kev teeb tsa nyuaj.
Cov Txheej Txheem Ib Txwm Muaj:
Rau AIO800
Los ntawm"kev ntxhov siab txog saw hlau txias"mus rau"kev ywj pheej ntawm qhov kub thiab txias hauv chav,"los ntawm"cov txheej txheem nyuaj"mus rau"ib kauj ruam nrhiav pom," thev naus laus zis ua kom qhuav khov kho tau txhais dua tshiab txog cov ciam teb ntawm kev ntseeg tau hauv kev kuaj mob molecular.
MMT yuav txuas ntxiv tsim kho tshiab raws txoj kev ruaj khov, ua haujlwm tau zoo, yooj yim, thiab yooj yim—ua kom muaj zog rau kev kuaj mob kom raug kom dim ntawm kev txwv sijhawm thiab qhov chaw.
Lub sijhawm tshaj tawm: Lub Kaum Ob Hlis-26-2025