Clostridium difficile toxin A/B noob (C.diff)

Lus piav qhia luv luv:

Cov khoom siv no yog tsim los rau kev tshawb nrhiav hauv vitro zoo ntawm clostridium difficile toxin A gene thiab toxin B gene hauv cov quav kuaj los ntawm cov neeg mob uas xav tias muaj kab mob clostridium difficile.


Product Detail

Khoom cim npe

Khoom npe

HWTS-OT031A Nucleic Acid Detection Kit rau Clostridium difficile toxin A/B noob (C.diff) (Fluorescence PCR)

Daim ntawv pov thawj

CE

Kab mob sib kis

Clostridium difficile (CD), gram-zoo anaerobic sporogenic Clostridium difficile, yog ib qho ntawm cov kab mob tseem ceeb ua rau cov kab mob nosocomial plab hnyuv.Clinically, kwv yees li 15% ~ 25% ntawm cov tshuaj tiv thaiv kab mob ntsig txog kab mob, 50% ~ 75% ntawm cov tshuaj tiv thaiv kab mob sib kis thiab 95% ~ 100% ntawm pseudomembranous enteritis yog tshwm sim los ntawm Clostridium difficile infection (CDI).Clostridium difficile yog ib hom kab mob, nrog rau cov kab mob toxigenic thiab cov kab mob uas tsis yog toxigenic.

Channel

FAM tcdAnoob
ROX tcdBnoob
VIC/HEX Internal Control

Technical Parameters

Cia

≤-18 ℃

Txee lub neej 12 hli
Hom Qauv quav
Tt ≤38
CV ≤ 5.0%
LoD 200 CFU/ml
Qhov tshwj xeeb siv cov khoom siv no los kuaj xyuas lwm cov kab mob hauv plab hnyuv xws li Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza human virus, influenza A. DNA, cov txiaj ntsig yog txhua yam tsis zoo.
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Ua hauj lwm Flow

Kev xaiv 1.

Ntxiv 180μL ntawm lysozyme tsis nyob rau hauv precipitate (dilute lysozyme rau 20mg / mL nrog lysozyme diluent), pipette kom sib tov zoo, thiab txheej txheem ntawm 37 ° C rau ntau tshaj 30 feeb. Siv 1.5mL ntawm RNase / DNase-dawb centrifuge tube, thiab ntxiv180μL ntawm kev tswj zoo thiab kev tswj tsis zoo nyob rau hauv ib ntus.Ntxiv10μL ntawm kev tswj hwm sab hauv rau cov qauv kom raug kuaj, kev tswj zoo, thiab kev tswj tsis zoo hauv ntu, thiab siv Nucleic Acid Extraction lossis Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd. rau cov qauv DNA rho tawm tom ntej, thiab thov ua raws nraim li cov lus qhia siv rau cov kauj ruam tshwj xeeb.Siv DNase/RNase dawb H2O rau elution, thiab qhov pom zoo elution ntim yog 100μL.

Kev xaiv 2.

Noj 1.5mL ntawm RNase / DNase-dawb centrifuge raj, thiab ntxiv 200μL ntawm kev tswj zoo thiab kev tswj tsis zoo nyob rau hauv ib ntus.Ntxiv10μL ntawm kev tswj hwm sab hauv rau cov qauv kom raug kuaj, kev tswj zoo, thiab kev tswj tsis zoo hauv ntu, thiab siv Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv, thiab qhov ntim tau pom zoo yog 80μL.

 


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