Clostridium difficile toxin A/B gene (C.diff)
Lub npe khoom
HWTS-OT031A Nucleic Acid Detection Kit rau Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Daim Ntawv Pov Thawj
CE
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Clostridium difficile (CD), yog ib hom kab mob gram-positive anaerobic sporogenic Clostridium difficile, yog ib qho ntawm cov kab mob tseem ceeb ua rau muaj kev kis kab mob hauv plab hnyuv. Hauv kev kho mob, kwv yees li 15% ~ 25% ntawm kev raws plab uas cuam tshuam nrog tshuaj tua kab mob, 50% ~ 75% ntawm kev mob plab hnyuv uas cuam tshuam nrog tshuaj tua kab mob thiab 95% ~ 100% ntawm pseudomembranous enteritis yog tshwm sim los ntawm Clostridium difficile infection (CDI). Clostridium difficile yog ib qho kab mob uas muaj mob, suav nrog cov kab mob toxigenic thiab cov kab mob tsis-toxigenic.
Channel
| FAM | tcdAcov noob caj noob ces |
| ROX | tcdBcov noob caj noob ces |
| VIC/HEX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | lub rooj zaum |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Kev Tshwj Xeeb | siv cov khoom siv no los nrhiav lwm cov kab mob hauv plab xws li Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus thiab human genomic DNA, cov txiaj ntsig tsis zoo tag nrho. |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm SLAN-96P Cov Txheej Txheem PCR Real-Time (Hongshi Medical Technology Co., Ltd.) Lub Teeb Cicler®480 Lub Sijhawm PCR tiag tiag LineGene 9600 Plus Real-Time PCR Detection System(FQD-96A,HangzhouKev siv tshuab Bioer) MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag (Suzhou Molarray Co., Ltd.) BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Kev Xaiv 1.
Ntxiv 180μL ntawm lysozyme buffer rau hauv cov precipitate (dilute lysozyme rau 20mg / mL nrog lysozyme diluent), pipette kom sib tov zoo, thiab ua tiav ntawm 37 ° C rau ntau tshaj 30 feeb. Noj 1.5mL ntawm RNase / DNase-free centrifuge tube, thiab ntxiv180μL ntawm kev tswj hwm zoo thiab kev tswj hwm tsis zoo ua ke. Ntxiv10μL ntawm kev tswj sab hauv rau cov qauv uas yuav tsum tau kuaj, kev tswj zoo, thiab kev tswj tsis zoo ua ntu zus, thiab siv Nucleic Acid Extraction lossis Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd. rau kev rho tawm DNA qauv tom qab, thiab thov ua raws li cov lus qhia rau kev siv rau cov kauj ruam tshwj xeeb. Siv DNase/RNase dawb H2O rau elution, thiab qhov ntim elution pom zoo yog 100μL.
Kev Xaiv 2.
Siv 1.5mL ntawm lub raj centrifuge uas tsis muaj RNase/DNase, thiab ntxiv 200μL ntawm cov tshuaj tswj zoo thiab cov tshuaj tswj tsis zoo ua ke. Ntxiv10μL ntawm kev tswj sab hauv rau cov qauv uas yuav tsum tau kuaj, kev tswj zoo, thiab kev tswj tsis zoo ua ke, thiab siv Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv, thiab qhov ntim elution pom zoo yog 80μL.
