Clostridium difficile toxin A/B noob (C.diff)
Khoom npe
HWTS-OT031A Nucleic Acid Detection Kit rau Clostridium difficile toxin A/B noob (C.diff) (Fluorescence PCR)
Daim ntawv pov thawj
CE
Kab mob sib kis
Clostridium difficile (CD), gram-positive anaerobic sporogenic Clostridium difficile, yog ib qho ntawm cov kab mob tseem ceeb ua rau cov kab mob nosocomial plab hnyuv. Clinically, kwv yees li 15% ~ 25% ntawm cov tshuaj tiv thaiv kab mob raws plab, 50% ~ 75% ntawm cov tshuaj tiv thaiv kab mob sib kis thiab 95% ~ 100% ntawm pseudomembranous enteritis yog tshwm sim los ntawm Clostridium difficile infection (CDI). Clostridium difficile yog ib hom kab mob, nrog rau cov kab mob toxigenic thiab cov kab mob uas tsis yog toxigenic.
Channel
| FAM | tcdAnoob |
| ROX | tcdBnoob |
| VIC/HEX | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 12 hli |
| Hom Qauv | quav |
| Tt | ≤38 |
| CV | ≤ 5.0% |
| LoD | 200 CFU/ml |
| Qhov tshwj xeeb | siv cov khoom no los kuaj xyuas lwm cov kab mob hauv plab hnyuv xws li Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza human virus, DNA tshwm sim rau tib neeg. tag nrho cov tsis zoo. |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A,HangzhouBioer technology) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
Ntxiv 180μL ntawm lysozyme tsis nyob rau hauv precipitate (dilute lysozyme mus rau 20mg / mL nrog lysozyme diluent), pipette kom sib tov zoo, thiab txheej txheem ntawm 37 ° C rau ntau tshaj 30 feeb. Siv 1.5mL ntawm RNase / DNase-free centrifuge tube,180μL ntawm kev tswj zoo thiab kev tswj tsis zoo nyob rau hauv ib ntus. Ntxiv10μL ntawm kev tswj hwm sab hauv rau cov qauv yuav tsum tau kuaj, kev tswj zoo, thiab kev tswj tsis zoo hauv ntu, thiab siv Nucleic Acid Extraction lossis Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Siv DNase/RNase dawb H2O rau elution, thiab qhov pom zoo elution ntim yog 100μL.
Kev xaiv 2.
Noj 1.5mL ntawm RNase / DNase-dawb centrifuge raj, thiab ntxiv 200μL ntawm kev tswj zoo thiab kev tswj tsis zoo nyob rau hauv ib ntus. Ntxiv10μL ntawm kev tswj hwm sab hauv rau cov qauv yuav tsum tau kuaj, kev tswj zoo, thiab kev tswj tsis zoo hauv ntu, thiab siv Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid-0 (HWTS-3004-32). Kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv, thiab qhov ntim tau pom zoo yog 80μL.
