Clostridium didicile toxin A / B noob (C.diff)
Khoom npe
HWTS-OT031A Nucleic Acid kuaj tau rau Clostridium didicile toxin a / b noob noob (C.diff) (fluorescence pcr)
Daim ntawv muaj cai
CE
Kev kawm
Clostridium difficile (CD), A gram-zoo anaerobic sporogenic shorogenic shoricile classicile, yog ib qho ntawm cov kab mob tseem ceeb ua rau mob nosocomial. Clinically, kwv yees li 15% ~ 25% ntawm antimicrobial-ensimicrobial-omity antimitis-omeccess- 100% ntawm antimembous octitis yog tshwm sim los ntawm kev sib tw clostridium (CDI). Clostridium difficile yog kev ua kom muaj kev nyob zoo, suav nrog toxigenic strains thiab tsis-toxigenic strains.
Tshooj
| Tshaib plab | tcdaneeg |
| Nas | tcdbneeg |
| Vic / hex | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | quav quav |
| Tt | ≤38 |
| CV | APR5.0% |
| Lub lab | 200cfu / ml |
| Yam tshwj xeeb | Siv cov khoom siv no los kuaj xyuas lwm txoj kev rau lub plab me me, cov pa hluav taws, Rauv Thiaj li, tus kab mob salmana, tus mob khaub thuas, tus mob khaub thuas, tus mob khaub thuas barsenza b DNA, tus Cov txiaj ntsig tau yog txhua yam tsis zoo. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 REF-LUB SIJ HAWM PRRY Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler®480 REO-SIJ HAWM PRRICE Linegene 9600 Ntxiv rau cov sijhawm tshawb xyuas lub sijhawm tiag tiag (FQD-96A,HangzhouBioer Tshuab) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Qhov Xaiv 1.
Ntxiv 180μl ntawm lysozyme tsis mus rau cov precochyme rau 20mg / ml nrog lysozyme rau ntau dua 30 feeb.Thaum 1. DNSE-dawb Centrifuge raj, thiab ntxiv180μl ntawm kev tswj tau zoo thiab kev tswj hwm tsis zoo nyob rau hauv kab sib chaws. Ntxiv10μl ntawm kev tswj hwm sab hauv rau cov qauv uas yuav tsum tau kuaj, kev tswj hwm zoo nkauj, thiab siv cov bietchen nucen (YOMPENT) Co., Ltd. rau cov qauv DNA DNA extraction, thiab Thov ua raws li cov lus qhia siv rau cov kauj ruam tshwj xeeb. Siv DNase / rnase free h2O Rau kev lees paub, thiab cov kev pom zoo kom pom zoo yog 100μL.
Qhov Xaiv 2.
Nqa 1.5ml ntawm RNEase / dnase-dawb centrifuge raj, thiab ntxiv 200μl ntawm kev tswj zoo thiab kev tswj hwm tsis zoo nyob rau ntu. Ntxiv10μl ntawm kev tswj hwm sab hauv rau cov qauv uas yuav tsum tau kuaj, kev tswj hwm zoo, thiab siv cov tshuaj ntsuam 96) thiab macro & micro-xeem tsis siv neeg nucleic acid extract (HWTS-3006). Cov kev rho tawm yuav tsum tau nqa tawm hauv nruj ua raws li kev qhia rau kev siv, thiab cov lus pom zoo kom pom zoo tau pom zoo yog 80μL.
