Cov Khoom Siv Kuaj Xyuas Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus Nucleic Acid
Lub npe khoom
Cov Khoom Siv Kuaj Xyuas Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus Nucleic Acid (Fluorescence PCR)
Kev Siv Uas Xav Tau
Cov khoom siv no yog siv rau kev kuaj pom cov kab mob staphylococcus aureus thiab cov kab mob staphylococcus aureus uas tiv taus methicillin hauv cov qauv hnoos qeev tib neeg, cov qauv hnoos qeev hauv qhov ntswg thiab cov qauv kab mob ntawm daim tawv nqaij thiab cov nqaij mos hauv vitro. Cov qauv hnoos qeev thiab cov qauv hnoos qeev hauv qhov ntswg siv rau hauv cov tsev kho mob los tiv thaiv thiab tswj kev kis kab mob MRSA hauv cov neeg mob hauv tsev kho mob, suav nrog cov neeg mob hnyav, cov neeg mob phais thiab cov neeg mob mus sij hawm ntev; Nws kuj tseem siv tau rau kev kuaj mob pab rau cov neeg mob uas xav tias muaj kab mob Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus ua pa; Los yog ua ke nrog lwm yam kev kuaj hauv chaw kuaj mob xws li kev kuaj kab mob microbial los pab kuaj mob MRSA/SA ntawm daim tawv nqaij thiab cov nqaij mos. Cov txiaj ntsig ntawm kev kuaj mob tsis yog siv ncaj qha los qhia kev siv tshuaj tua kab mob.
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Staphylococcus aureus yog ib qho ntawm cov kab mob tseem ceeb ntawm kev kis tus kab mob hauv tsev kho mob. Staphylococcus aureus (SA) yog ib feem ntawm staphylococcus thiab yog tus sawv cev ntawm cov kab mob Gram-positive, uas tuaj yeem tsim ntau yam tshuaj lom thiab cov enzymes nkag mus rau hauv. Cov kab mob muaj cov yam ntxwv ntawm kev faib tawm dav dav, muaj zog pathogenicity thiab tus nqi tiv taus siab. Thermostable nuclease gene (nuc) yog ib qho gene khaws cia zoo ntawm staphylococcus aureus.
Nyob rau xyoo tas los no, vim muaj kev siv cov tshuaj hormones thiab cov tshuaj tiv thaiv kab mob ntau heev thiab kev siv cov tshuaj tua kab mob dav dav, kev kis kab mob hauv tsev kho mob uas tshwm sim los ntawm Methicillin-resistant Staphylococcus aureus (MRSA) hauv Staphylococcus tau nce siab. Lub teb chaws qhov nruab nrab ntawm kev kuaj pom MRSA yog 30.2% hauv xyoo 2019 hauv Suav teb. MRSA tau muab faib ua kev kho mob-cuam tshuam MRSA (HA-MRSA), zej zog-cuam tshuam MRSA (CA-MRSA), thiab tsiaj txhu-cuam tshuam MRSA (LA-MRSA). CA-MRSA, HA-MRSA, LA-MRSA muaj qhov sib txawv loj hauv microbiology, kev tiv thaiv kab mob (piv txwv li, HA-MRSA qhia tau tias muaj kev tiv thaiv ntau yam tshuaj dua li CA-MRSA) thiab cov yam ntxwv kho mob (piv txwv li qhov chaw kis kab mob). Raws li cov yam ntxwv no, CA-MRSA thiab HA-MRSA tuaj yeem paub qhov txawv. Txawm li cas los xij, qhov sib txawv ntawm CA-MRSA thiab HA-MRSA tab tom nqaim zuj zus vim muaj kev txav mus los tas li ntawm cov neeg ntawm tsev kho mob thiab cov zej zog. MRSA tiv taus ntau yam tshuaj, tsis yog tsuas yog tiv taus cov tshuaj tua kab mob β-lactam xwb, tab sis kuj tiv taus cov tshuaj aminoglycosides, macrolides, tetracyclines thiab quinolones rau ntau qib. Muaj qhov sib txawv loj heev hauv thaj chaw ntawm cov nqi tiv taus tshuaj thiab cov qauv sib txawv. MRSA tuaj yeem nkag mus rau hauv lub cev los ntawm cov qhov txhab ntawm daim tawv nqaij, cov plaub hau follicle, ntshav thiab lwm yam ua rau muaj kev kis kab mob, thiab cov neeg mob uas muaj kab mob ntawm daim tawv nqaij thiab kub hnyiab yog cov neeg mob uas muaj kev kis kab mob MRSA heev. Mob ntsws yog ib qho ntawm cov kab mob MRSA feem ntau, thiab kev kho mob tsis zoo yog qhov tseem ceeb ua rau muaj kev tuag ntau.
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤ -18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | Hnoos qeev, Qhov ntswg so, Daim tawv nqaij thiab cov nqaij mos mos kuaj kab mob, |
| CV | <5.0% |
| LoD | 1000 CFU/mL |
| Kev Tshwj Xeeb | Qhov kev sim tshuaj tiv thaiv kab mob qhia tau hais tias cov khoom siv no tsis muaj kev cuam tshuam nrog lwm cov kab mob ua pa xws li methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Cov Cuab Yeej Siv Tau | Hom I: Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Hom II: HWTS-Eudemon™ AIO800 |
Kev Ua Haujlwm
Cov tshuaj reagents thiab cov cuab yeej xav tau tab sis tsis tau muab
Hom I
(1) Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (Siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, 3006C).
(2) Cov qauv tshuaj diluent: 4% NaOH lossis Sputasol los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. (STXB 20220253)
(3) Cov kua ntsev ib txwm muaj.
Hom II:
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-8) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Cov khoom siv yuav tsum tau muab tab sis tsis tau muab:Cov Lus Qhia Tsis Muaj RNase/DNase, Cov hnab looj tes pov tseg, Cov raj EP tsis muaj RNase/DNase, 8-lub raj strips rau PCR.
