Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Lub npe khoom
HWTS-OT062 Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Daim Ntawv Pov Thawj
CE
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Staphylococcus aureus yog ib qho ntawm cov kab mob tseem ceeb ntawm kev kis tus kab mob hauv tsev kho mob. Staphylococcus aureus (SA) yog ib feem ntawm staphylococcus thiab yog tus sawv cev ntawm cov kab mob Gram-positive, uas tuaj yeem tsim ntau yam tshuaj lom thiab cov enzymes nkag mus rau hauv. Cov kab mob muaj cov yam ntxwv ntawm kev faib tawm dav dav, muaj zog pathogenicity thiab tus nqi tiv taus siab. Thermostable nuclease gene (nuc) yog ib qho gene khaws cia zoo ntawm staphylococcus aureus.
Channel
| FAM | cov noob caj noob ces mecA uas tiv taus methicillin |
| ROX | Kev Tswj Xyuas Sab Hauv |
| CY5 | staphylococcus aureus nuc gene |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ & tiv thaiv los ntawm lub teeb |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | cov qauv hnoos qeev, cov qauv kab mob ntawm daim tawv nqaij thiab cov nqaij mos, thiab cov qauv swab ntawm qhov ntswg |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL cov kab mob uas tiv taus methicillin. Thaum lub cuab yeej kuaj pom qhov kev siv LoD hauv tebchaws, 1000/mL staphylococcus aureus tuaj yeem kuaj pom |
| Kev Tshwj Xeeb | Qhov kev sim tshuaj tiv thaiv kab mob qhia tau hais tias cov khoom siv no tsis muaj kev cuam tshuam nrog lwm cov kab mob ua pa xws li methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm SLAN-96P Cov Txheej Txheem PCR Real-Time Lub Teeb Cicler®480 Lub Sijhawm PCR tiag tiag LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Kev Xaiv 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Ntxiv 200µL ntawm cov dej ntsev rau hauv cov dej uas tau ua tiav, thiab cov kauj ruam tom ntej yuav tsum tau rho tawm raws li cov lus qhia, thiab qhov ntim elution pom zoo yog 80µL.
Kev Xaiv 2.
Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ntxiv 1mL ntawm cov dej ntsev rau hauv cov dej nag tom qab ntxuav nrog cov dej ntsev, tom qab ntawd sib tov kom zoo. Centrifuge ntawm 13,000r/min rau 5 feeb, tshem tawm cov supernatant (khaws cia 10-20µL ntawm supernatant), thiab ua raws li cov lus qhia rau kev rho tawm tom qab.
Cov tshuaj reagent uas pom zoo kom rho tawm: Nucleic Acid Extraction or Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Kev rho tawm yuav tsum tau ua raws li kauj ruam 2 ntawm phau ntawv qhia. Nws raug pom zoo kom siv RNase thiab dej tsis muaj DNase rau kev elution nrog qhov ntim ntawm 100µL.
