Staphylococcus aureus thiab methicillin-resistant staphylococcus aureus (MRSA / SA)
Khoom npe
HWTS-ot062 StaphyRococcus aureus thiab Methicillin-resistant staphylococcus aureus (sa) nucleic acid nrhiav khoom siv (fluorescence pcr)
Daim ntawv muaj cai
CE
Kev kawm
Staphylococcus aureus yog ib qho ntawm cov kab mob tseem ceeb ntawm kev mob nosocomial. Staphylococcus aureus (sa) belongs rau cov kab mob staphylococcus thiab yog ib tus neeg sawv cev ntawm cov kab mob gram-zoo, uas tuaj yeem tsim ntau yam toxins thiab infzymes. Cov kab mob muaj cov yam ntxwv ntawm kev faib tawm dav, muaj zog pathogenicity thiab siab tiv taus tus nqi. Thermostable nuclase nuculase gene (NUC) yog ib tug gene conserved ntawm staphylococcus aureus.
Tshooj
| Tshaib plab | Methicillin-Resistant Meca Gene |
| Nas | Sab hauv tswj |
| Cy5 | Staphylococcus aureus nuc noob |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ & tiv thaiv txij lub teeb |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | Cov hnoos qeev, tawv nqaij thiab mos cov tshuaj kuaj kab mob, thiab Nasal Swab kuaj |
| Ct | I≤36 |
| CV | APR5.0% |
| Lub lab | 1000 CFU / ML Staphylococcus aureus, 1000 CFU / ML Methicillin-resistant cov kab mob. Thaum cov khoom siv kuaj pom cov National Lod hais txog, 1000 / ML Staphylococcus aureus tuaj yeem kuaj pom |
| Yam tshwj xeeb | Tus ntoo khaub lig-reactivity xeem qhia tau hais tias cov khoom siv no tsis muaj tus kab mob sib tsoo, pejtherase-rhiab staphyococcus epidermidis, coagerichia coli, eschuckas pneumoniae, acinetoBacter Baumannii, proteus mirabilis, Enterobacter Cneumoniae, Candionella Bneecium, Moralorella Catarrhalis, Moralosella Catarrhalis, MenomAria Meningitidis, NomoPhilia meningdidias, Haemophilus influkae. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab QuatStudio®5 REF-LUB SIJ HAWM PRRY Slan-96p ROOST-SICTICE PRRY Lightcycler®480 REO-SIJ HAWM PRRICE LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEV Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Qhov Xaiv 1.
Macro & micro-kev ntsuas Genomic DNA / RNA cov khoom siv (HWTS-3019) los ntawm Jiangsu Macro & Micro-Test Acid Extraction (HWTS-3006C, HWTS- 3006b). Ntxiv 200μL ntawm cov saline ib txwm rau cov txheej txheem ua ntej, thiab tom qab cov kauj ruam tom ntej yuav tsum tau muab rho tawm raws li cov lus qhia, thiab cov lus qhia pom zoo kom pom zoo yog 80μL.
Qhov Xaiv 2.
Macro & micro-xeem piv tawm reagent (hwts-3005-8) los ntawm ib txwm saline rau cov nag lossis ntxuav cov dej qab zib, ces sib tov zoo. Centrifuge thaum 13,000r / min rau 5 feeb, tshem tawm cov supernatant (Reserve 10-204L ntawm supernatant), thiab ua raws cov lus qhia rau kev tshem tawm tom qab extraction.
Pom zoo rho tawm reagent: nucleic acid extraction lossis purification reagent (ydp32) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Kev rho tawm yuav tsum tau nqa tawm nruj me ntsis raws li theem 2 ntawm phau ntawv qhia. Nws raug nquahu kom siv RNEase thiab DNase-dej dawb rau kev xaiv nrog lub ntim ntawm 100μl.
