Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Khoom npe
HWTS-OT062 Staphylococcus Aureus thiab Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE
Kab mob sib kis
Staphylococcus aureus yog ib qho ntawm cov kab mob tseem ceeb ntawm cov kab mob nosocomial.Staphylococcus aureus (SA) belongs rau staphylococcus thiab yog tus sawv cev ntawm cov kab mob Gram-zoo, uas tuaj yeem tsim ntau yam co toxins thiab invasive enzymes.Cov kab mob muaj cov yam ntxwv ntawm kev sib faib dav, muaj zog pathogenicity thiab siab tsis kam.Thermostable nuclease gene (nuc) yog ib hom kev txuag ntawm staphylococcus aureus.
Channel
| FAM | methicillin-resistant mecA noob |
| ROX | Internal Control |
| CY 5 | Staphylococcus aureus nuc noob |
Technical Parameters
| Cia | ≤-18 ℃ & tiv thaiv los ntawm lub teeb |
| Txee lub neej | 12 hli |
| Hom Qauv | cov hnoos qeev, daim tawv nqaij thiab cov ntaub so ntswg mos kab mob, thiab kuaj qhov ntswg qhov ntswg |
| Ct | ≤36 |
| CV | ≤ 5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL methicillin-resistant kab mob.Thaum cov khoom siv kuaj pom lub teb chaws LoD siv, 1000 / mL staphylococcus aureus tuaj yeem kuaj pom |
| Qhov tshwj xeeb | Qhov kev kuaj hla-reactivity qhia tau hais tias cov khoom siv no tsis muaj qhov cuam tshuam nrog lwm cov kab mob ua pa xws li methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aerocococcus aeruginosa, coagulase-negative staphylococcus. baumannii, cov Mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Ntxiv 200µL ntawm ib txwm saline rau cov txheej txheem precipitate, thiab cov kauj ruam tom ntej yuav tsum tau muab rho tawm raws li cov lus qhia, thiab qhov pom zoo elution ntim yog 80µL.
Kev xaiv 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ntxiv 1mL ntawm ib txwm saline rau lub precipitate tom qab ntxuav nrog ib txwm saline, ces sib tov zoo.Centrifuge ntawm 13,000r / min rau 5 feeb, tshem tawm cov supernatant (tso 10-20µL ntawm supernatant), thiab ua raws li cov lus qhia rau kev rho tawm tom ntej.
Pom zoo extraction reagent: Nucleic Acid Extraction lossis Purification Reagent (YDP302) los ntawm Tiangen Biotech (Beijing) Co., Ltd.Kev rho tawm yuav tsum tau ua nruj me ntsis raws li cov kauj ruam 2 ntawm phau ntawv qhia.Nws raug nquahu kom siv RNase thiab DNase-dawb dej rau elution nrog ntim ntawm 100µL.
