Zaire Ebola tus kab mob
Khoom npe
HWTS-FE008 Zaire Ebola Virus Nucleic Acid kuaj tau khoom (fluorescence pcr)
Kev kawm
Tus kab mob Ebola belongs rau Filoviridae, uas yog ib qho tsis sib haum xeeb-stranded tsis zoo-Strand RNA tus kab mob. Cov kab mob yog cov filaments ntev nrog tus virity nruab nrab ntev ntawm 1000nm thiab ib lub cheeb ntawm txog 100nm. Tus kab mob Ebola yog qhov tsis txaus ntseeg yog ib qho tsis zoo RNA nrog qhov loj ntawm 18.9kb, encoding 7 cov protein thiab 1 tsis-qauv protein. Tus kab mob Ebola tuaj yeem faib ua hom zoo li Zaire, Sudan, Bundibugyo, Tai hav zoov thiab rov qab. Ntawm lawv, Zaire Hom thiab Sudan hom tau tshaj tawm tias yuav ua rau ntau tus neeg tuag los ntawm kev kis mob. EHF (Ebola hemorrhagic npaws) yog ib tus kab mob hemorrhagic tau sib kis los ntawm tus kab mob Ebola. Tib neeg tsuas yog kis los ntawm kev sib chwv lub cev, zais cia thiab cov tsiaj kho mob yog feem ntau tiv thaiv ua npaws, ntshav thiab ntau yam kev puas tsuaj hauv nruab nrog cev. EHF muaj lub siab tuag ntawm 50% -90%.
Tshooj
| Tshaib plab | Mp nucleic acid |
| Nas | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ |
| Txee-Lub Neej | 9 lub hlis |
| Zauv Hom | Fresh ntshiab, ntshav |
| Tt | ≤38 |
| CV | APR5.0% |
| Lub lab | 500 daim / μl |
| Yam tshwj xeeb | Siv cov khoom siv los kuaj tuam txhab cov tuam txhab tsis zoo, cov txiaj ntsig tau ua tiav kom ua. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p RO-LUB SIJHAWM PRR TSHUAB (Hongshi Medical Technology Co., Ltd.) Lightcycler ®480 REO-SIJHAWM PCR system Linegene 9600 Ntxiv rau Kev Ntsuas Phaj Phaj Real-Time (FQD-96A, Hangzhou Biorer Technology) Ma-6000 tiag tiag-lub sij hawm tiag tiag cov cua sov thizler (Suzhou Molarray Co., Ltd.) Biorad Cfx96 REF-SO-SIPR system, thiab Biorad Cfx Open 96 Real-Time PcR system |
Ua Haujlwm Ntws
Qhov Xaiv 1.
Pom zoo rho tawm reagent: Qiaamp viral rana cov khoom siv (52904), nucleic reid extraction lossis purification reident (ydp315-r) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Nws yuav tsum tau muab rho tawm nyob rau hauv nruj ua raws li cov lus qhia, thiab cov kev pom zoo muab kev pom zoo tau ntim ntawm cov qauv yog 140μL thiab cov lus pom zoo kom pom zoo yog 60μL.
Qhov Xaiv 2.
Pom zoo rho tawm reagent: Macro & RNO KIT (HWTS-3004-32, yuav tsum muab rho tawm raws li cov lus qhia. Cov kev rho tawm cov qauv ntim yog 200μL, thiab cov lus pom zoo kom meej meej ntim yog 80μL.
Qhov Xaiv 3.
Pom zoo rho tawm cov khoom seem: nucleic acid extraction reagent (10000201) thiab siab dhau mus rau qhov qauv uas yuav tsum tau muab rho tawm raws li cov lus qhia. Cov kev rho tawm yog 160μl, thiab cov lus pom zoo kom pom zoo yog 60μl.
