Zaire Ebola Virus
Khoom npe
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Tus kab mob Ebola belongs rau Filoviridae, uas yog ib qho tsis zoo ib leeg-stranded tsis zoo-strand RNA tus kab mob.Cov kab mob yog cov kab mob ntev nrog qhov nruab nrab virion ntev ntawm 1000nm thiab txoj kab uas hla ntawm 100nm.Tus kab mob Ebola genome yog unsegmented tsis zoo-strand RNA nrog ib tug loj ntawm 18.9kb, encoding 7 structural proteins thiab 1 non-structural protein.Tus kab mob Ebola tuaj yeem muab faib ua hom xws li Zaire, Sudan, Bundibugyo, Tai Forest thiab Reston.Ntawm lawv, hom Zaire thiab Sudan hom tau tshaj tawm tias ua rau ntau tus neeg tuag los ntawm kev kis kab mob.EHF (Ebola Hemorrhagic Fever) yog ib hom kab mob hemorrhagic kis los ntawm tus kab mob Ebola.Tib neeg feem ntau kis tau los ntawm kev sib cuag nrog cov kua hauv lub cev, tso tawm thiab tso tawm ntawm cov neeg mob lossis cov tsiaj muaj kab mob, thiab cov tsos mob tshwm sim feem ntau yog ua npaws, los ntshav thiab ntau yam kabmob.EHF muaj kev tuag siab ntawm 50% -90%.
Channel
| FAM | MP nucleic acid |
| ROX | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | Fresh serum, Plasma |
| Tt | ≤38 |
| CV | ≤ 5.0% |
| LoD | 500 Daim / μL |
| Qhov tshwj xeeb | Siv cov khoom siv los ntsuas lub tuam txhab cov ntaub ntawv tsis zoo, cov txiaj ntsig tau raws li qhov xav tau. |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer thev naus laus zis) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, thiab BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
Pom zoo extraction reagent: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction lossis Purification Reagent (YDP315-R) los ntawm Tiangen Biotech (Beijing) Co., Ltd.Nws yuav tsum tau muab rho tawm kom nruj raws li cov lus qhia, thiab cov lus pom zoo muab rho tawm ntawm cov qauv yog 140μL thiab qhov pom zoo elution ntim yog 60μL.
Kev xaiv 2.
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). yuav tsum tau muab rho tawm raws li cov lus qhia.Cov qauv rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 80μL.
Kev xaiv 3.
Pom zoo extraction reagents: Nucleic Acid Extraction Reagent (1000020261) thiab High Throughput Automated Sample Preparation System (MGISP-960) los ntawm BGI yuav tsum tau muab rho tawm raws li cov lus qhia.Lub ntim ntim yog 160μL, thiab qhov pom zoo elution ntim yog 60μL.
