Kab mob Ebola hauv Zaire
Lub npe khoom
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Tus kab mob Ebola yog ib hom kab mob uas muaj ib txoj kab RNA uas tsis muaj kab sib txuas. Cov kab mob no yog cov filaments ntev nrog qhov ntev ntawm virion nruab nrab ntawm 1000nm thiab txoj kab uas hla ntawm kwv yees li 100nm. Lub genome ntawm tus kab mob Ebola yog ib txoj kab RNA uas tsis muaj kab sib txuas nrog qhov loj ntawm 18.9kb, encoding 7 cov protein structural thiab 1 cov protein uas tsis muaj kab sib txuas. Tus kab mob Ebola muaj peev xwm muab faib ua hom xws li Zaire, Sudan, Bundibugyo, Tai Forest thiab Reston. Ntawm lawv, hom Zaire thiab hom Sudan tau tshaj tawm tias ua rau ntau tus neeg tuag los ntawm kev kis tus kab mob. EHF (Ebola Hemorrhagic Fever) yog ib hom kab mob sib kis los ntawm tus kab mob Ebola. Tib neeg feem ntau kis tau los ntawm kev sib cuag nrog cov kua dej hauv lub cev, cov kua qaub thiab cov quav ntawm cov neeg mob lossis cov tsiaj uas kis tus kab mob, thiab cov tsos mob ntawm tus kab mob feem ntau yog kub taub hau, los ntshav thiab ntau yam kab mob puas tsuaj. EHF muaj tus nqi tuag siab ntawm 50% -90%.
Channel
| FAM | MP nucleic acid |
| ROX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | Cov serum tshiab, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Daim Ntawv Luam / μL |
| Kev Tshwj Xeeb | Siv cov khoom siv los sim cov ntaub ntawv tsis zoo ntawm lub tuam txhab, cov txiaj ntsig ua tau raws li qhov yuav tsum tau ua. |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev Cov Txheej Txheem PCR QuantStudio®5 Real-Time SLAN-96P Cov Txheej Txheem PCR Real-Time (Hongshi Medical Technology Co., Ltd.) Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology) MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, thiab BioRad CFX Opus 96 Real-Time PCR System |
Kev Ua Haujlwm
Kev Xaiv 1.
Cov tshuaj reagent uas pom zoo kom rho tawm: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) los ntawm Tiangen Biotech (Beijing) Co.,Ltd. Nws yuav tsum tau rho tawm raws li cov lus qhia, thiab qhov ntim ntawm cov qauv rho tawm uas pom zoo yog 140μL thiab qhov ntim ntawm cov kua dej uas pom zoo yog 60μL.
Kev Xaiv 2.
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Yuav tsum tau rho tawm raws li cov lus qhia. Qhov ntim ntawm cov qauv rho tawm yog 200μL, thiab qhov ntim ntawm cov kua dej uas pom zoo kom rho tawm yog 80μL.
Kev Xaiv 3.
Cov tshuaj reagents uas pom zoo kom rho tawm: Nucleic Acid Extraction Reagent (1000020261) thiab High Throughput Automated Sample Preparation System (MGISP-960) los ntawm BGI yuav tsum tau rho tawm raws li cov lus qhia. Qhov ntim rho tawm yog 160μL, thiab qhov ntim elution uas pom zoo kom rho tawm yog 60μL.
