15 Hom Kab Mob Papillomavirus E6/E7 Gene mRNA Uas Muaj Kev Pheej Hmoo Heev
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HWTS-CC005A-15 Hom Kab Mob Papillomavirus E6/E7 Gene mRNA Detection Kit Uas Muaj Kev Pheej Hmoo Heev (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob qog nqaij hlav hauv lub tsev menyuam yog ib hom kab mob qog nqaij hlav uas pom ntau tshaj plaws hauv ntiaj teb, thiab nws qhov tshwm sim muaj feem cuam tshuam nrog cov kab mob papillomavirus (HPV), tab sis tsuas yog ib feem me me ntawm cov kab mob HPV thiaj li loj hlob mus ua mob qog nqaij hlav. HPV uas muaj kev pheej hmoo siab kis mus rau cov hlwb epithelial ntawm lub tsev menyuam thiab tsim ob hom oncoproteins, E6 thiab E7. Cov protein no tuaj yeem cuam tshuam rau ntau yam protein ntawm lub hlwb (xws li cov protein tiv thaiv qog nqaij hlav pRB thiab p53), ua kom lub voj voog ntawm lub hlwb ntev dua, cuam tshuam rau DNA synthesis thiab genome stability, thiab cuam tshuam rau cov lus teb tiv thaiv kab mob thiab tiv thaiv qog nqaij hlav.
Channel
| Channel | Cheebtsam | Tau kuaj genotype |
| FAM | HPV Cov Tshuaj Tiv Thaiv 1 | HPV 16, 31, 33, 35, 51, 52, 58 |
| VIC/HEX | Tib neeg β-actin gene | |
| FAM | HPV Reaction Buffer 2 | HPV 18, 39, 45, 53, 56, 59, 66, 68 |
| VIC/HEX | Cov noob caj noob ces INS ntawm tib neeg |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | Kua: ≤-18 ℃ |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | Daim ntaub so caj dab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Daim Ntawv Luam / mL |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Lub Tshuab PCR Tiag-Lub SijhawmApplied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev Cov Txheej Txheem PCR QuantStudio®5 Real-Time SLAN-96P Cov Txheej Txheem PCR Real-Time Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv. Lub ntim elution pom zoo yog 50μL. Yog tias cov qauv tsis tau zom tag, rov qab mus rau kauj ruam 4 rau kev zom dua. Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.
Cov tshuaj reagent uas pom zoo kom rho tawm: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Kev rho tawm yuav tsum ua raws li cov lus qhia rau kev siv nruj me ntsis (Hauv kauj ruam 5, ob npaug ntawm qhov concentration ntawm DNaseI ua haujlwm kua, uas yog, coj 20μL ntawm RNase-Free DNaseI (1500U) kua Tshuag rau hauv lub raj centrifuge RNase-Free tshiab, ntxiv 60μL ntawm RDD buffer, thiab sib tov maj mam). Qhov ntim elution pom zoo yog 60μL. Yog tias cov qauv tsis tau zom tag nrho, rov qab mus rau kauj ruam 5 rau kev zom dua. Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.
