15 hom kev pheej hmoo siab ntawm tib neeg papillomavirus E6 / E7 Gene MRN
Khoom npe
HWTS-CC005A-15 hom kev pheej hmoo high-pheej yig tib neeg papillomavirus E6 / E7 Gene MRA cov khoom siv (fluorescence pcr)
Kev kawm
Kab mob cancer yog ib hom Cancere Cancer Feem Ntau Muaj feem cuam tshuam nrog tib neeg Papillomaviruses (HPV), tab sis tsuas yog ib qho kev kis mob HPV tuaj yeem muaj mob cancer. Kev pheej hmoo HPV kis tau mob siab rau cervical epithelial hlwb thiab tsim ob lub oncoproteins, E6 thiab E7. Qhov protein no tuaj yeem cuam tshuam rau ntau yam tshuaj pleev cov qog noj mov (xws li lub voj voog suppression, cuam tshuam nrog cov tshuaj tua kab mob thiab kev tiv thaiv kab mob tawm tsam.
Tshooj
| Tshooj | Ib feem | Genotype Tested |
| Tshaib plab | HPV cov tshuaj tiv thaiv tsis 1 | HPV16,31,33,35,51,52,58 |
| Vic / hex | Neeg β-actin gene | |
| Tshaib plab | HPV cov tshuaj tiv thaiv tsis 2 | HPV 18,39,53,56,59,66,68 |
| Vic / hex | Tib neeg ins gene |
Kev tsis muaj
| Chaw ntim khoom | Cov kua: ≤-18 ℃ |
| Txee-Lub Neej | 9 lub hlis |
| Zauv Hom | Cervical Swab |
| Ct | ≤38 |
| CV | <0% |
| Lub lab | 500 daim / ml |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRRICESiv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p ROOST-SICTICE PRRY Lightcycler ®480 REO-SIJHAWM PCR system LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEV Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Pom zoo rho tawm reagent: Macro & Cov Khoom Siv Hlau Cov. Cov lus pom zoo pom zoo yog 50μL. Yog tias cov qauv tsis yog tas zom, rov qab rau kauj ruam 4 rau kev rov ua dua. Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.
Pom zoo rho tawm reagent: rnaprep ntshiab cov tsiaj ntshiab nqaij Tag nrho RNA RNA cov khoom siv extraction (DP431). Cov kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv nruj me ntsis (ntawm cov kev daws teeb meem, uas yog siv sijhawm ua haujlwm ntawm Rnesase Rnesase Rnesha-dawb Centrifuge Tube, Ntxiv 60μL ntawm RDD tsis, thiab sib xyaw maj mam). Cov tshuaj pom zoo kom pom zoo yog 60μL. Yog tias cov qauv tsis tiav kiag li, xa rov qab rau kauj ruam 5 rau kev rov ua dua tshiab. Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.
