15 Hom Kev pheej hmoo siab Human Papillomavirus E6/E7 Gene mRNA

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Cov khoom siv no yog tsom rau qhov kev kuaj pom zoo ntawm 15 tus neeg muaj kev pheej hmoo siab human papillomavirus (HPV) E6/E7 gene mRNA qhia qib hauv cov hlwb exfoliated ntawm poj niam lub ncauj tsev menyuam.


Product Detail

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HWTS-CC005A-15 Hom kev pheej hmoo siab Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)

Kab mob sib kis

Kev mob khees xaws ncauj tsev menyuam yog ib yam ntawm cov poj niam mob qog noj ntshav thoob plaws ntiaj teb, thiab nws qhov tshwm sim muaj feem xyuam nrog tib neeg papillomaviruses (HPV), tab sis tsuas yog ib feem me me ntawm cov kab mob HPV tuaj yeem ua rau mob qog noj ntshav.High-risk HPV kis kab mob ntawm ncauj tsev menyuam epithelial hlwb thiab tsim ob oncoproteins, E6 thiab E7.Cov protein no tuaj yeem cuam tshuam rau ntau yam cellular proteins (xws li qog nqaij hlav proteins pRB thiab p53), ua kom lub voj voog ntawm tes, cuam tshuam rau DNA synthesis thiab genome stability, thiab cuam tshuam nrog cov tshuaj tiv thaiv kab mob thiab tshuaj tiv thaiv kab mob.

Channel

Channel Cheebtsam Genotype kuaj
FAM HPV Reaction Buffer 1 HPV 16, 31, 33, 35, 51, 52, 58
VIC/HEX Tib neeg beta-actin gene
FAM HPV Reaction Buffer 2 HPV 18, 39, 45, 53, 56, 59, 66, 68
VIC/HEX Tib neeg INS gene

Technical Parameters

Cia Ua kua: ≤-18 ℃
Txee lub neej 9 muaj
Hom Qauv Cervical swab
Ct ≤38
CV <5.0%
LoD 500 daim / ml
Cov cuab yeej siv tau Siv Biosystems 7500 Real-Time PCR SystemSiv Biosystems 7500 Fast Real-Time PCR Systems

QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

LightCycler® 480 Real-Time PCR system

LineGene 9600 Plus Real-Time PCR Detection System

MA-6000 Real-Time Quantitative Thermal Cycler

BioRad CFX96 Real-Time PCR System

BioRad CFX Opus 96 Real-Time PCR System

Ua hauj lwm Flow

Pom zoo extraction reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Cov extraction yuav tsum tau ua nruj me ntsis raws li cov lus qhia siv .Qhov pom zoo elution ntim yog 50μL.Yog tias tus qauv tsis zom tag nrho, rov qab mus rau qib 4 rau kev rov ua dua tshiab.Thiab tom qab ntawd sim raws li cov lus qhia rau kev siv.

Pom zoo extraction reagent: RNAprep Ntshiab Tsiaj Tissue Total RNA Extraction Kit (DP431).Kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv nruj me ntsis (Nyob rau hauv kauj ruam 5, muab ob npaug rau cov concentration ntawm DNaseI ua hauj lwm tov, uas yog, noj 20μL ntawm RNase-Free DNaseI (1500U) Tshuag tov rau hauv ib tug tshiab RNase-Free centrifuge raj, ntxiv 60μL ntawm RDD tsis, thiab sib tov maj mam).Qhov pom zoo elution ntim yog 60μL.Yog tias tus qauv tsis tau zom tag nrho, rov qab mus rau qib 5 rau kev rov ua dua tshiab.Thiab ces sim raws li cov lus qhia rau kev siv.


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