17 hom HPV (16/18/6/11/44 Ntaus)
Khoom npe
HWTS-CC015 17 hom ntawm tib neeg Papillomaviras (16/18/6/11/44 ntaus) nucleic acid nrhiav tau khoom (fluorescence pcr)
Kev kawm
Cleervical Cancer yog ib qho ntawm feem ntau cov kev ntxub ntxaug hauv cov poj niam ua me nyuam. Nws tau pom tias pheej muaj mob HPV thiab ntau yam kev mob yog ib qho kev ua rau menyuam kab mob cervical. Tam sim no tseem muaj qhov tsis muaj kev tsis lees txais kev kho mob kom zoo rau cov menyuam kab mob cancer los ntawm HPV. Yog li ntawd, kev soj ntsuam ntxov thiab tiv thaiv kab mob ncauj tsev menyuam los ntawm HPV yog cov yuam sij los tiv thaiv mob cleervical. Tsim cov kev sim yooj yim, kev kuaj mob sai sai thiab kev kuaj mob sai sai rau cov pathogens yog qhov tseem ceeb ntawm kev kuaj mob hauv lub nroog cancer.
Tshooj
| PCR-Mix1 | Tshaib plab | 18 |
| Vic / hex | 16 | |
| Nas | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| Cy5 | Sab hauv tswj |
| PCR-Mix2 | Tshaib plab | 6 |
| Vic / hex | 11 | |
| Nas | 44 | |
| Cy5 | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | -18 ℃ |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | Cov zis ua piv txwv, poj niam cov ncauj tsev menyuam pob zeb, poj niam ntawm kev sib tw |
| Ct | PCT28 |
| Lub lab | 300copies / ml |
| Yam tshwj xeeb | Tsis muaj tus ntoo khaub lig-reactivity nrog urroplasma ureazyticum, chlamydia ntawm kev ua me nyuam, pwm, gardnerella thiab lwm hom HPV tsis duav los ntawm cov khoom siv. |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 REF-LUB SIJ HAWM PRRY Slan-96p ROOST-SICTICE PRRY Lightcycler®480 REO-LUB SIJ HAWM PRRY LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM TAWM TSHUAB Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Pom zoo rho tawm reagent: Tsis siv neeg nucleic acid extraction (hwts-3006c, hwts-3006b)) los ntawm Jiangsu Macro & Kev ntsuas me me me me. Cov tshuaj pom zoo kom pom zoo yog 80μL.
Pom zoo rho tawm reagent: Qiaamp DNA Mini Khoom (51304) lossis Macro & micro-Kev Ntsuas Kab Mob (HWTS-3020-50). Ntxiv 200μL ntawm cov saline ib txwm mus rau resuspend pellet nyob rau hauv kauj ruam 2.1, thiab tom qab ntawd extraction yuav tsum tau ua raws li cov lus qhia rau kev siv cov tshuaj no. Cov kev rho tawm cov qauv ntim ntawm cov qauv yog txhua xyoo 200μL, thiab cov kev pom zoo kom pom zoo yog 100μL.
Pom zoo rho tawm reagent: macro & micro-ntsuas piv txwv tso tawm (HWTS-300. Ntxiv 200μL ntawm cov piv txwv tso tawm REGENT los resuspend lub pellet nyob rau hauv kauj ruam 2.1, thiab tom qab ntawd extraction yuav tsum tau ua raws li cov lus qhia rau kev siv cov tshuaj no.
