28 hom kev pheej hmoo ntawm tib neeg tus kab mob papillomal (16/18 ntaus) nucleic acid
Khoom npe
HWTS-CC006A-28 hom kev pheej hmoo siab ntawm Tib Neeg Papilloma Virus (16/18 ntaus) nucleic acid nrhiav tau khoom (fluorescence pcr)
Kev kawm
Creatical Cancer yog ib qho ntawm feem ntau cov kev ntxub ntxaug ntawm cov poj niam ua me nyuam. Cov kev tshawb fawb tau pom tias HPV cov kab mob tsis tu ncua thiab ntau yam kev mob yog ib qho kev ua rau menyuam kab mob cortus. Tam sim no, txoj kev kho tau zoo yog tseem tsis tau txais cov kab mob pob menyuam yaus tshwm sim los ntawm HPV, yog li kev tiv thaiv thaum ntxov los tiv thaiv mob ncauj tsev menyuam. Nws yog qhov tseem ceeb tshaj plaws los tsim kom muaj qhov yooj yim, kev kuaj mob thiab kev kuaj mob sai sai thiab kev kho mob cervical.
Tshooj
| Cov tshuaj tiv thaiv sib tov | Tshooj | Ntaus |
| PCR-Mix1 | Tshaib plab | 18 |
| Vic (Hex) | 16 | |
| Nas | 31, 33, 35, 45, 45, 51, 52, 56, 58, 59, 66, 66, 66, 68 | |
| Cy5 | Sab hauv tswj | |
| PCR-Mix2 | Tshaib plab | 6, 11, 54, 83 |
| Vic (Hex) | 26, 44, 61, 81 | |
| Nas | 40, 42, 43, 53, 73, 82 | |
| Cy5 | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | Cov kua: ≤-18 ℃ |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | Lub tsev kawm ntawv sib tsoo, lub paum ntaj ntsug, tso zis |
| Ct | PCT28 |
| CV | <0% |
| Lub lab | 300copies / ml |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 REF-LUB SIJ HAWM PRRY Slan-96p ROOST-SICTICE PRRY Lightcycler®480 REO-SIJ HAWM PRRICE LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEV Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Pom zoo rho tawm reagent: Tsis siv neeg nucleic acid extraction (hwts-3006c, hwts-3006b)) los ntawm Jiangsu Macro & Kev ntsuas me me me me. Cov tshuaj pom zoo kom pom zoo yog 80μL.
Pom zoo rho tawm reagent: Qiaamp DNA Mini Khoom (51304) lossis Macro & micro-Kev Ntsuas Kab Mob (HWTS-3020-50). Ntxiv 200μL ntawm cov saline ib txwm mus rau resuspend pellet nyob rau hauv kauj ruam 2.1, thiab tom qab ntawd extraction yuav tsum tau ua raws li cov lus qhia rau kev siv cov tshuaj no. Cov kev rho tawm cov qauv ntim ntawm cov qauv yog txhua xyoo 200μL, thiab cov kev pom zoo kom pom zoo yog 100μL.
