Coxsackie Virus Type A16 Nucleic Acid

Cov khoom siv no siv Enzymatic Probe Isothermal Amplification (EPIA), thiab tsim cov primers tshwj xeeb thiab RNA puag-muaj kev sojntsuam (rProbe) rau thaj tsam uas tau txais txiaj ntsig zoo ntawm Cox A16, thiab ntxiv Bst enzyme thiab RNaseH enzyme tib lub sijhawm, nyob rau hauv uas sab laug thiab txoj cai kawg ntawm RNA puag ntawm rProbe yog sau nrog fluorescent pawg thiab quencher, raws li.Siv cov DNA polymerase kev ua si thiab strand hloov chaw ntawm Bst enzyme los ua kom lub hom phiaj kom raug kuaj ntawm qhov kub thiab txias, RNaseH enzyme tuaj yeem tshem tawm RNA hauv paus ntawm lub hom phiaj-kev sojntsuam hybrid saw, kom cov fluorescent pawg thiab quencher ntawm rProbe yog cais li no fluorescing.Tsis tas li ntawd, cov seem seem ntawm cov seem rProbe RNA puag tuaj yeem siv los ua primer kom txuas ntxiv mus rau kev tsim cov khoom, uas ntxiv cov khoom ntxiv.Lub teeb liab fluorescent txuas ntxiv mus nrog kev tsim cov khoom, yog li paub txog kev tshawb pom ntawm lub hom phiaj nucleic acid.