Kab mob Enterovirus 71 (EV71)
Lub npe khoom
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob tes-taw-qhov ncauj yog ib yam kab mob sib kis los ntawm cov kab mob enteroviruses (EV). Tam sim no, muaj 108 hom kab mob enteroviruses, uas muab faib ua plaub pawg: A, B, C thiab D. Ntawm lawv, enterovirus EV71 thiab CoxA16 yog cov kab mob tseem ceeb. Cov kab mob no feem ntau tshwm sim rau cov menyuam yaus hnub nyoog qis dua 5 xyoos, thiab tuaj yeem ua rau mob herpes rau ntawm tes, taw, qhov ncauj thiab lwm qhov chaw. Muaj ob peb tug menyuam yaus uas yuav muaj teeb meem xws li mob myocarditis, mob ntsws o, thiab mob meningoencephalitis.
Channel
| FAM | EV71 |
| ROX | Kev Tswj Xyuas Sab Hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | Oropharyngeal swab,Cov kua dej Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Daim/mL |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev QuantStudio®5 Lub Tshuab PCR Tiag-Lub Sijhawm SLAN-96P Cov Txheej Txheem PCR Real-Time Lub Teeb Cicler®480 Lub Tshuab PCR Tiag-Lub Sijhawm LineGene 9600 Plus Cov Tshuab Tshawb Nrhiav PCR Lub Sijhawm Tiag Tiag MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Kev Xaiv 1.
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), thiab kev rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv. Cov qauv rho tawm yog cov swabs oropharyngeal lossis cov kua dej herpes los ntawm cov neeg mob uas tau sau rau ntawm qhov chaw. Ntxiv cov swabs uas tau sau rau hauv Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ncaj qha, vortex thiab sib tov zoo, muab tso rau hauv chav sov li 5 feeb, coj lawv tawm thiab tom qab ntawd tig rov qab kom sib tov zoo, kom tau txais RNA ntawm txhua tus qauv.
Kev Xaiv 2.
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., thiab kev rho tawm yuav tsum ua raws li cov lus qhia rau kev siv. Qhov ntim ntawm cov qauv rho tawm yog 200µL, thiab qhov ntim ntawm cov kua dej uas pom zoo kom rho tawm yog 80µL.
Kev Xaiv 3.
Cov tshuaj reagent uas pom zoo kom rho tawm: QIAamp Viral RNA Mini Kit (52904) los ntawm QIAGEN lossis TIANamp Virus DNA/RNA Kit (YDP315-R), thiab kev rho tawm yuav tsum ua raws li cov lus qhia siv. Qhov ntim ntawm cov qauv rho tawm yog 140μL, thiab qhov ntim ntawm cov kua dej uas pom zoo kom rho tawm yog 60µL.
