Enterovirus 71 (EV71)
Khoom npe
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Cov kab mob ntawm tes-ko taw-qhov ncauj yog kab mob kis los ntawm enteroviruses (EV).Tam sim no, 108 hom kab mob enteroviruses tau pom, uas tau muab faib ua plaub pawg: A, B, C thiab D. Ntawm lawv, enterovirus EV71 thiab CoxA16 yog cov kab mob tseem ceeb.Tus kab mob no feem ntau tshwm sim rau cov menyuam yaus hnub nyoog qis dua 5 xyoos, thiab tuaj yeem ua rau mob herpes ntawm tes, taw, qhov ncauj thiab lwm qhov chaw.Qee tus menyuam yaus yuav muaj teeb meem xws li myocarditis, pulmonary edema, thiab aseptic meningoencephalitis.
Channel
| FAM | EV 71 |
| ROX | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | Oropharyngeal swb,Herpes kua dej |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 daim / ml |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Kev xaiv 1.
Pom zoo extraction reagent: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), thiab cov extraction yuav tsum tau ua raws li cov lus qhia rau kev siv.Cov qauv muab rho tawm yog cov tshuaj oropharyngeal swabs lossis herpes kua kuaj los ntawm cov neeg mob uas tau sau rau ntawm qhov chaw.Ntxiv cov swabs sau rau hauv Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) ncaj qha, vortex thiab sib tov zoo, tso rau hauv chav sov li 5 feeb, coj lawv tawm thiab tom qab ntawd hloov mus rau qhov sib tov zoo, kom tau txais RNA ntawm txhua tus qauv.
Kev xaiv 2.
Pom zoo extraction reagent: Macro & Micro-Test General DNA / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Tsis Siv Neeg Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., thiab cov extraction yuav tsum tau ua raws li kev qhia siv.Cov qauv muab rho tawm yog 200µL, thiab qhov pom zoo elution ntim yog 80µL.
Kev xaiv 3.
Pom zoo extraction reagent: QIAamp Viral RNA Mini Kit (52904) los ntawm QIAGEN los yog TIANamp Virus DNA/RNA Kit (YDP315-R), thiab cov extraction yuav tsum tau ua raws li nruj raws li cov lus qhia rau kev siv.Cov qauv muab rho tawm yog 140μL, thiab qhov pom zoo elution ntim yog 60μL.
