Mycobacterium Tuberculosis Nucleic Acid thiab Rifampicin Resistance
Khoom npe
HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid thiab Rifampicin Resistance Detection Kit (Melting Curve)
Daim ntawv pov thawj
CE
Kab mob sib kis
Mycobacterium tuberculosis, luv luv li Tubercle bacillus, TB, yog cov kab mob pathogenic uas ua rau tuberculosis.Tam sim no, cov tshuaj tiv thaiv kab mob ua ntej siv feem ntau suav nrog isoniazid, rifampicin thiab hexambutol, thiab lwm yam. Cov tshuaj tiv thaiv kab mob thib ob muaj xws li fluoroquinolones, amikacin thiab kanamycin, thiab lwm yam. Cov tshuaj tsim tshiab yog linezolid, bedaquiline thiab delamani, thiab lwm yam. . Txawm li cas los xij, vim yog siv cov tshuaj tiv thaiv kab mob tsis raug thiab cov yam ntxwv ntawm cov phab ntsa ntawm tes ntawm cov kab mob mycobacterium tuberculosis, mycobacterium tuberculosis tsim cov tshuaj tiv thaiv rau cov tshuaj tiv thaiv tuberculosis, uas ua rau muaj teeb meem loj rau kev tiv thaiv thiab kho tuberculosis.
Rifampicin tau siv dav hauv kev kho mob ntsws ntsws tuberculosis txij thaum xyoo 1970s, thiab muaj txiaj ntsig zoo.Nws tau yog thawj qhov kev xaiv los ua kom luv luv ntawm cov kws khomob ntawm cov neeg mob ntsws tuberculosis.Rifampicin tsis kam yog feem ntau tshwm sim los ntawm kev hloov pauv ntawm rpoB noob.Txawm hais tias cov tshuaj tiv thaiv kab mob tshiab tau tawm los tas li, thiab kev kho mob ntawm cov neeg mob ntsws ntsws ntsws kuj tseem txhim kho, tseem muaj cov txheeb ze tsis muaj cov tshuaj tiv thaiv kab mob, thiab qhov tshwm sim ntawm kev siv tshuaj irrational hauv kev kho mob kuj yog siab.Pom tseeb, Mycobacterium tuberculosis nyob rau hauv cov neeg mob pulmonary tuberculosis tsis tuaj yeem tua tag nrho raws sijhawm, uas nws thiaj li ua rau muaj kev sib txawv ntawm cov tshuaj tiv thaiv hauv tus neeg mob lub cev, ua rau lub sijhawm ntev ntawm tus kab mob, thiab ua rau muaj kev pheej hmoo ntawm kev tuag ntawm tus neeg mob.
Channel
| Channel | Channels thiab Fluorophores | Reaction Buffer A | Cov tshuaj tiv thaiv Buffer B | Reaction Buffer C |
| FAM Channel | Reporter: FAM, Quencher: Tsis yog | PEB 507-514 | PEB 513-520 | 38KD thiab IS6110 |
| CY5 Channel | Tus neeg sau xov xwm: CY5, Quencher: Tsis muaj | PEB 520-527 | PEB 527-533 | / |
| HEX (VIC) Channel | Tus neeg sau xov xwm: HEX (VIC), Quencher: Tsis muaj | Internal tswj | Internal tswj | Internal tswj |
Technical Parameters
| Cia | ≤-18 ℃ Hauv qhov tsaus ntuj |
| Txee lub neej | 12 hli |
| Hom Qauv | Cov hnoos qeev |
| CV | ≤ 5.0% |
| LoD | mycobacterium tuberculosis 50 kab mob / mL rifampicin-resistant qus hom: 2x103kab mob / ml homozygous mutant: 2 x 103kab mob / ml |
| Qhov tshwj xeeb | Nws kuaj pom cov kab mob mycobacterium tuberculosis qus thiab qhov chaw hloov pauv ntawm lwm cov tshuaj tiv thaiv kab mob xws li katG 315G> C\A, InhA-15C> T, cov txiaj ntsig kev kuaj pom tsis muaj kev tiv thaiv rau rifampicin, uas txhais tau hais tias tsis muaj kev sib cuam tshuam. |
| Cov cuab yeej siv tau: | SLAN-96P Real-Time PCR Systems BioRad CFX96 Real-Time PCR System LightCycler480® Real-Time PCR System |
Ua hauj lwm Flow
Yog siv Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los yog Macro & Micro-Test Viral DNA / RNA Column (HWTS-3022-50) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. 200μL ntawm Kev Tswj Xyuas Zoo, kev tswj tsis zoo thiab ua tiav cov hnoos qeev yuav tsum tau sim ua ntu zus, thiab ntxiv 10μL ntawm kev tswj sab hauv cais rau hauv Kev Tswj Xyuas Zoo, kev tswj tsis zoo thiab kev ua cov hnoos qeev kom raug kuaj, thiab cov kauj ruam tom ntej yuav tsum tau ua nruj me ntsis. raws li cov lus qhia rho tawm.Cov qauv muab rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 100μL.
