Kev Hloov Pauv ntawm Tib Neeg BRAF Gene V600E
Lub npe khoom
HWTS-TM007-Tib Neeg BRAF Gene V600E Kev Tshawb Fawb Txog Kev Hloov Pauv (Fluorescence PCR)
Daim Ntawv Pov Thawj
CE/TFDA
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Muaj ntau tshaj 30 hom kev hloov pauv BRAF tau pom, uas kwv yees li 90% nyob hauv exon 15, qhov twg V600E kev hloov pauv yog suav tias yog kev hloov pauv ntau tshaj plaws, uas yog, thymine (T) ntawm qhov chaw 1799 hauv exon 15 hloov pauv mus rau adenine (A), ua rau hloov pauv ntawm valine (V) ntawm qhov chaw 600 los ntawm glutamic acid (E) hauv cov khoom protein. Kev hloov pauv BRAF feem ntau pom muaj nyob rau hauv cov qog nqaij hlav phem xws li melanoma, mob qog nqaij hlav colorectal, mob qog nqaij hlav thyroid, thiab mob qog nqaij hlav ntsws. Kev nkag siab txog kev hloov pauv ntawm BRAF gene tau dhau los ua qhov xav tau los tshuaj xyuas EGFR-TKIs thiab BRAF gene mutation-targeted tshuaj hauv kev kho mob rau cov neeg mob uas tuaj yeem tau txais txiaj ntsig.
Channel
| FAM | Kev hloov pauv V600E, kev tswj hwm sab hauv |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | cov qauv ntaub so ntswg pathological uas tau muab tso rau hauv paraffin |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Siv cov khoom siv los ntes cov kev tswj xyuas zoo ntawm LoD. a) nyob rau hauv 3ng/μL keeb kwm yav dhau los qus, 1% kev hloov pauv tuaj yeem pom hauv cov tshuaj tiv thaiv buffer ruaj khov; b) nyob rau hauv 1% kev hloov pauv, kev hloov pauv ntawm 1 × 103Cov ntawv theej/mL nyob rau hauv keeb kwm yav dhau los ntawm 1 × 105Cov ntawv theej/mL tuaj yeem pom tau ruaj khov hauv lub buffer tshuaj tiv thaiv; c) IC Reaction Buffer tuaj yeem pom qhov kev tswj xyuas zoo qis tshaj plaws SW3 ntawm lub tuam txhab kev tswj hwm sab hauv. |
| Cov Cuab Yeej Siv Tau: | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub SijhawmApplied Biosystems 7300 Real-Time PCR Cov Txheej Txheem, QuantStudio® 5 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Lub tshuab LightCycler®480 Real-Time PCR BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagents uas pom zoo kom rho tawm: QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) uas Tiangen Biotech (Beijing) Co., Ltd. tsim tawm.
