Tib Neeg Braf Gene V600E Kev Sib Tsuag
Khoom npe
HWTS Kev Siv TM007
Daim ntawv muaj cai
CE / TFDA
Kev kawm
Ntau dua 30 hom kev sib hloov ntawm Braf tau pom, uas muaj txog 90% yog suav tias yog qhov kev hloov pauv ntau tshaj plaws, uas yog, thymine (t) ntawm txoj hauj lwm 1799 hauv exon 15 yog mutated rau Adenine (a), ua rau hloov chaw ntawm Valine (V) ntawm txoj hauj lwm 600 los ntawm Glutamic Acid (E) hauv cov khoom protein. Kev sib hloov ntawm cov qog ua cov qog ua kom pom tseeb xws li melanoma, cancere cancer, cov qog nqaij hlav cancer, thiab mob ntsws. Nkag siab txog kev sib hloov ntawm Braf Gene tau dhau los ua qhov kev xav tau ntawm EGR-TKIS thiab BRFAr gene kev kho tshuaj rau cov neeg mob uas tuaj yeem tau txais txiaj ntsig.
Tshooj
| Tshaib plab | V600E mutation, sab hauv |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ |
| Txee-Lub Neej | 9 lub hlis |
| Zauv Hom | Paraffin-embedded patholog cov ntaub so ntswg kuaj |
| CV | <0% |
| Ct | ≤38 |
| Lub lab | Siv cov khoom siv los kuaj pom cov lod zoo tswj. a) Nyob rau hauv 3ng / μl qus-hom keeb kwm yav dhau, 1% kev sib hloov pauv tuaj yeem kuaj pom hauv cov tshuaj tiv thaiv buffer stably; b) nyob rau hauv 1% kev sib hloov pauv, kev hloov pauv ntawm 1 × 103Luam / ML hauv qus-hom tsiaj qus ntawm 1 × 105Cov ntawv luam / ml tuaj yeem raug ntes tau rau hauv cov tshuaj tiv thaiv tsis; c) Lub icor tiv thaiv buffer tuaj yeem kuaj qhov ntsuas qis tshaj plaws txwv tsis pub muaj qhov zoo SW3 ntawm lub tuam txhab kev tswj hwm sab hauv. |
| Muaj cov cuab yeej siv: | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuabSiv biosystems 7300 Real-Tim PCR Tshuab, QuatStudio® 5 REO-lub sijhawm PRR TSHUAB Lightcycler ®480 REO-SIJHAWM PCR system Biorad Cfx96 REO-SIJHAWM PCR system |
Ua Haujlwm Ntws
Pom zoo rho tawm cov khoom siv: Qiabe Qiap DNA FFPE Cov Khoom Siv Nqaij (56404), Beijing Biotech (Beijing) Co., Ltd.
