Tib neeg CYP2C19 Gene Polymorphism
Khoom npe
HWTS-GE012A-Human CYP2C19 Gene Polymorphism Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE / TSO
Kab mob sib kis
CYP2C19 yog ib qho ntawm cov tshuaj tseem ceeb metabolizing enzymes hauv tsev neeg CYP450.Ntau yam endogenous substrates thiab txog 2% ntawm cov tshuaj kho mob yog metabolized los ntawm CYP2C19, xws li cov metabolism hauv antiplatelet aggregation inhibitors (xws li clopidogrel), proton twj tso kua mis inhibitors (omeprazole), anticonvulsants, thiab lwm yam. yam tshuaj.Cov kev hloov pauv no ntawm * 2 (rs4244285) thiab * 3 (rs4986893) ua rau poob ntawm cov enzyme kev ua los ntawm CYP2C19 noob thiab qhov tsis muaj zog ntawm kev muaj peev xwm metabolic substrate, thiab nce ntshav concentration, thiaj li ua rau muaj kev phiv tshuaj cuam tshuam nrog ntshav concentration.* 17 (rs12248560) tuaj yeem ua kom cov enzyme kev ua tau zoo los ntawm CYP2C19 noob, tsim cov metabolites nquag, thiab txhim kho cov platelet aggregation inhibition thiab ua rau muaj kev pheej hmoo los ntshav.Rau cov neeg uas muaj cov metabolism hauv qeeb qeeb, noj cov tshuaj ib txwm muaj rau lub sijhawm ntev yuav ua rau muaj kev phom sij loj thiab cov kev mob tshwm sim: feem ntau yog daim siab puas, hematopoietic system puas, hauv nruab nrab paj hlwb puas, thiab lwm yam, uas tuaj yeem ua rau tuag taus.Raws li tus neeg sib txawv ntawm cov tshuaj metabolism, nws feem ntau muab faib ua plaub phenotypes, uas yog ultra-ceev metabolism (UM, * 17 / * 17, * 1 / * 17), ceev metabolism (RM, * 1 / * 1). ), cov metabolism hauv nruab nrab (IM, * 1 / * 2, * 1 / * 3), qeeb metabolism (PM, * 2 / * 2, * 2 / * 3, * 3 / * 3).
Channel
| FAM | CYP2C19*2 |
| CY 5 | CYP2C9*3 |
| ROX | CYP2C19*17 |
| VIC/HEX | IC |
Technical Parameters
| Cia | Ua kua: ≤-18 ℃ |
| Txee lub neej | 12 hli |
| Hom Qauv | Fresh EDTA anticoagulated ntshav |
| CV | ≤ 5.0% |
| LoD | 1.0 ng / μL |
| Qhov tshwj xeeb | Tsis muaj qhov cuam tshuam nrog lwm cov kab ke sib xws (CYP2C9 gene) hauv tib neeg genome.Kev hloov pauv ntawm CYP2C19 * 23, CYP2C19 * 24 thiab CYP2C19 * 25 qhov chaw sab nraum qhov kev tshawb pom ntawm cov khoom siv no tsis muaj kev cuam tshuam rau kev tshawb nrhiav cov khoom siv no. |
| Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Pom zoo extraction reagent: Macro & Micro-Test General DNA / RNA Cov Khoom Siv (HWTS-3019) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kev rho tawm yuav tsum tau muab rho tawm raws li cov lus qhia.Cov qauv rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 100μL.
Pom zoo extraction reagent: Wizard® Genomic DNA Purification Kit (Catalog No.: A1120) los ntawm Promega, Nucleic Acid Extraction lossis Purification Reagent (YDP348) los ntawm Tiangen Biotech (Beijing) Co., Ltd.yuav tsum tau muab rho tawm raws li cov lus qhia extraction, thiab qhov pom zoo extraction ntim yog 200 μL thiab qhov pom zoo elution ntim yog 160 μL.
