Tib neeg cytomeGalovirus (HCMV) nucleic acid

Cov lus piav qhia luv luv:

Cov khoom siv no yog siv rau kev txiav txim siab zoo ntawm nucleic acids hauv cov qauv nrog cov ntshav lossis mob ntshav los ntawm tus mob HCMV, yog li pab kuaj mob HCMV.

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Khoom Qhia Qhia

Khoom Ntawv

Khoom npe

HWTS-ur008A-tib neeg cytomeGalovirus (HCMV) nucleic acid nrhiav tau khoom siv (fluorescence pcr)

Kev kawm

Tib neeg cytomeGalovirus (HCMV) yog ib tus tswv cuab nrog genome loj tshaj plaws hauv tsev neeg tus kab mob thiab tsev neeg tuaj yeem encode ntau tshaj 200 proteins. HCMV yog nqaim txwv nyob rau hauv nws tus tswv tsev rau tib neeg, thiab tseem tsis muaj tsiaj ntawm nws tus mob. HCMV muaj qhov qeeb thiab ntev replication lub voj voog los ua ib qho tsim cov khoom siv hauv kev tso pa hauv lub cev, thiab cytoplasmic lub cev thiab o ntawm tes (giant hlwb), yog li lub npe. Raws li cov heterogeneity ntawm nws genome thiab phenotype, HCMV tuaj yeem muab faib ua ntau hom kev sib txawv, uas, tsis yog qhov tseem ceeb hauv tsev kho mob.

Tus kab mob HCMV yog qhov mob hauv lub cev, uas muaj ntau yam kabmob, muaj ntau yam kev ua si los tsim cov kabmob hauv nruab nrog, thiab tuaj yeem ua rau muaj ntau yam kev mob siab, thiab tuaj yeem ua rau muaj ntau yam kev mob siab, mob plab, thiab thrombocytopenic purpura. Tus kab mob HCMV yog qhov tshwm sim thiab zoo li kis thoob ntiaj teb. Nws yog ntau kawg hauv cov pejxeem, nrog qhov xwm txheej ntawm 45-50% thiab ntau dua 90% hauv kev tsim thiab tsim cov teb chaws, feem. HCMV tuaj yeem nteg dormant hauv lub cev ntev. Thaum lub cev tiv thaiv tsis muaj zog, tus kab mob yuav qhib kom muaj kab mob, thiab tuaj yeem hloov kho cov neeg mob necrosxy thiab phom sij rau cov neeg mob lub neej mob hnyav. Ntxiv rau cov tseemceeb, miscariage thiab tus me nyuam ua ntej ntawm cov kab mob hauv kev sib tw, yog li HCMV kis tau muaj peev xwm cuam tshuam rau kev saib xyuas ua ntej thiab cov pej xeem zoo.

Tshooj

Tshaib plab	HCMV DNAN
Vic (Hex)	Sab hauv tswj

Kev tsis muaj

Chaw ntim khoom	Cov kua: ≤-18 ℃ hauv kev tsaus ntuj
Txee-Lub Neej	12 lub hlis
Zauv Hom	Seem cov qauv, cov qauv ntshav, ntshav piv txwv
Ct	≤38
CV	APR5.0%
Lub lab	50 daim ntawv luam tawm / tshuaj tiv thaiv
Yam tshwj xeeb	Tsis muaj tus ntoo khaub lig-reactivity nrog tus kab mob siab B mob, Herpes yooj yim tus kab mob Hom 2, tib neeg cov qauv kuaj pom, thiab lwm yam.
Muaj cov cuab yeej siv:	Nws tuaj yeem phim rau lub chaw ua yeeb yaj kiab loj flu inorescent ntawm lub lag luam. Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p ROOST-SICTICE PRRY Lightcycler ®480 REO-SIJHAWM PCR system LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEV Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System

Ua Haujlwm Ntws

HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv cov kua roj-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-xeem muaj metraction yuav tsum tau muab rho tawm raws li cov lus qhia. Cov kev rho tawm cov qauv ntim yog 200μL, thiab cov lus pom zoo kom meej meej ntim yog 80μL.

Pom zoo rho tawm reagent: Qiaamp DNA Mini Khoom (51304), nucleic acid extraction lossis purification reident (ydp315) los ntawm Tiangen Biotech (Beijing) Co., Ltd. Yuav tsum tau muab rho tawm raws li cov lus qhia rho tawm, thiab cov lus pom zoo yuav tsum muaj zog muaj 200 μl thiab cov lus pom zoo kom pom zoo kom pom zoo yog 100 μL.

Yav dhau los: Mycobacacterium tuberculosis nucleic acid thiab rifampicin tsis kam

Tom ntej: Tus kab mob ua pa syncatators antigen

Sau koj cov lus ntawm no thiab xa mus rau peb