Human Cytomegalovirus (HCMV) Nucleic Acid
Khoom npe
HWTS-UR008A-Human cytomegalovirus (HCMV) nucleic acid kuaj cov khoom (Fluorescence PCR)
Kab mob sib kis
Tib neeg cytomegalovirus (HCMV) yog ib tug tswv cuab nrog cov genome loj tshaj plaws hauv tsev neeg tus kab mob herpes thiab tuaj yeem nkag mus rau ntau tshaj 200 proteins.HCMV yog nqaim txwv nyob rau hauv nws tus tswv tsev ntau yam rau tib neeg, thiab tseem tsis muaj tsiaj qauv ntawm nws tus kab mob.HCMV muaj lub voj voog qeeb thiab ntev replication los tsim ib lub cev intranuclear inclusion lub cev, thiab ua rau zus tau tej cov perinuclear thiab cytoplasmic inclusion lub cev thiab cell o (cov hlwb loj), li no lub npe.Raws li qhov heterogeneity ntawm nws cov genome thiab phenotype, HCMV tuaj yeem muab faib ua ntau hom kab mob, ntawm cov uas muaj qee yam kev hloov pauv ntawm cov tshuaj antigenic, uas, txawm li cas los xij, tsis muaj qhov tseem ceeb hauv kev kho mob.
Tus kab mob HCMV yog ib qho kab mob hauv lub cev, uas nyob hauv tsev kho mob muaj ntau yam kabmob, muaj cov tsos mob sib txawv, feem ntau nyob twj ywm, thiab tuaj yeem ua rau ob peb tus neeg mob tsim cov kab mob ntau yam xws li retinitis, kab mob siab, mob ntsws, mob ntsws, mob plab, monocytosis, thiab thrombocytopenic. purpura.Tus kab mob HCMV muaj ntau heev thiab zoo li kis thoob ntiaj teb.Nws muaj ntau heev nyob rau hauv cov pej xeem, nrog rau qhov tshwm sim ntawm 45-50% thiab ntau tshaj 90% nyob rau hauv tsim thiab tsim lub teb chaws, feem.HCMV tuaj yeem pw tsaug zog hauv lub cev ntev.Thaum lub cev tiv thaiv kab mob tsis muaj zog, tus kab mob no yuav raug qhib kom ua rau cov kab mob, tshwj xeeb tshaj yog cov kab mob rov tshwm sim hauv cov neeg mob leukemia thiab cov neeg mob hloov pauv, thiab tuaj yeem ua rau lub cev hloov pauv hauv lub cev necrosis thiab ua rau muaj kev phom sij rau cov neeg mob lub neej thaum mob hnyav.Ntxiv nrog rau kev yug menyuam, nchuav menyuam thiab yug ntxov ntxov los ntawm kev kis kab mob hauv plab, cytomegalovirus tuaj yeem ua rau muaj qhov tsis zoo, yog li tus kab mob HCMV tuaj yeem cuam tshuam rau kev saib xyuas ua ntej yug menyuam thiab tom qab yug menyuam thiab cov pejxeem zoo.
Channel
FAM | HCMV DNA |
VIC (HEX) | Internal Control |
Technical Parameters
Cia | Ua kua: ≤-18 ℃ Hauv tsaus |
Txee lub neej | 12 hli |
Hom Qauv | Kev kuaj ntshav, Plasma Sample |
Ct | ≤38 |
CV | ≤ 5.0% |
LoD | 50 Daim ntawv / cov lus teb |
Qhov tshwj xeeb | Tsis muaj kev sib cuam tshuam nrog tus kab mob siab B, kab mob siab C, kab mob tib neeg papillomavirus, herpes simplex virus type 1, herpes simplex virus type 2, normal human serum test, etc. |
Cov cuab yeej siv tau: | Nws tuaj yeem phim cov khoom siv hluav taws xob fluorescent PCR hauv khw. Siv Biosystems 7500 Real-Time PCR Systems Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Kev rho tawm yuav tsum tau muab rho tawm raws li cov lus qhia.Cov qauv rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 80μL.
Pom zoo extraction reagent: QIAamp DNA Mini Kit (51304), Nucleic Acid Extraction lossis Purification Reagent (YDP315) los ntawm Tiangen Biotech (Beijing) Co., Ltd.yuav tsum tau muab rho tawm raws li cov lus qhia extraction, thiab qhov pom zoo extraction ntim yog 200 μL thiab qhov pom zoo elution ntim yog 100 μL.