Kev Hloov Pauv ntawm Tib Neeg EGFR Gene 29
Lub npe khoom
HWTS-TM0012A-Tib Neeg EGFR Gene 29 Kev Tshawb Fawb Txog Kev Hloov Pauv (Fluorescence PCR)
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Mob qog noj ntshav hauv lub ntsws tau dhau los ua qhov ua rau muaj kev tuag ntau tshaj plaws thoob ntiaj teb, ua rau tib neeg muaj kev phom sij heev rau kev noj qab haus huv. Mob qog noj ntshav hauv lub ntsws uas tsis yog me me muaj li ntawm 80% ntawm cov neeg mob qog noj ntshav hauv lub ntsws. EGFR tam sim no yog lub hom phiaj tseem ceeb tshaj plaws rau kev kho mob qog noj ntshav hauv lub ntsws uas tsis yog me me. Kev phosphorylation ntawm EGFR tuaj yeem txhawb kev loj hlob ntawm cov hlwb qog, kev sib txawv, kev nkag mus, metastasis, tiv thaiv apoptosis, thiab txhawb kev loj hlob ntawm cov hlwb qog. EGFR tyrosine kinase inhibitors (TKI) tuaj yeem thaiv txoj kev teeb tsa EGFR los ntawm kev txwv tsis pub EGFR autophosphorylation, yog li ntawd txwv tsis pub kev loj hlob thiab kev sib txawv ntawm cov hlwb qog, txhawb kev loj hlob ntawm cov hlwb qog, txo cov hlwb qog, thiab lwm yam, kom ua tiav kev kho mob rau cov hlwb qog. Muaj ntau txoj kev tshawb fawb tau qhia tias kev kho mob zoo ntawm EGFR-TKI muaj feem cuam tshuam nrog qhov xwm txheej ntawm EGFR gene mutation, thiab tuaj yeem tshwj xeeb txwv tsis pub kev loj hlob ntawm cov hlwb qog nrog EGFR gene mutation. Lub EGFR gene nyob ntawm caj npab luv luv ntawm chromosome 7 (7p12), nrog tag nrho ntev ntawm 200Kb thiab muaj 28 exons. Thaj chaw hloov pauv feem ntau nyob hauv exons 18 txog 21, codons 746 txog 753 kev hloov pauv ntawm exon 19 suav txog li 45% thiab L858R kev hloov pauv ntawm exon 21 suav txog li 40% txog 45%. Cov Lus Qhia ntawm NCCN rau Kev Kuaj Mob thiab Kev Kho Mob Cancer Ntsws Tsis Me Me hais meej meej tias kev kuaj mob hloov pauv ntawm EGFR yog qhov yuav tsum tau ua ntej kev tswj hwm EGFR-TKI. Cov khoom siv kuaj mob no yog siv los coj kev tswj hwm ntawm cov tshuaj epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), thiab muab lub hauv paus rau kev kho mob tus kheej rau cov neeg mob uas muaj mob qog nqaij hlav tsis me me. Cov khoom siv no tsuas yog siv rau kev kuaj pom cov kev hloov pauv ntau hauv EGFR gene hauv cov neeg mob uas muaj mob qog nqaij hlav tsis me me. Cov txiaj ntsig ntawm kev xeem tsuas yog rau kev siv hauv kev kho mob xwb thiab yuav tsum tsis txhob siv ua lub hauv paus rau kev kho mob tus kheej ntawm cov neeg mob. Cov kws kho mob yuav tsum xav txog tus neeg mob tus mob, cov cim qhia tshuaj, thiab kev kho mob. Cov tshuaj tiv thaiv thiab lwm yam cim qhia kev kuaj mob hauv chaw kuaj mob thiab lwm yam yog siv los txiav txim siab txog cov txiaj ntsig ntawm kev xeem.
Channel
| FAM | IC Reaction Buffer, L858R Reaction Buffer, 19del Reaction Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | Kua: ≤-18 ℃ Hauv qhov tsaus ntuj; Lyophilized: ≤30 ℃ Hauv qhov tsaus ntuj |
| Lub sijhawm khaws cia | Kua: 9 lub hlis; Lyophilized: 12 lub hlis |
| Hom Qauv | cov nqaij mob tshiab, seem mob khov, cov nqaij mob lossis seem uas muaj paraffin nyob hauv, plasma lossis serum |
| CV | <5.0% |
| LoD | Kev kuaj pom cov tshuaj tiv thaiv nucleic acid nyob rau hauv keeb kwm yav dhau ntawm 3ng / μL hom qus, tuaj yeem ntes tau 1% kev hloov pauv sai |
| Kev Tshwj Xeeb | Tsis muaj kev sib tshuam nrog cov DNA genomic tib neeg qus thiab lwm hom mutant |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub SijhawmApplied Biosystems 7300 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Cov Txheej Txheem PCR QuantStudio® 5 Real-Time Lub tshuab LightCycler® 480 Real-Time PCR BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
