Kev Hloov Pauv ntawm Tib Neeg EML4-ALK Fusion Gene
Lub npe khoom
HWTS-TM006-Tib Neeg EML4-ALK Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Daim Ntawv Pov Thawj
TFDA
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Cov khoom siv no yog siv los nrhiav 12 hom kev hloov pauv ntawm EML4-ALK fusion gene hauv cov qauv ntawm cov neeg mob qog nqaij hlav ntsws uas tsis yog me me hauv vitro. Cov txiaj ntsig ntawm kev xeem tsuas yog rau kev siv hauv kev kho mob xwb thiab yuav tsum tsis txhob siv ua lub hauv paus rau kev kho mob rau tus kheej ntawm cov neeg mob. Cov kws kho mob yuav tsum txiav txim siab txog cov txiaj ntsig ntawm kev xeem raws li cov yam xws li tus neeg mob tus mob, cov cim qhia txog tshuaj, kev teb rau kev kho mob, thiab lwm yam cim qhia txog kev kuaj hauv chaw kuaj mob. Qog nqaij hlav ntsws yog qog nqaij hlav phem tshaj plaws thoob ntiaj teb, thiab 80% ~ 85% ntawm cov neeg mob yog qog nqaij hlav ntsws uas tsis yog me me (NSCLC). Kev sib xyaw ntawm cov noob caj noob ces ntawm echinoderm microtubule-associated protein-like 4 (EML4) thiab anaplastic lymphoma kinase (ALK) yog lub hom phiaj tshiab hauv NSCLC, EML4 thiab ALK nyob rau hauv tib neeg P21 thiab P23 bands ntawm chromosome 2 thiab sib cais los ntawm kwv yees li 12.7 lab tus khub. Muaj tsawg kawg yog 20 qhov kev hloov pauv fusion tau pom, ntawm cov uas 12 qhov kev hloov pauv fusion hauv Rooj 1 yog qhov sib xws, qhov twg mutant 1 (E13; A20) yog qhov feem ntau, ua raws li mutants 3a thiab 3b (E6; A20), suav txog li 33% thiab 29% ntawm cov neeg mob uas muaj EML4-ALK fusion gene NSCLC, feem. ALK inhibitors sawv cev los ntawm Crizotinib yog cov tshuaj me me uas tsim rau ALK gene fusion mutations. Los ntawm kev txwv tsis pub ua haujlwm ntawm thaj tsam ALK tyrosine kinase, thaiv nws txoj kev taw qhia tsis zoo, yog li ntawd txwv tsis pub loj hlob ntawm cov hlwb qog, kom ua tiav kev kho mob rau cov qog. Cov kev tshawb fawb hauv tsev kho mob tau qhia tias Crizotinib muaj tus nqi zoo dua 61% hauv cov neeg mob uas muaj EML4-ALK fusion mutations, thaum nws yuav luag tsis muaj txiaj ntsig rau cov neeg mob qus. Yog li ntawd, kev kuaj pom ntawm EML4-ALK fusion mutation yog lub hauv paus thiab lub hauv paus rau kev coj siv cov tshuaj Crizotinib.
Channel
| FAM | Cov tshuaj tiv thaiv 1, 2 |
| VIC (HEX) | Cov tshuaj tiv thaiv 2 |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | ≤-18 ℃ |
| Lub sijhawm khaws cia | 9 lub hlis |
| Hom Qauv | cov qauv ntawm cov ntaub so ntswg lossis ntu uas muaj paraffin nyob hauv |
| CV | <5.0% |
| Ct | ≤38 |
| LoD | Cov khoom siv no tuaj yeem ntes tau kev hloov pauv fusion tsawg li 20 daim ntawv theej. |
| Cov Cuab Yeej Siv Tau: | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev SLAN ®-96P Cov Txheej Txheem PCR Lub Sijhawm Tiag QuantStudio™ 5 Cov Txheej Txheem PCR Lub Sijhawm Tiag Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagent uas pom zoo kom rho tawm: RNeasy FFPE Kit (73504) los ntawm QIAGEN, Paraffin-embedded Tissue Sections Total RNA Extraction Kit (DP439) los ntawm Tiangen Biotech (Beijing) Co., Ltd.
