Tib neeg Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene
Khoom npe
HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE
Kab mob sib kis
Hauv cov neeg laus, ntau tshaj 10 8 lub plab hnyuv epithelial poob tawm ntawm lub plab hnyuv phab ntsa txhua hnub, thiab raug tso tawm nrog cov quav los ntawm txoj hnyuv loj peristalsis.Vim tias cov qog hlwb feem ntau yuav poob tawm ntawm txoj hnyuv ntawm qhov txawv txav, cov quav ntawm cov neeg mob qog nqaij hlav muaj ntau cov kab mob thiab cov kab mob txawv txav ntawm tes, uas yog cov khoom hauv paus rau kev tshawb nrhiav cov quav ruaj khov.Cov kev tshawb fawb tau pom tias kev hloov kho methylation ntawm cov neeg txhawb nqa cov noob yog ib qho tshwm sim thaum ntxov hauv cov qog nqaij hlav, thiab cov khoom siv caj ces tau los ntawm cov quav hauv cov neeg mob qog noj ntshav tuaj yeem cuam tshuam txog kev mob qog noj ntshav hauv cov hnyuv ua ntej.
NDRG4, tseem hu ua SMAP-8 thiab BDM1, yog ib qho ntawm plaub tus tswv cuab ntawm NDRG noob tsev neeg (NDRG1-4), uas tau pom tias cuam tshuam nrog kev loj hlob ntawm tes, sib txawv, kev loj hlob thiab kev ntxhov siab.Nws tau raug txheeb xyuas tias NDRG4 methylation yog qhov muaj peev xwm biomarker rau kev kuaj pom tsis muaj kab mob qog noj ntshav hauv cov quav.
SEPT9 yog ib tug tswv cuab ntawm tsev neeg Septin noob, uas muaj tsawg kawg yog 13 noob caj noob ces encode ib tug conserved GTPase domain uas tuaj yeem khi cytoskeleton cov proteins uas muaj feem xyuam nrog kev faib cell thiab qog nqaij hlav.Cov kev tshawb fawb tau pom tias cov methylated Septin9 cov noob cov ntsiab lus yog cov yam ntxwv nce hauv cov quav kuaj los ntawm cov neeg mob qog nqaij hlav hauv plab.
Secreted frizzled-related proteins (sFRPs) yog soluble proteins uas yog ib chav kawm ntawm Wnt txoj kev antagonists vim lawv cov yam ntxwv siab homology rau frizzled (Fz) receptor rau Wnt signaling.Inactivation ntawm SFRP noob ua rau tsis muaj kev tswj hwm ntawm Wnt signaling txuam nrog mob qog noj ntshav.Tam sim no, SFRP2 methylation nyob rau hauv cov quav tuaj yeem siv los ua cov biomarker uas tsis muaj kev cuam tshuam rau kev kuaj mob qog noj ntshav.
BMP3 yog ib tug tswv cuab ntawm TGF-B superfamily thiab yog li ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm embryonic los ntawm inducing thiab shaping ntxov pob txha tsim.BMP3 yog hypermethylated hauv mob qog noj ntshav thiab tuaj yeem siv los ua tus cim qog nqaij hlav tseem ceeb.
SDC2 yog lub cell nto heparan sulfate proteoglycan koom nrog hauv kev tswj hwm ntawm ntau cov txheej txheem physiological thiab pathological.Cov txheej txheem lub cev muaj xws li cell proliferation, sib txawv, adhesion, cytoskeletal lub koom haum, tsiv teb tsaws, qhov txhab kho, cell-matrix kev sib txuas lus, angiogenesis;Cov txheej txheem pathological muaj xws li mob thiab mob qog noj ntshav.Qib methylation ntawm SDC2 noob nyob rau hauv cov ntaub so ntswg mob qog noj ntshav tau ntau dua li cov ntaub so ntswg.
Channel
Cov tshuaj tiv thaiv kab mob A | VIC/HEX | methylated NDRG4 noob |
ROX | methylated SEPT9 noob | |
CY 5 | tswj sab hauv | |
Reaction tsis B | VIC/HEX | methylated SFRP2 noob |
ROX | methylated BMP3 noob | |
FAM | methylated SDC2 noob | |
CY 5 | tswj sab hauv |
Txhais lus
Gene | Teeb liab Channel | Ct Tus nqi | Txhais lus |
NDRG 4 | VIC (HEX) | Tus nqi Ct≤38 | NDRG4 positive |
Tus nqi Ct> 38 lossis unde | NDRG4 negative | ||
SIB 9 | ROX | Tus nqi Ct≤38 | SEPT9 zoo |
Tus nqi Ct> 38 lossis unde | SEPT9 negative | ||
SFRP2 | VIC (HEX) | Tus nqi Ct≤38 | SFRP2 zoo |
Tus nqi Ct> 38 lossis unde | SFRP2 tsis zoo |
Technical Parameters
Cia | Ua kua: ≤-18 ℃ |
Txee lub neej | 9 muaj |
Hom Qauv | Stool Sample |
CV | ≤ 5.0% |
Qhov tshwj xeeb | Tsis muaj kev sib cuam tshuam nrog rau daim siab mob qog noj ntshav, mob qog noj ntshav duct, thyroid cancer thiab mob ntsws cancer |
Cov cuab yeej siv tau | QuantStudio ®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler |
Ua hauj lwm Flow
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS- 3006).