Human ROS1 Fusion Gene Mutation
Khoom npe
HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Daim ntawv pov thawj
CE
Kab mob sib kis
ROS1 yog transmembrane tyrosine kinase ntawm tsev neeg insulin receptor.ROS1 fusion noob tau raug lees paub tias yog lwm yam tseem ceeb uas tsis yog me me ntawm lub ntsws qog nqaij hlav qog noj ntshav.Raws li ib tug neeg sawv cev ntawm ib tug tshiab tshwj xeeb molecular subtype, qhov tshwm sim ntawm ROS1 fusion gene nyob rau hauv NSCLC Kwv yees li 1% mus rau 2% ROS1 mas undergoes gene rearrangement nyob rau hauv nws exons 32, 34, 35 thiab 36. Tom qab nws yog fused nrog noob xws li CD74, EZR, SLC34A2, thiab SDC4, nws yuav txuas ntxiv mus qhib ROS1 tyrosine kinase cheeb tsam.ROS1 kinase ua haujlwm tsis zoo tuaj yeem ua rau cov kev taw qhia qis qis xws li RAS / MAPK / ERK, PI3K / Akt / mTOR, thiab JAK3 / STAT3, yog li koom nrog kev loj hlob, sib txawv thiab metastasis ntawm cov qog hlwb, thiab ua rau mob qog noj ntshav.Ntawm ROS1 fusion kev hloov pauv, CD74-ROS1 suav txog li 42%, EZR suav txog li 15%, SLC34A2 suav txog li 12%, thiab SDC4 suav txog li 7%.Cov kev tshawb fawb tau pom tias ATP-binding site ntawm catalytic domain ntawm ROS1 kinase thiab ATP-binding site ntawm ALK kinase muaj homology txog li 77%, yog li ALK tyrosine kinase me me molecule inhibitor crizotinib thiab thiaj li muaj cov nyhuv curative. nyob rau hauv kev kho mob ntawm NSCLC nrog fusion mutation ntawm ROS1.Yog li ntawd, kev tshawb pom ntawm ROS1 fusion kev hloov pauv yog qhov tsim nyog thiab lub hauv paus rau kev taw qhia kev siv tshuaj crizotinib.
Channel
| FAM | Reaction Buffer 1, 2, 3 thiab 4 |
| VIC (HEX) | Reaction Buffer 4 |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 9 muaj |
| Hom Qauv | paraffin-embedded pathological ntaub so ntswg los yog hlais cov qauv |
| CV | 5.0% |
| Ct | ≤38 |
| LoD | Cov khoom siv no tuaj yeem txheeb xyuas qhov kev hloov pauv fusion tsawg li 20 daim ntawv. |
| Cov cuab yeej siv tau: | Siv Biosystems 7500 Real-Time PCR SystemsSiv Biosystems 7500 Fast Real-Time PCR Systems SLAN ®-96P Real-Time PCR Systems QuantStudio™ 5 Real-Time PCR Systems LightCycler® 480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
Pom zoo extraction reagent: RNeasy FFPE Kit (73504) los ntawm QIAGEN, Paraffin Embedded Tissue Section Total RNA Extraction Kit (DP439) los ntawm Tiangen Biotech (Beijing) Co., Ltd.
