Mycobacterium Tuberculosis Isoniazid Resistance Mutation
Khoom npe
HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)
Kab mob sib kis
Mycobacterium tuberculosis, luv luv li Tubercle bacillus (TB), yog cov kab mob pathogenic uas ua rau tuberculosis.Tam sim no, cov tshuaj tiv thaiv kab mob ua ntej siv feem ntau suav nrog isoniazid, rifampicin thiab hexambutol, thiab lwm yam. Cov tshuaj tiv thaiv kab mob thib ob muaj xws li fluoroquinolones, amikacin thiab kanamycin, thiab lwm yam. Cov tshuaj tsim tshiab yog linezolid, bedaquiline thiab delamani, thiab lwm yam. . Txawm li cas los xij, vim yog siv cov tshuaj tiv thaiv kab mob tsis raug thiab cov yam ntxwv ntawm cov phab ntsa ntawm tes ntawm cov kab mob mycobacterium tuberculosis, mycobacterium tuberculosis tsim cov tshuaj tiv thaiv rau cov tshuaj tiv thaiv tuberculosis, uas ua rau muaj teeb meem loj rau kev tiv thaiv thiab kho tuberculosis.
Channel
FAM | MP nucleic acid |
ROX | Internal Control |
Technical Parameters
Cia | ≤-18 ℃ |
Txee lub neej | 12 hli |
Hom Qauv | hnoos qeev |
CV | ≤ 5% |
LoD | Qhov kev tshawb pom txwv rau cov kab mob qus-hom isoniazid resistant yog 2x103 cov kab mob / mL, thiab qhov kev kuaj pom cov kab mob mutant yog 2x103 kab mob / mL. |
Qhov tshwj xeeb | a.Tsis muaj cov tshuaj tiv thaiv ntawm tib neeg genome, lwm yam nontuberculous mycobacteria thiab kab mob ntsws uas kuaj pom los ntawm cov khoom siv no.b.Cov chaw hloov pauv ntawm lwm cov tshuaj tiv thaiv kab mob hauv cov tsiaj qus Mycobacterium tuberculosis, xws li kev tiv thaiv kev txiav txim siab thaj tsam ntawm rifampicin rpoB noob, tau kuaj pom, thiab cov txiaj ntsig kev sim pom tias tsis muaj kev tiv thaiv rau isoniazid, qhia tias tsis muaj kev sib cuam tshuam. |
Cov cuab yeej siv tau | SLAN-96P Real-Time PCR SystemsBioRad CFX96 Real-Time PCR Systems LightCycler480® Real-Time PCR System |
Ua hauj lwm Flow
Yog siv Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. rau kev rho tawm, ntxiv 200μL ntawm cov kev tswj tsis zoo thiab ua cov hnoos qeev kom raug kuaj ua ntu zus, thiab ntxiv 10μL ntawm kev tswj xyuas sab hauv nyias mus rau hauv kev tswj tsis zoo, ua tiav cov hnoos qeev los kuaj, thiab cov kauj ruam tom ntej yuav tsum tau ua nruj me ntsis raws li cov lus qhia extraction.Qhov ntim tau muab rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 100μL.