Mycobacterium Tuberculosis Nucleic Acid thiab Rifampicin (RIF), Resistance (INH)

Lus piav qhia luv luv:

Cov khoom siv no yog siv rau hauv vitro qualitative detection ntawm Mycobacterium tuberculosis DNA nyob rau hauv tib neeg hnoos qeev, khoom kab lis kev cai (LJ Medium) thiab kua kab lis kev cai (MGIT Medium), bronchial lavage kua, thiab cov kev hloov nyob rau hauv lub 507-533 amino acid codon cheeb tsam (81bp, rifampicin resistance lub cheeb tsam ntawm mycobacteria) rifampicin tsis kam, nrog rau kev hloov pauv hauv qhov chaw hloov pauv tseem ceeb ntawm Mycobacterium tuberculosis isoniazid resistance.Nws muab kev pabcuam rau kev kuaj mob ntawm tus kab mob Mycobacterium tuberculosis, thiab nws kuaj pom cov noob tseem ceeb ntawm rifampicin thiab isoniazid, uas pab kom nkag siab txog cov tshuaj tiv thaiv ntawm Mycobacterium tuberculosis.

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HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid thiab Rifampicin (RIF), (INH) Cov Khoom Siv Ntsuas (Melting Curve)

Kab mob sib kis

Mycobacterium tuberculosis, luv luv li Tubercle bacillus (TB), yog cov kab mob pathogenic uas ua rau tuberculosis, thiab tam sim no, feem ntau siv thawj kab tshuaj tiv thaiv kab mob xws li isoniazid, rifampicin thiab ethambutol, thiab lwm yam.^[1]. Txawm li cas los xij, vim yog siv cov tshuaj tiv thaiv kab mob tsis raug thiab cov yam ntxwv ntawm cov phab ntsa ntawm tes ntawm mycobacterium tuberculosis nws tus kheej, mycobacterium tuberculosis tau tsim cov tshuaj tiv thaiv rau cov tshuaj tiv thaiv kab mob, thiab ib daim ntawv txaus ntshai tshwj xeeb yog multidrug-resistant tuberculosis (MDR-TB), uas tiv taus rau ob hom tshuaj feem ntau thiab siv tau zoo, thiab rifampic.^[2].

Qhov teeb meem ntawm tuberculosis tshuaj tiv thaiv muaj nyob rau hauv txhua lub teb chaws tshawb fawb los ntawm WHO. Txhawm rau muab cov phiaj xwm kho kom raug ntau dua rau cov neeg mob tuberculosis, nws yog ib qho tsim nyog yuav tsum kuaj xyuas cov tshuaj tiv thaiv kab mob, tshwj xeeb tshaj yog rifampicin tsis kam, uas tau dhau los ua cov kauj ruam kuaj pom zoo los ntawm WHO hauv kev kho mob tuberculosis.^[3]. Txawm hais tias qhov kev tshawb pom ntawm rifampicin tsis kam yog yuav luag sib npaug rau qhov kev tshawb pom ntawm MDR-TB, tsuas yog kuaj pom rifampicin tsis kam tsis quav ntsej cov neeg mob uas muaj mono-resistant INH (xa mus rau isoniazid tab sis rhiab heev rau rifampicin) thiab mono-resistant rifampicin (rhiab heev rau isoniazid tab sis tsis kam mus rau cov neeg mob), kev kho mob rifampicin. kev cai. Yog li ntawd, kev sim tshuaj tiv thaiv isoniazid thiab rifampicin yog qhov tsim nyog tsawg kawg nkaus hauv txhua qhov kev tswj hwm DR-TB.^[⁴^].

Technical Parameters

Cia	≤-18 ℃
Txee lub neej	12 hli
Hom qauv	Sputum Sample, Solid Culture (LJ Medium), Liquid Culture (MGIT Medium)
CV	<5.0%
LoD	Lub LoD ntawm cov khoom siv rau kev kuaj xyuas Mycobacterium tuberculosis yog 10 kab mob / mL;LoD ntawm cov khoom siv rau kev kuaj rifampicin hom tsiaj qus thiab hom mutant yog 150 kab mob / mL; LoD ntawm cov khoom siv rau kev tshawb nrhiav isoniazid tsiaj qus thiab mutant hom yog 200 kab mob / mL.
Qhov tshwj xeeb	1) Tsis muaj qhov cuam tshuam cuam tshuam thaum siv cov khoom siv los kuaj xyuas tib neeg genomic DNA (500ng), lwm yam 28 hom kab mob ua pa, thiab 29 hom tsis-tuberculous mycobacteria (raws li qhia hauv Table 3).2) Tsis muaj cov tshuaj tiv thaiv kab mob sib kis thaum siv cov khoom siv txhawm rau txheeb xyuas qhov chaw hloov pauv ntawm lwm cov tshuaj tiv thaiv kab mob ntawm rifampicin thiab isoniazid rhiab heev Mycobacterium tuberculosis (raws li qhia hauv Table 4).3) Cov tshuaj cuam tshuam ntau hauv cov qauv uas yuav tsum tau kuaj, xws li rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), namivinamide (45mg/L), dexamethasone (20mg / L) cov tshuaj, tsis muaj kev cuam tshuam rau cov txiaj ntsig ntawm cov khoom siv.
Cov cuab yeej siv tau	SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Real-Time PCR System