Mycobacterium Tuberculosis Nucleic Acid thiab Rifampicin (RIF), Kev Tiv Thaiv (INH)

Kev Piav Qhia Luv:

Cov khoom siv no yog siv rau kev kuaj pom Mycobacterium tuberculosis DNA hauv tib neeg cov hnoos qeev, cov khoom siv kho mob (LJ Medium) thiab cov kua kho mob (MGIT Medium), cov kua dej ntxuav ntsws, thiab cov kev hloov pauv hauv thaj tsam 507-533 amino acid codon (81bp, thaj tsam txiav txim siab qhov tsis kam ntawm rifampicin) ntawm rpoB gene ntawm Mycobacterium tuberculosis rifampicin, nrog rau cov kev hloov pauv hauv cov chaw hloov pauv tseem ceeb ntawm Mycobacterium tuberculosis isoniazid tsis kam. Nws muab kev pab rau kev kuaj mob Mycobacterium tuberculosis kab mob, thiab nws ntes cov noob tiv thaiv tseem ceeb ntawm rifampicin thiab isoniazid, uas pab nkag siab txog kev tiv thaiv tshuaj ntawm Mycobacterium tuberculosis uas tus neeg mob kis.

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HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid thiab Rifampicin (RIF), (INH) Kev Tshawb Fawb Khoom Siv (Kab Melting)

Kev Tshawb Fawb Txog Kab Mob Sib Kis

Mycobacterium tuberculosis, luv luv li Tubercle bacillus (TB), yog cov kab mob pathogenic uas ua rau mob ntsws, thiab tam sim no, cov tshuaj tiv thaiv kab mob ntsws thawj zaug uas siv feem ntau suav nrog isoniazid, rifampicin thiab ethambutol, thiab lwm yam.^[1]Txawm li cas los xij, vim yog siv tshuaj tiv thaiv kab mob ntsws tsis raug thiab cov yam ntxwv ntawm cov qauv phab ntsa ntawm mycobacterium tuberculosis nws tus kheej, mycobacterium tuberculosis tau tsim kev tiv thaiv tshuaj rau cov tshuaj tiv thaiv kab mob ntsws, thiab ib daim ntawv txaus ntshai tshwj xeeb yog multidrug-resistant tuberculosis (MDR-TB), uas tiv taus ob hom tshuaj feem ntau thiab zoo, rifampicin thiab isoniazid^[2].

Qhov teeb meem ntawm kev tiv thaiv tshuaj tiv thaiv kab mob ntsws muaj nyob rau txhua lub tebchaws uas WHO tau tshawb fawb. Yuav kom muab tau cov phiaj xwm kho mob kom raug dua rau cov neeg mob ntsws, nws yog ib qho tsim nyog los nrhiav kev tiv thaiv tshuaj tiv thaiv kab mob ntsws, tshwj xeeb tshaj yog kev tiv thaiv rifampicin, uas tau dhau los ua ib kauj ruam kuaj mob uas WHO pom zoo hauv kev kho mob ntsws.^[3]Txawm hais tias qhov kev tshawb pom ntawm kev tiv thaiv rifampicin yuav luag sib npaug rau qhov kev tshawb pom ntawm MDR-TB, tsuas yog kev kuaj pom kev tiv thaiv rifampicin tsis quav ntsej cov neeg mob uas muaj mono-resistant INH (hais txog kev tiv thaiv rau isoniazid tab sis rhiab heev rau rifampicin) thiab mono-resistant rifampicin (kev rhiab heev rau isoniazid tab sis tiv thaiv rifampicin), uas yuav ua rau cov neeg mob raug kev kho mob thawj zaug tsis tsim nyog. Yog li ntawd, kev kuaj mob tiv thaiv isoniazid thiab rifampicin yog qhov yuav tsum tau ua tsawg kawg nkaus hauv txhua qhov kev pab cuam tswj DR-TB.^[⁴^].

Cov Kev Ntsuas Kev Txawj

Kev Khaws Cia	≤-18 ℃
Lub sijhawm khaws cia	12 lub hlis
Hom Qauv	Qauv hnoos qeev, Kev kuaj kab mob khov kho (LJ Medium), Kev kuaj kab mob ua kua (MGIT Medium)
CV	<5.0%
LoD	LoD ntawm cov khoom siv rau kev kuaj mob Mycobacterium tuberculosis yog 10 kab mob / mL;LoD ntawm cov khoom siv rau kev kuaj pom rifampicin hom qus thiab hom mutant yog 150 kab mob / mL; LoD ntawm cov khoom siv rau kev kuaj pom isoniazid hom qus thiab hom mutant yog 200 kab mob / mL.
Kev Tshwj Xeeb	1) Tsis muaj kev sib tshuam thaum siv cov khoom siv los nrhiav cov DNA genomic tib neeg (500ng), lwm 28 hom kab mob ua pa, thiab 29 hom kab mob mycobacteria uas tsis yog tuberculous (raws li qhia hauv Rooj 3).2) Tsis muaj kev sib tshuam thaum siv cov khoom siv los ntes cov chaw hloov pauv ntawm lwm cov noob caj noob ces tiv taus tshuaj ntawm rifampicin thiab isoniazid rhiab heev Mycobacterium tuberculosis (raws li qhia hauv Rooj 4).3) Cov tshuaj uas cuam tshuam rau hauv cov qauv kuaj uas yuav kuaj, xws li rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L), tsis muaj feem cuam tshuam rau cov txiaj ntsig ntawm cov khoom siv kuaj.
Cov Cuab Yeej Siv Tau	SLAN-96P Cov Txheej Txheem PCR Real-Time (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm