Mycobacacterium tuberculosis nucleic acid thiab rifampicin tsis kam
Khoom npe
Hwts-rt074b-mycobacterium tuberculosis nucleic acid thiab rifampicin tsis mus nrhiav khoom siv (Melting nkhaus)
Daim ntawv muaj cai
CE
Kev kawm
Mycobacacterium tuberculosis, tsis ntev li noogercle Bacillus, TB, yog tus kab mob kab mob uas ua rau muaj kev mob ntsws. Tam sim no, feem ntau siv thawj-kab anti-tuberculosis tshuaj suav nrog fluoroquinolones yog kab ntawv tshiab, buildani thiab delamani, thiab lwm yam Cov. Txawm li cas los xij, vim qhov tsis raug ntawm kev siv Anti-Toberculosis cov tshuaj thiab cov yam ntxwv ntawm lub tsev phab ntsa qauv ntawm mycobacacterium tuberculosis, myCobacacterium tuberculosis, uas yog cov kev nyuaj rau kev tiv thaiv thiab kev kho mob ntsws.
Rifampicin tau siv dav hauv kev kho mob ntawm cov neeg mob ntsws tuberculosis thaum xyoo 1970, thiab muaj cov nyhuv tseem ceeb. Nws tau xaiv thawj zaug kom luv luv ntawm kev siv tshuaj kho mob ntawm cov neeg mob ntsws qhuav tuberculosis. Rifampicin tsis yog feem ntau tshwm sim los ntawm kev hloov pauv ntawm RPOB RPE. Txawm tias cov tshuaj tiv thaiv kab mob tuberculosis tshiab yog qhov tsis txaus ntseeg, tseem muaj cov neeg mob tsis tu ncua, thiab qhov tshwm sim tsis zoo ntawm kev ua haujlwm ntawm cov tshuaj tua kab mob ntawm cov tshuaj tua kab mob tsis zoo. Pom tseeb, cov mycobacterium tuberculosis hauv cov neeg mob ntsws ntsws tsis muaj kev phom sij, thiab ua kom muaj kev pheej hmoo tuag ntawm tus neeg mob.
Tshooj
| Tshooj | Raws thiab fluorophores | Cov tshuaj tiv thaiv tsis a | Cov tshuaj tiv thaiv tsis b | Cov tshuaj tiv thaiv buffer c |
| Fam Channel | Reporter: KAM, QENYER: Tsis muaj | RPOB 507-514 | RPOB 513-520 | 38KD thiab IS6110 |
| CY5 Channel | Reporter: cy5, quencher: tsis muaj | RPOB 520-527 | RPOB 527-533 | / |
| Hex (Vic) Channel | Reporter: Hex (Vic), Quencher: Tsis muaj | Sab hauv tswj | Sab hauv tswj | Sab hauv tswj |
Kev tsis muaj
| Chaw ntim khoom | ≤-18 ℃ hauv kev tsaus ntuj |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | Nto |
| CV | APR5.0% |
| Lub lab | MyCobacterium Tuberculosis 50 Cov Kab Mob / ML Rifampicin-resistant qus hom: 2x103Cov kab mob / ML Homozygous Mutant: 2x103Cov kab mob / ML |
| Yam tshwj xeeb | Nws kuaj tau tsiaj qus-hom mycobacterium tuberculosis thiab cov kev sib hloov pauv cov txiaj ntsig zoo li cov rifampicin, uas txhais tau tias tsis muaj cross-reactivity. |
| Muaj cov cuab yeej siv: | Slan-96p ROOST-SICTICE PRRY Biorad Cfx96 REO-SIJHAWM PCR system Lightcycler480 ®-Time Pricr System |
Ua Haujlwm Ntws
Yog tias siv lub macro & micro-kev sim DNA / RNA cov khoom (HWTS-3019-48, uas tuaj yeem siv nrog Macro & Micro-xeem tsis siv neeg Nucleic Acid Extraction (HWTS-3006C, HWTS-3006B)) lossis Macro & Micro-Kev Ntsuas Kab Mob DNA / RNA Kem (hwts-3022-50) los ntawm Jiangsu Macro & Micregs thiab 200μl ntawm kev tswj hwm tau zoo, thiab ntxiv 10μl ntawm lub Sab hauv kev tswj cais mus rau hauv kev tswj tau zoo, kev tswj hwm tsis zoo thiab ua tiav hnoos qeev kom raug kuaj, thiab cov kauj ruam tom ntej yuav tsum tau nruj me ntsis raws li cov lus qhia. Cov qauv rho tawm ntim yog 200μL, thiab cov lus pom zoo kom pom zoo meej yog 100μL.
