Mycoplasma Pneumoniae (MP)
Khoom npe
HWTS-RT024 Mycoplasma Pneumoniae (MP) Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Mycoplasma pneumoniae (MP) yog ib hom kab mob prokaryotic tsawg tshaj plaws, uas nyob nruab nrab ntawm cov kab mob thiab cov kab mob, nrog cov qauv ntawm tes tab sis tsis muaj phab ntsa ntawm tes.MP feem ntau ua rau tib neeg muaj kab mob ua pa, tshwj xeeb tshaj yog rau cov menyuam yaus thiab cov hluas.Nws tuaj yeem ua rau tib neeg mycoplasma pneumonia, menyuam yaus txoj kev ua pa thiab atypical pneumonia.Cov kev kho mob tshwm sim muaj ntau yam, feem ntau yog hnoos hnyav, ua npaws, ua daus no, mob taub hau, mob caj pas.Cov kab mob ua pa sab saud thiab mob ntsws bronchial yog qhov tshwm sim ntau tshaj.Qee tus neeg mob tuaj yeem txhim kho los ntawm kab mob ua pa sab sauv mus rau mob ntsws loj, mob ua pa hnyav thiab tuag taus.
Channel
FAM | Mycoplasma pneumoniae |
VIC/HEX | Internal Control |
Technical Parameters
Cia | ≤-18 ℃ |
Txee lub neej | 12 hli |
Hom Qauv | Sputum, Oropharyngeal swab |
Ct | ≤38 |
CV | ≤ 5.0% |
LoD | 200 daim / ml |
Qhov tshwj xeeb | a) Hla reactivity: tsis muaj cross reactivity nrog Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus pneumoniae, s aeruginosa, Acinetobacter baumannii, Influenza A virus , Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus and human genomic nucleic acid. b) Kev muaj peev xwm tiv thaiv kev cuam tshuam: tsis muaj kev cuam tshuam thaum cov tshuaj cuam tshuam tau sim nrog cov ntsiab lus hauv qab no: hemoglobin (50mg / L), bilirubin (20mg / dL), mucin (60mg / mL), 10% (v / v) tib neeg cov ntshav, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg. /L), minocycline (0.1g / L). |
Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR System Siv Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer thev naus laus zis) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
(1) Sputum Sample
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Tsis Siv Neeg Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ntxiv 200µL ntawm ib txwm saline rau cov txheej txheem precipitate.Kev rho tawm tom qab yuav tsum tau ua raws li cov lus qhia rau kev siv.Qhov pom zoo elution ntim yog 80µL.Recommended extraction reagent: Nucleic Acid Extraction los yog Purification Reagent (YDP315-R).Kev rho tawm yuav tsum tau ua nruj me ntsis raws li cov lus qhia rau kev siv.Qhov pom zoo elution ntim yog 60µL.
(2) Oropharyngeal swab
Pom zoo extraction reagent: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Tsis Siv Neeg Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.Qhov pom zoo extraction ntim ntawm cov qauv yog 200µL, thiab qhov pom zoo elution ntim yog 80µL.Recommended extraction reagent: QIAamp Viral RNA Mini Kit (52904) los yog Nucleic Acid Extraction los yog Purification Reagent (YDP315-R).Kev rho tawm yuav tsum tau ua nruj me ntsis raws li cov lus qhia rau kev siv.Qhov pom zoo rho tawm ntim ntawm cov qauv yog 140µL, thiab qhov pom zoo elution ntim yog 60µL.