Pawg B Streptococcus nucleic acid kuaj cov khoom siv (Enzymatic Probe Isothermal Amplification)
1.Kev txheeb xyuas qhov tseem ceeb
Pab pawg B streptococcus (GBS) feem ntau yog nyob rau hauv cov poj niam qhov chaw mos thiab lub qhov quav, uas tuaj yeem ua rau muaj kab mob ntxov ntxov (GBS-EOS) hauv cov menyuam mos los ntawm txoj kab ntsug ntawm niam mus rau menyuam, thiab yog qhov ua rau muaj mob ntsws neonatal, meningitis, septicemia thiab txawm tuag.Xyoo 2021, Cov Kws Tshaj Lij Pom Zoo rau Kev Tswj Xyuas Kev Kho Mob ntawm Cov Menyuam mos uas muaj kev pheej hmoo siab nrog kev mob ntxov ntxov nyob rau hauv tib chav ntawm Cov Niam thiab Cov Me Nyuam ntawm Tuam Tshoj Lub Koom Haum Saib Xyuas Kev Noj Qab Haus Huv thiab Menyuam Yaus tau qhia tias kev tshuaj xyuas GBS 35-37 lub lis piam ua ntej yug menyuam thiab kev tiv thaiv kab mob hauv lub cev (IAP ) yog cov kev ntsuas zoo los tiv thaiv GBS-EOS hauv cov menyuam mos.
2. Cov teeb meem ntsib los ntawm txoj kev tshawb pom tam sim no
Premature rupture of membranes (PROM) yog hais txog qhov rupture ntawm daim nyias nyias ua ntej ua hauj lwm, uas yog ib qho teeb meem tshwm sim nyob rau hauv perinatal lub sij hawm.Kev puas tsuaj ntxov ntxov ntawm daim nyias nyias Vim yog qhov tawg ntawm daim nyias nyias, GBS nyob rau hauv qhov chaw mos ntawm cov poj niam tsis muaj hnub nyoog yuav kis tau mus rau sab saud, ua rau muaj kab mob hauv lub cev.Qhov kev pheej hmoo ntawm kev kis kab mob yog ncaj qha rau kev loj hlob thaum lub sijhawm tawg ntawm daim nyias nyias (> 50% ntawm cov poj niam cev xeeb tub yug hauv 1-2 teev tom qab tawg ntawm daim nyias nyias, lossis txawm tias 1-2 teev).
Cov txheej txheem tshawb nrhiav uas twb muaj lawm tsis tuaj yeem ua tau raws li qhov yuav tsum tau ua raws sijhawm (< 1h), qhov tseeb thiab kev hu rau GBS kuaj pom thaum lub sijhawm xa khoom.
| Kev ntsuas ntsuas | Kab lis kev cai | Culture lub sij hawm: 18-24hYog tias qhov ntsuas tshuaj rhiab heev: nce 8-16h | 60% kev kuaj pom zoo;Thaum lub sij hawm kuaj cov txheej txheem, nws raug rau cov kab mob xws li Enterococcus faecalis nyob ib ncig ntawm qhov chaw mos thiab qhov quav, ua rau cov txiaj ntsig tsis zoo / cuav. |
| Immunochromatography | Lub sijhawm kuaj: 15 min. | Lub rhiab heev yog tsawg, thiab nws yog ib qho yooj yim kom tsis pom kev, tshwj xeeb tshaj yog thaum tus nqi ntawm cov kab mob me me, nws yog ib qho nyuaj rau kev soj ntsuam, thiab cov lus qhia tsis pom zoo. | |
| PCR | Lub sijhawm kuaj: 2-3h | Lub sijhawm kuaj pom yog ntau tshaj 2 teev, thiab PCR ntsuas yuav tsum tau kuaj hauv cov khoom siv, thiab nws tsis tuaj yeem ua raws li qhov ntsuas no. |
3. Macro & Micro-Test cov khoom tseem ceeb
Kev tshawb pom sai: Siv cov patented enzyme digestion sojntsuam qhov kub thiab txias amplification txoj kev, cov neeg mob zoo tuaj yeem paub qhov tshwm sim sai li 5 feeb.
Kev kuaj xyuas txhua lub sijhawm, tsis tas yuav tos: nws tau nruab nrog qhov ntsuas kub tsis tu ncua nucleic acid amplification analyzer Yooj yim Amp, thiab plaub lub modules khiav ntawm nws tus kheej, thiab cov qauv raug tshuaj xyuas thaum lawv tuaj txog, yog li tsis tas yuav tos cov qauv.
Ntau hom qauv: qhov chaw mos swab, qhov quav qhov quav lossis qhov chaw mos sib xyaw tuaj yeem kuaj pom, uas ua tau raws li cov lus pom zoo ntawm GBS cov lus qhia, txhim kho qhov kev kuaj pom zoo thiab txo qis tus nqi misdiagnosis.
Kev ua tau zoo heev: ntau qhov chaw loj cov qauv kuaj xyuas (> 1000 tus neeg mob), rhiab heev 100%, qhov tshwj xeeb 100%.
Qhib reagent: tau tshaj tam sim no lub ntsiab fluorescence quantitative PCR ntsuas.
4. Cov ntaub ntawv khoom
| Yam khoom naj npawb | Khoom npe | Specification | Daim ntawv pov thawj tus lej |
| HWM-UR033C | Pawg B Streptococcus nucleic acid nrhiav cov khoom siv (Enzymatic sojntsuam Isothermal Amplification) | 50 test/kit | Tuam Tshoj Machinery Sau Npe 20243400248 |
| HWM-EQ008 | Yooj Yim Amp Real-time Fluorescence Isothermal Detection System | HWTS-1600P (4-channel) | Tuam Tshoj Machinery Sau Npe 20233222059 |
| HWTS-1600S (2-channel) | |||
| HWM-EQ009 |
Post lub sij hawm: Mar-07-2024