—— Kev Kawm Ua Ke los ntawm Zhejiang CDC, Macro & Micro-Test, thiab Tuam Tshoj CDC Luam tawm hauv Frontiers hauv Cellular thiab Infection Microbiology
Kev Txheeb Xyuas Txog Kev Kawm
Thaum lub Tsib Hlis 2026, Frontiers in Cellular and Infection Microbiology (JCR Q1, IF ≈ 4.6) tau luam tawm ib daim ntawv uas coj los ntawm Zhejiang Provincial Center for Disease Control and Prevention (Zhejiang CDC), nrog rau pab pawg bioinformatics los ntawm Beijing Macro & Micro-Test Bio-Tech Co., Ltd. thiab National Institute for Communicable Disease Control and Prevention (Tuam Tshoj CDC) ua cov kws sau ntawv. Txoj kev tshawb fawb no muaj npe hu ua:
"Kev txheeb xyuas thiab kev tshuaj xyuas phylogenetic ntawm xya hom kab mob Brucella abortus hauv Zhejiang, Suav teb."
Txoj kev tshawb fawb no sawv cev rau thawj qhov kev tshuaj xyuas kab mob Brucella abortus (B. abortus) uas siv cov txheej txheem genome los nrhiav kab mob hauv Zhejiang xeev, Suav teb. Pab neeg tau tshuaj xyuas xya hom kab mob uas tau sau los ntawm xyoo 2015 txog 2025 (plaub hom kab mob uas yog los ntawm tib neeg thiab peb hom kab mob uas yog los ntawm nyuj los ntawm Jinhua, Quzhou, thiab Ningbo). Cov kev tshawb pom no muab cov pov thawj genomic rau txoj kev pib thiab txoj kev sib kis ntawm "hom kab mob uas muaj zog nyob rau sab qaum teb" no hauv thaj chaw muaj kab mob sib kis tsis zoo li qub nyob rau sab qab teb sab hnub tuaj Suav teb.
Keeb Kwm thiab Qhov Tseem Ceeb
Kab mob Brucellosis yog ib yam kab mob uas kis tau los ntawm cov kab mob Brucella. Kab mob Brucella abortus feem ntau kis rau nyuj tab sis kuj tuaj yeem ua rau tib neeg muaj kab mob. Hauv Suav teb, kab mob brucellosis qhia txog kev hloov pauv ntawm thaj chaw: qhov tshwm sim ntau tshaj plaws tshwm sim hauv cov xeev sab qaum teb (piv txwv li, Inner Mongolia, Shanxi, Heilongjiang). Los ntawm qhov sib piv, cov xeev sab qab teb, suav nrog Zhejiang, keeb kwm tau muaj kab mob Brucella melitensis ntau tshaj plaws, nrog rau ob peb tus neeg mob B. abortus uas tau tshaj tawm. Qhov sib txawv ntawm thaj chaw no ua rau kev txheeb xyuas cov noob caj noob ces thiab qhov chaw nrhiav B. abortus hauv Zhejiang yog qhov tseem ceeb rau kev noj qab haus huv pej xeem.
Cov Txheej Txheem thiab Cov Ntsiab Lus Tseem Ceeb
Cov pab pawg tshawb fawb tau siv ntau txoj kev npaj sib xyaw ua ke ntawm molecular biology thiab bioinformatics:
1.Kev txheeb xyuas tus kab mob thiab kev ntaus ntawv yooj yim
BCSP-31 gene PCR thiab AMOS-PCR lees paub tias tag nrho xya tus isolates yog B. abortus.
Kev ntsuas kab ke ntawm ntau qhov chaw (MLST) raws li cuaj lub noob caj noob ces hauv tsev qhia tau tias txhua qhov isolates koom nrog hom kab ke ST2, qhia txog kev sib xws ntawm cov noob caj noob ces ntawm B. abortus hauv Zhejiang.
2.Kev piav qhia txog tag nrho cov genome
Kev ua qhov kev txheeb xyuas tag nrho ntawm cov noob caj noob ces tau ua tiav ntawm Illumina NovaSeq platform. Kev tshuaj xyuas qhov nruab nrab ntawm nucleotide identity (ANI) qhia tau tias cov Zhejiang isolates sib koom txog li 99.99% zoo sib xws nrog cov qauv siv B. abortus 544.
Kev tshuaj xyuas pan-genome qhia tau hais tias muaj cov pej xeem uas tseem khaws cia zoo heev: 3,084 cov noob caj noob ces tseem ceeb tau txheeb xyuas, nrog rau tsuas yog 10 cov noob caj noob ces ntawm lub plhaub xwb, thiab tsis pom cov noob caj noob ces mos lossis cov noob caj noob ces huab.
3.Cov qauv ntawm cov noob caj noob ces virulence thiab antimicrobial tsis kam
Tag nrho muaj 68 yam cuam tshuam txog kev ua phem tau kwv yees, uas suav nrog cov txheej txheem ib txwm muaj xws li LPS biosynthesis, T4SS secretion system, thiab BvrR-BvrS ob-component regulatory system. Qhov tseem ceeb, txhua qhov isolates tsis muaj cov adhesin genes bmaA thiab btaF. Kev tshuaj xyuas cov noob tiv thaiv tsuas yog pom cov noob mprF hauv CARD database, tsis muaj lwm yam kev txiav txim siab tiv thaiv tau txheeb xyuas.
4. Kev tsim kho dua tshiab ntawm phylogenetic thiab kev sib kis kab mob
Kev tshuaj xyuas polymorphism ntawm cov noob caj noob ces (core-genome single-nucleotide polymorphism - cgSNP) tau muab cov noob caj noob ces Zhejiang cais tawm rau ntawm ib qho chaw tshwj xeeb hauv tsob ntoo phylogenetic thoob ntiaj teb. Cov txiaj ntsig tau qhia tias cov noob caj noob ces Zhejiang tsim ib pawg monophyletic ua ke nrog cov noob caj noob ces los ntawm Russia, Mongolia, thiab ntau lub xeev sab qaum teb Suav (Ningxia, Heilongjiang, Inner Mongolia, Hebei, Gansu, Beijing). Pawg no faib ua peb pawg me sib txawv (Clade 1–3), qhia txog ntau qhov xwm txheej kev qhia tawm ywj pheej.
Cov Lus Xaus thiab Cov Kev Cuam Tshuam
Daim ntawv tshawb fawb no muab thawj cov ntaub ntawv genomic tseeb siab ntawm B. abortus hauv Zhejiang xeev thiab muab ntau qhov xaus lus tseem ceeb:
- Clear keeb kwm yav dhau los ntawm noob caj noob ces– Cov kab mob B. abortus uas kis thoob plaws hauv Zhejiang yog cov kab mob ST2, muaj cov genomic zoo heev, thiab sawv cev rau cov kab mob brucellosis bovine brucellosis ib txwm muaj.
2. Evikev sib kis thoob plaws cheeb tsam- Kev tshuaj xyuas txog phylogenetic tsis txhawb nqa qhov muaj ib tsev neeg nyob hauv Zhejiang. Hloov chaw, cov ntaub ntawv qhia meej tias cov kab mob no tau pib los ntawm sab qaum teb Suav teb thiab tej zaum yuav muaj keeb kwm yav dhau los zoo ib yam li cov kab mob los ntawm Russia thiab Mongolia. Qhov muaj peb pawg me me txhais tau tias muaj ntau qhov xwm txheej sib txawv.
3. Cov teeb meem kev noj qab haus huv pej xeem– Cov kev tshawb pom qhia txog qhov tseem ceeb ntawm kev soj ntsuam genomic rau brucellosis txawm tias nyob rau hauv thaj chaw ib txwm tsis muaj kab mob xws li Zhejiang. Txawm hais tias tus lej tam sim no tsawg, cov cuab yeej daws teeb meem siab xws li cgSNP tuaj yeem taug qab qhov chaw ntawm kev sib kis kab mob los ntawm txawv teb chaws thiab muab pov thawj kev tshawb fawb los cuam tshuam cov saw hlau sib kis uas cuam tshuam nrog kev thauj tsiaj txhu mus rau lwm lub xeev.
Txoj haujlwm no tsis yog tsuas yog ua kom tiav qhov kev tshawb fawb tsis txaus hauv xeev Zhejiang xwb tab sis kuj muab cov ntaub ntawv tshiab rau kev saib xyuas cov kab mob thiab kev ntsuam xyuas kev pheej hmoo ntawm tus kab mob brucellosis hauv thaj tsam Yangtze River Delta.
Cov Ntaub Ntawv Ntawv:
Yang, Y., Shi, X., Chen, J., Wang, L., Wu, Z., Yao, W., … & Wu, B. (2026). Kev txheeb xyuas thiab kev tshuaj xyuas phylogenetic ntawm xya hom kab mob Brucella abortus hauv Zhejiang, Suav teb. Frontiers hauv Cellular thiab Infection Microbiology, 16, 1758965.
Lub sijhawm tshaj tawm: Lub Rau Hli-10-2026