14 Hom Neeg Papillomavirus uas pheej hmoo siab (16/18/52 Typing)
Khoom npe
HWM-CC019A-14 Hom Neeg Papillomavirus uas pheej hmoo siab (16/18/52 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Kab mob sib kis
Cov kev tshawb fawb tau pom tias muaj kab mob HPV tsis tu ncua thiab ntau yam kab mob yog ib qho ntawm cov ua rau mob qog noj ntshav.Tam sim no, cov kev kho mob uas tau lees paub tseem tsis muaj rau kev mob qog noj ntshav hauv tsev menyuam los ntawm HPV, yog li kev tshawb pom ntxov thiab kev tiv thaiv kab mob hauv tsev menyuam los ntawm HPV yog tus yuam sij rau kev tiv thaiv kab mob qog noj ntshav.Nws yog ib qho tseem ceeb heev los tsim ib qho yooj yim, tshwj xeeb thiab ceev etiology diagnostic test rau kev kuaj mob thiab kev kho mob ntawm ncauj tsev menyuam.
Channel
| Channel | Hom |
| FAM | HPV 18 |
| VIC/HEX | HPV 16 |
| ROX | HPV 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 |
| CY 5 | HPV 52 |
| qis 705/CY5.5 | Internal Control |
Technical Parameters
| Cia | ≤-18 ℃ |
| Txee lub neej | 12 hli |
| Hom Qauv | Tso zis, Cervical Swab, Vaginal Swab |
| Ct | ≤ 28 |
| LoD | 300 daim / ml |
| Qhov tshwj xeeb | Tsis muaj kev sib cuam tshuam nrog lwm cov qauv ua pa xws li Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie, virus/Aptovirus, virus 2/A. B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. thiab human genomic DNA. |
| Cov cuab yeej siv tau | MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System thiab BioRad CFX Opus 96 Real-Time PCR System |
Ua hauj lwm Flow
1.Cov zis tso zis
A: Coj1.4mL ntawm cov zis coj mus kuaj thiab centrifuge ntawm 12000rpm rau 5 feeb;muab pov tseg lub supernatant (nws raug pom zoo kom khaws 10-20μL supernatant los ntawm hauv qab ntawm lub centrifuge raj), ntxiv 200μL ntawm cov qauv tso tawm reagent, thiab tom qab rho tawm yuav tsum tau ua raws li cov lus qhia rau kev siv Macro & Micro-Test Sample Tso Reagent (HWTS-3005-8).
B: Coj1.4mL ntawm cov zis coj mus kuaj thiab centrifuge ntawm 12,000rpm rau 5 feeb;pov tseg cov supernatant (nws pom zoo kom khaws 10-20μL ntawm supernatant los ntawm hauv qab ntawm lub centrifuge raj), thiab ntxiv 200μL ntawm ib txwm saline rau resuspend, raws li cov qauv yuav tsum tau kuaj.Kev rho tawm tom ntej tuaj yeem ua nrog Macro & Micro-Test Viral DNA / RNA Cov Khoom Siv (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.s rau siv.Qhov pom zoo elution ntim yog 80μL.
C: Noj1.4mL ntawm cov zis coj mus kuaj thiab centrifuge ntawm 12,000rpm rau 5 feeb;pov tseg cov supernatant (nws pom zoo kom khaws 10-20μL ntawm supernatant los ntawm hauv qab ntawm lub centrifuge raj), thiab ntxiv 200μL ntawm ib txwm saline rau resuspend, raws li cov qauv yuav tsum tau kuaj.Cov extraction tom ntej tuaj yeem ua nrogQIAamp DNA Mini Kit (51304) los ntawm QIAGEN lossis Macro & Micro-Test Viral DNA/RNA Kem (HWTS-3020-50).Kev rho tawm yuav tsum tau ua tiav raws li cov lus qhia rau kev siv.Qhov ntim ntawm lub extraction yog 200μL, thiab qhov pom zoo elution ntim yog 80μL.
2. Cervical swab/vaginal swab sample
A: Siv 1mL ntawm cov qauv mus kuaj hauv 1.5mLof centrifuge tube,thiabcentrifuge ntawm 12000rpm rau 5 feeb. Discard lub supernatant (nws raug nquahu kom khaws 10-20μL ntawm supernatant los ntawm hauv qab ntawm lub centrifuge raj), ntxiv 100μL ntawm cov qauv tso tawm reagent, thiab ces rho tawm raws li cov lus qhia rau kev siv Macro & Micro-Test Sample Reagent ( HWM-3005-8).
B: Kev rho tawm tuaj yeem ua nrog Macro & Micro-Test Viral DNA / RNA Cov Khoom Siv (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (uas tuaj yeem siv nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. nruj raws li cov lus qhia siv.Cov qauv muab rho tawm yog 200μL, thiab qhov pom zoo elution ntim yog 80μL.
C: Kev rho tawm tuaj yeem ua nrog QIAamp DNA Mini Cov Khoom Siv (51304) los ntawm QIAGEN lossis Macro & Micro-Test Viral DNA / RNA Kem (HWTS-3020-50).Kev rho tawm yuav tsum tau ua tiav raws li cov lus qhia rau kev siv.Cov qauv rho tawm yog 200 μL, thiab qhov pom zoo elution ntim yog80l miv.
3. Cervical Swab/ Vaginal Swab
Ua ntej sampling, siv cov paj rwb swab maj mam so cov khoom tso tawm ntawm lub ncauj tsev menyuam, thiab siv lwm cov paj rwb swab infiltrated nrog lub cell preservation tov los yog lub ncauj tsev menyuam exfoliated cell sampling txhuam kom cling rau lub ncauj tsev menyuam mucosa thiab tig clockwise 3-5 rounds kom tau. cervical exfoliated cell.Maj mam tshem tawm cov paj rwb swab los yog txhuam,thiabmuab tso rau hauv ib lub raj coj mus kuaj nrog 1mL ntawm sterile ib txwm saline. ATom qab yaug tag nrho, nyem cov paj rwb qhuav los yog txhuam rau ntawm phab ntsa ntawm lub raj thiab muab pov tseg, kaw lub raj kom nruj, thiab kos cov qauv npe (lossis tus lej) thiab ntaus rau ntawm lub raj xa dej.
