Cov Khoom Siv Kuaj Xyuas Kab Mob Dengue Virus I/II/III/IV Nucleic Acid Uas Khov Qhuav (Fluorescence PCR)-RUO
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Cov Khoom Siv Kuaj Xyuas Kab Mob Dengue Virus I/II/III/IV Nucleic Acid Uas Khov Qhuav (Fluorescence PCR)-RUO
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Kab mob Dengue fever (DF), uas yog los ntawm kev kis tus kab mob denguevirus (DENV), yog ib qho ntawm cov kab mob sib kis ntau tshaj plaws ntawm cov kab mob arbovirus. Nws cov kab mob sib kis suav nrog Aedes aegypti thiab Aedes albopictus. DF feem ntau pom muaj nyob rau hauv thaj chaw sov thiab thaj chaw sov. DENV yog flavivirus hauv flaviviridae, thiab tuaj yeem muab faib ua 4 hom kab mob raws li antigen ntawm qhov chaw. Cov tsos mob ntawm kev kis tus kab mob DENV feem ntau suav nrog mob taub hau, kub taub hau, qaug zog, cov qog ntshav loj hlob, leukopenia thiab lwm yam, thiab los ntshav, poob siab, raug mob siab lossis tuag hauv cov xwm txheej hnyav. Nyob rau xyoo tas los no, kev hloov pauv huab cua, kev loj hlob hauv nroog, kev txhim kho sai ntawm kev ncig tebchaws thiab lwm yam tau muab cov xwm txheej ceev thiab yooj yim dua rau kev kis thiab kis tus kab mob DF, ua rau thaj chaw sib kis ntawm DF nthuav dav tas li. Yog li ntawd, kev tsim kom muaj txoj kev kuaj mob yooj yim, tshwj xeeb thiab ceev ceev ntawm DENV muaj qhov tseem ceeb rau kev kuaj mob DF.
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | 2-8 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | serum |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Daim Ntawv Luam / mL |
| Kev Tshwj Xeeb | Cov kev kuaj tsis pom zoo rau cov qauv ntshav uas tsis muaj tus kab mob dengue; cov txiaj ntsig ntawm kev xeem cuam tshuam qhia tau tias thaum cov bilirubin concentration hauv cov ntshav tsis ntau tshaj 168.2μmol / mL, qhov concentration ntawm hemoglobin tsim los ntawm hemolysis tsis ntau tshaj 130g / L, cov ntshav lipid concentration tsis ntau tshaj 65mmol / mL, thiab tag nrho IgG concentration hauv cov ntshav tsis ntau tshaj 5mg / mL, tsis muaj kev cuam tshuam rau kev kuaj pom tus kab mob dengue; siv cov kab mob Hepatitis A, Hepatitis B virus, Hepatitis C virus, Herpes virus, Eastern equine encephalitis virus, Hantaan virus, Bunia virus, West Nile virus, Zika virus thiab cov qauv ntshav genomic tib neeg rau kev xeem hla-reactivity, thiab cov txiaj ntsig qhia tau tias tsis muaj kev cuam tshuam hla ntawm cov khoom siv no thiab cov kab mob saum toj no. |
| Cov Cuab Yeej Siv Tau | Hom I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Hom II: EudemonTMAIO800 (HWTS-EQ007) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Kev Ua Haujlwm
Cov tshuaj reagents xav tau tab sis tsis tau muab:
Hom I: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (uas siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Hom II: Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), siv tau nrog EudemonTM AIO800 (HWTS-EQ007) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ua ntej siv cov tshuaj reagent xeem hom II, tus neeg siv khoom muaj cov tshuaj reagent rho tawm thiab cov cuab yeej amplification txhawb nqa, thiab tus engineer yuav coj tus neeg siv khoom ua qhov kev sim thaum siv thawj zaug.
Cov khoom siv yuav tsum tau muab tab sis tsis tau muab:Cov Lus Qhia Tsis Muaj DNase/RNase, cov hnab looj tes pov tseg, cov raj DNase/RNase EP Tsis Muaj, centrifuge, lub rooj sawv hlau nplaum, 8-lub raj strips rau PCR.
