Cov Khoom Siv Tshawb Nrhiav Kev Hloov Pauv ntawm Tib Neeg EGFR Gene 29 (Fluorescence PCR)-RUO
Lub npe khoom
Cov Khoom Siv Tshawb Nrhiav Kev Hloov Pauv ntawm Tib Neeg EGFR Gene 29 (Fluorescence PCR)-RUO
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Mob qog noj ntshav hauv lub ntsws tau dhau los ua qhov ua rau muaj kev tuag ntau tshaj plaws thoob ntiaj teb, ua rau tib neeg muaj kev phom sij heev rau kev noj qab haus huv. Mob qog noj ntshav hauv lub ntsws uas tsis yog me me muaj li ntawm 80% -85% ntawm cov neeg mob qog noj ntshav hauv lub ntsws. EGFR tam sim no yog lub hom phiaj tseem ceeb tshaj plaws rau kev kho mob qog noj ntshav hauv lub ntsws uas tsis yog me me. Kev phosphorylation ntawm EGFR tuaj yeem txhawb kev loj hlob ntawm cov hlwb qog, kev sib txawv, kev nkag mus, metastasis, tiv thaiv apoptosis, thiab txhawb kev loj hlob ntawm cov hlwb qog. EGFR tyrosine kinase inhibitors (TKI) tuaj yeem thaiv txoj kev teeb tsa EGFR los ntawm kev txwv tsis pub EGFR autophosphorylation, yog li ntawd txwv tsis pub kev loj hlob thiab kev sib txawv ntawm cov hlwb qog, txhawb kev loj hlob ntawm cov hlwb qog, txo cov hlwb qog, thiab lwm yam, kom ua tiav kev kho mob rau cov hlwb qog [1]. Muaj ntau txoj kev tshawb fawb tau qhia tias kev kho mob ntawm EGFR-TKI muaj feem cuam tshuam nrog qhov xwm txheej ntawm EGFR gene mutation, thiab tuaj yeem tshwj xeeb txwv tsis pub kev loj hlob ntawm cov hlwb qog nrog EGFR gene mutation [2,3]. Tus noob caj noob ces EGFR nyob rau ntawm caj npab luv ntawm chromosome 7 (7p12), nrog rau tag nrho ntev ntawm 200Kb thiab muaj 28 exons. Thaj chaw hloov pauv feem ntau nyob hauv exons 18 txog 21, codons 746 txog 753 deletion mutation ntawm exon 19 suav txog li 45% thiab L858R mutation ntawm exon 21 suav txog li 40% txog 45% [4]. Cov Lus Qhia ntawm NCCN rau Kev Kuaj Mob thiab Kev Kho Mob Cancer Ntsws Tsis Me Me hais meej meej tias kev kuaj mob hloov pauv noob caj noob ces EGFR yog qhov yuav tsum tau ua ntej kev tswj hwm EGFR-TKI. Cov khoom siv kuaj mob no yog siv los coj kev tswj hwm ntawm cov tshuaj epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), thiab muab lub hauv paus rau kev kho mob tus kheej rau cov neeg mob uas muaj mob qog nqaij hlav tsis me me. Cov khoom siv no tsuas yog siv rau kev kuaj pom cov kev hloov pauv feem ntau hauv EGFR gene hauv cov neeg mob uas muaj mob qog nqaij hlav tsis me me. Cov txiaj ntsig ntawm kev xeem tsuas yog rau kev siv hauv kev kho mob xwb thiab yuav tsum tsis txhob siv ua lub hauv paus rau kev kho mob rau tus kheej ntawm cov neeg mob. Cov kws kho mob yuav tsum xav txog tus neeg mob tus mob, cov cim qhia txog tshuaj, thiab kev kho mob. Cov tshuaj tiv thaiv thiab lwm yam cim qhia kev kuaj hauv chaw kuaj mob thiab lwm yam yog siv los txiav txim siab txog cov txiaj ntsig ntawm kev xeem.
Channel
| Cov tshuaj tiv thaiv tsis haum | Channel |
| EGFR IC Reaction Buffer | FAM channel |
| L858R Cov Tshuaj Tiv Thaiv | FAM channel |
| 19del Reaction Buffer | FAM channel |
| T790M Cov Tshuaj Tiv Thaiv | FAM channel |
| G719X Cov Tshuaj Tiv Thaiv | FAM channel |
| 3Ins20 Cov Tshuaj Tiv Thaiv | FAM channel |
| L861Q Cov Tshuaj Tiv Thaiv | FAM channel |
| S768I Cov Tshuaj Tiv Thaiv | FAM channel |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | Kua: ≤ -18 ℃ Hauv qhov tsaus ntuj |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | cov nqaij mob tshiab, seem mob khov, cov nqaij mob lossis seem uas muab tso rau hauv paraffin, plasma lossis serum |
| CV | <5.0% |
| LoD | Kev kuaj pom cov tshuaj tiv thaiv nucleic acid nyob rau hauv keeb kwm yav dhau ntawm 3ng / μL qus-hom, tuaj yeem ntes tau 1% kev hloov pauv sai; b) nucleic acid reaction Nyob rau hauv 1% mutation rate, 1 × 103 Copies / mL mutation tuaj yeem pom tau ruaj khov hauv keeb kwm yav dhau los ntawm 1 × 105 Copies / mL; c) Cov tshuaj tiv thaiv sab hauv tuaj yeem ntes tau cov khoom siv SW3 nrog qhov txwv qis tshaj plaws ntawm qhov siv sab hauv ntawm lub tuam txhab. Thaum lub cuab yeej ntes tau cov khoom siv txwv tsis pub ntes hauv tebchaws, cov khoom siv txwv tsis pub ntes 2.5% kev hloov pauv zaus thiab kev hloov pauv ntawm EGFR gene amplification nrog tus lej theej ntawm 4 tuaj yeem ntes tau ruaj khov. |
| Kev Tshwj Xeeb | Tsis muaj kev sib tshuam nrog cov DNA genomic tib neeg qus thiab lwm hom mutant |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm, Applied Biosystems 7300 Cov Txheej Txheem PCR Tiag-Lub Sijhawm, QuantStudio® 5 Cov Txheej Txheem PCR Tiag-Lub Sijhawm, Lub tshuab LightCycler® 480 Real-Time PCR, BioRad CFX96 Real-Time PCR System. |
Kev Ua Haujlwm
Cov tshuaj reagents xav tau tab sis tsis tau muab:Dej Tsis Muaj DNase/RNase, anhydrous ethanol. Thaum kuaj cov qauv ntaub so ntswg uas muab tso rau hauv paraffin, nws raug nquahu kom siv QIAamp DNA FFPE Tissue Kit (56404) los ntawm QIAGEN thiab Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) los ntawm Tiangen Biotech (Beijing) Co., Ltd.. Thaum kuaj cov qauv plasma/serum, nws raug nquahu kom siv Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (uas siv tau nrog Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) los ntawm Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. lossis QIAamp Circulating Nucleic Acid Kit (55114) los ntawm QIAGEN.
Cov khoom siv xav tau tab sis tsis tau muabCov Lus Qhia Tsis Muaj DNase/RNase, cov hnab looj tes pov tseg, lub raj Centrifuge Tsis Muaj DNase/RNase, 8-lub raj strips rau PCR, centrifuge.
