Tib Neeg Cy2C9 thiab Vkorc1 Gene Polymorphism
Khoom npe
HWTS-ge014a-tib neeg CYP2C9 thiab VKORC1 Gene Polymorphism nrhiav khoom siv (fluorescence pcr)
Daim ntawv muaj cai
CE / TFDA
Kev kawm
Warfarin yog qhov ncauj tshuaj tiv thaiv kab mob ib txwm siv rau kev tshawb fawb tam sim no, uas yog feem ntau yog rau kev tiv thaiv thiab kho cov kab mob tshwm sim. Txawm li cas los xij, Warfarin muaj lub qhov rai kho mob tsawg thiab nws sib txawv ntau ntawm cov sib txawv sib txawv thiab cov tib neeg. Txheeb cais tau qhia tias qhov sib txawv ntawm cov koob tshuaj nyob ruaj khov hauv cov tib neeg sib txawv tuaj yeem ntau dua 20 zaug. Cov tshuaj tiv thaiv tsis zoo tshwm sim hauv 15.2% ntawm cov neeg mob noj cov neeg mob warfarin txhua xyoo, ntawm qhov uas tau muaj kev tiv thaiv kom tuag taus. Pharmacoggenomic kev tshawb fawb tau pom tias noob neej ua noob ces yog qhov tseem ceeb tshaj plaws cuam tshuam rau qhov sib txawv hauv koob tshuaj Warfarin. Warfarin yog ib qho tshwj xeeb uas muaj cov tshuaj vitamin K Epoxide Reductase (vkorc1), thiab yog li inhibits txhaws cov khoom siv cuam tshuam nrog cov vitamin K thiab muab kev tiv thaiv kev tiv thaiv. Coob tus ntawm cov kev tshawb fawb tau qhia tias cov gene polymorphism ntawm Vkorc1 Promoter yog qhov tseem ceeb tshaj plaws cuam tshuam rau kev sib tw thiab tus kheej sib txawv ntawm qhov xav tau koob tshuaj tiv thaiv kev ua rog. Warfarin yog metabolized los ntawm CyP2C9, thiab nws cov merrants heev qeeb metabolism ntawm Warfarin. Cov tib neeg uas siv Warfarin muaj kev pheej hmoo ntau dua (ob zaug rau peb zaug siab dua) ntawm los ntshav hauv kev siv thaum ntxov.
Tshooj
| Tshaib plab | VKORC1 (-1639G> A) |
| Cy5 | CYP2C9 * 3 |
| Vic / hex | IC |
Kev tsis muaj
| Chaw ntim khoom | Cov kua: ≤-18 ℃ |
| Txee-Lub Neej | 12 lub hlis |
| Zauv Hom | Fresh Edta Anticoagulated Ntshav |
| CV | APR5.0% |
| Lub lab | 1.0ng / μl |
| Yam tshwj xeeb | Tsis muaj tus ntoo khaub lig-reactivity nrog lwm txoj kev sib txawv ntawm tib neeg gy2c19 gyee, tib neeg RPN2; Kev sib hloov ntawm CyP2C9 * 13 thiab vkorc1 (3730G> a) sab nraud kev kuaj pom ntau yam ntawm cov khoom siv no |
| Muaj cov cuab yeej siv tau | Siv biosystems 7500 REO-lub sijhawm PRR cov tshuab Siv biosystems 7500 ceev real-sijhawm pc tshuab QuatStudio®5 Real-sijhawm PRR cov tshuab Slan-96p ROOST-SICTICE PRRY Lightcycler ®480 REO-SIJHAWM PCR system LINEGENE 9600 PLUS ROOST-LUB SIJ HAWM-LUB CEV Ma-6000 tiag tiag-lub sij hawm pestitive thermal cycler Biorad Cfx96 REO-SIJHAWM PCR system Biorad Cfx Open 96 Real-Time Pricr System |
Ua Haujlwm Ntws
Pom zoo rho tawm reagent: Macro & RNO KIT (HWTS-3004-32, HWTS-3004-32, HWTS-3004-32, HWTS-3004-32 3006).
