Tib neeg CYP2C9 thiab VKORC1 Gene Polymorphism
Lub npe khoom
HWTS-GE014A-Tib Neeg CYP2C9 thiab VKORC1 Gene Polymorphism Detection Kit (Fluorescence PCR)
Daim Ntawv Pov Thawj
CE/TFDA
Kev Tshawb Fawb Txog Kab Mob Sib Kis
Warfarin yog ib yam tshuaj anticoagulant uas siv rau hauv kev kho mob tam sim no, uas feem ntau yog siv rau kev tiv thaiv thiab kho cov kab mob thromboembolic. Txawm li cas los xij, warfarin muaj lub sijhawm kho mob tsawg thiab sib txawv ntawm ntau haiv neeg thiab cov tib neeg. Cov ntaub ntawv tau qhia tias qhov sib txawv ntawm cov koob tshuaj ruaj khov hauv cov tib neeg sib txawv tuaj yeem ntau dua 20 zaug. Cov kev tsis zoo tshwm sim los ntshav tshwm sim hauv 15.2% ntawm cov neeg mob noj warfarin txhua xyoo, uas 3.5% tsim kev los ntshav tuag taus. Kev tshawb fawb pharmacogenomic tau qhia tias cov noob caj noob ces polymorphism ntawm lub hom phiaj enzyme VKORC1 thiab metabolic enzyme CYP2C9 ntawm warfarin yog ib qho tseem ceeb uas cuam tshuam rau qhov sib txawv ntawm cov koob tshuaj ntawm warfarin. Warfarin yog ib qho tshwj xeeb inhibitor ntawm vitamin K epoxide reductase (VKORC1), thiab yog li inhibits clotting factor synthesis uas muaj vitamin K thiab muab anticoagulation. Ntau ntau ntawm kev tshawb fawb tau qhia tias cov noob caj noob ces polymorphism ntawm VKORC1 promoter yog qhov tseem ceeb tshaj plaws uas cuam tshuam rau haiv neeg thiab cov tib neeg sib txawv ntawm cov koob tshuaj uas xav tau ntawm warfarin. Warfarin raug metabolized los ntawm CYP2C9, thiab nws cov mutants ua rau qeeb qeeb ntawm kev metabolism ntawm warfarin. Cov tib neeg siv warfarin muaj kev pheej hmoo siab dua (ob npaug mus rau peb zaug ntau dua) ntawm kev los ntshav thaum ntxov ntawm kev siv.
Channel
| FAM | VKORC1 (-1639G>A) |
| CY5 | CYP2C9*3 |
| VIC/HEX | IC |
Cov Kev Ntsuas Kev Txawj
| Kev Khaws Cia | Kua: ≤-18 ℃ |
| Lub sijhawm khaws cia | 12 lub hlis |
| Hom Qauv | Cov ntshav EDTA tshiab tiv thaiv kev coagulation |
| CV | ≤5.0% |
| LoD | 1.0ng/μL |
| Kev Tshwj Xeeb | Tsis muaj kev sib tshuam hla nrog lwm cov kab ke sib xws ntawm tib neeg genome (tib neeg CYP2C19 gene, tib neeg RPN2 gene); kev hloov pauv ntawm CYP2C9 * 13 thiab VKORC1 (3730G> A) sab nraum qhov ntau ntawm cov khoom siv no |
| Cov Cuab Yeej Siv Tau | Applied Biosystems 7500 Cov Txheej Txheem PCR Tiag-Lub Sijhawm Applied Biosystems 7500 Cov Txheej Txheem PCR Ceev Ceev Cov Txheej Txheem PCR QuantStudio®5 Real-Time SLAN-96P Cov Txheej Txheem PCR Real-Time Lub tshuab LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Lub Tshuab Thermal Cycler Uas Siv Tau Tiag Tiag BioRad CFX96 Lub Tshuab PCR Tiag-Lub Sijhawm BioRad CFX Opus 96 Lub Tshuab PCR Tiag-Lub Sijhawm |
Kev Ua Haujlwm
Cov tshuaj reagent uas pom zoo kom rho tawm: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
