Vancomycin-resistant Enterococcus thiab Tshuaj-resistant Gene
Khoom npe
HWTS-OT090-Vancomycin-resistant Enterococcus thiab Tshuaj-resistant Gene Detection Kit (Fluorescence PCR)
Kab mob sib kis
Resistance to Drug kuj tseem hu ua tshuaj tiv thaiv, hais txog kev tiv thaiv ntawm cov kab mob mus rau kev ua ntawm cov tshuaj tua kab mob.Thaum cov tshuaj tiv thaiv tshwm sim, cov tshuaj tua kab mob ntawm cov tshuaj yuav raug txo kom tsawg.Resistance to Drug muab faib ua intrinsic resistance thiab acquired resistance.Intrinsic resistance yog txiav txim los ntawm cov kab mob chromosomal noob, dhau ntawm tiam mus rau tiam, thiab yuav tsis hloov.Tau txais kev tiv thaiv yog vim qhov tseeb tias tom qab kev sib cuag nrog cov tshuaj tua kab mob, cov kab mob hloov lawv tus kheej txoj kev metabolic kom tsis txhob tua cov tshuaj tua kab mob.
Vancomycin tsis kam genes VanA thiab VanB tau txais cov tshuaj tiv thaiv, ntawm cov VanA qhia cov qib siab ntawm kev tiv thaiv vancomycin thiab teicoplanin, VanB qhia ntau theem ntawm kev tiv thaiv vancomycin, thiab rhiab rau teicoplanin.Vancomycin feem ntau siv los kho cov kab mob Gram-zoo kab mob, tab sis vim qhov tshwm sim ntawm vancomycin-resistant enterococci (VRE), tshwj xeeb tshaj yog enterococcus faecalis thiab enterococcus faecium, suav txog ntau dua 90%, nws tau coj cov teeb meem tshiab rau kev kho mob. .Tam sim no, tsis muaj tshuaj tua kab mob tshwj xeeb rau kev kho VRE.Dab tsi ntxiv, VRE tuaj yeem xa cov tshuaj tiv thaiv kab mob mus rau lwm yam enterococci lossis lwm yam kab mob Gram-zoo.
Channel
FAM | Vancomycin-resistant enterococci (VRE): Enterococcus faecalis thiab Enterococcus faecium |
VIC/HEX | Internal Control |
CY 5 | Vancomycin Resistance gene VanB |
ROX | Vancomycin kuj gene VanA |
Technical Parameters
Cia | Ua kua: ≤-18 ℃ |
Txee lub neej | 12 hli |
Hom Qauv | hnoos qeev, ntshav, zis los yog cov kab mob ntshiab |
CV | ≤ 5.0% |
Ct | ≤36 |
LoD | 103CFU / ml |
Qhov tshwj xeeb | Tsis muaj cov kab mob sib kis nrog lwm cov kab mob ua pa xws li klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxylusa, A. legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, ua pa adenovirus, lossis cov qauv muaj lwm cov tshuaj tiv thaiv kab mob CTX, mecA, SME, Cov Qauv ntawm SHV thiab TEM. |
Cov cuab yeej siv tau | Siv Biosystems 7500 Real-Time PCR System SLAN-96P Real-Time PCR Systems LightCycler® 480 Real-Time PCR system |
Ua hauj lwm Flow
Pom zoo extraction reagents: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) thiab Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) .